This study was carried out to analyze the heterotic groups and heterotic pattern of inbred lines form maize landraces in order to provide the theoretical basis for assessing potential value of inbred lines and preparing dominant hybrid seed. The materials which were classified by using SSR markers were 11 testers (inclusive: inbred lines of using commonly in southwest regions, 5 normal corn inbred lines which represented five main heterotic groups), and 17newly inbred lines form maize landraces. The materials of estimating heterotic patterns were 187 combinations by crossing 17 newly maize inbred lines with 11 testers by NCⅡ mating design. Clustering analysis was done on the basis of the genetic similarity. Heterotic patterns were analyzed based on the data from the experiment and clustering analysis. The results showed that the 28 inbred lines could classified into 4 heterotic groups, group named Sipingtou contained 3 newly inbred lines, group named PB contained 4 newly inbred lines, group named Reid contained 3 newly inbred lines, and group named Luda Red Cob contained 7 newly inbred lines. Among the various populations, PB(P1-1, P1-8, and P1-10)× Reid and PB(P1-1, P1-6, and P1-14)× Luda Red Cob and Reid(P1-15, P1-16, and P1-17)×Sipingtou could become superior combinations, especially newly inbred line P1-1 × Reid become heterotic pattern. The heterotic groups analysis of inbred lines provided the basis for application of 17 newly inbred lines and improvement of maize landraces.