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Expression and Function of Cauliflower BobERF17, A Member of the AP2/ERF Transcription Factor Family, In Response To Abiotic Stresses
LI Hui, YANG Yaling, LI Cong, LI Lihong, HAN Zhanpin, WANG Chunguo
2021, 35 (8): 1794-1801. DOI: 10.11869/j.issn.100-8551.2021.08.1794
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AP2/ERF is a plant-specific transcription factor superfamily, among which the members of ERF family have been identified to play important roles in abiotic stress responses in plants. To further understand the role of ERFs in cauliflower, in the present study, BobERF17, a member of the ERF family in cauliflower, was cloned, and the transcriptional expression and function of BobERF17 were further explored. Sequence analysis showed that BobERF17 coding region is 576 bp in length and encodes a protein consisting 191 amino acids with unknown function. Cluster analysis showed that BobERF17 had a high similarity to the ERF17 sequences in dicotyledons, especially Brassica plants, but differed greatly from the ERF17 transcription factors in monocotyledons. Analysis of expression profiles under different stress conditions confirmed that BobERF17 displayed significantly up-regulated expression characteristics under drought and high temperature stresses, but was not sensitive to salt and low temperature stresses. Subsequently, the BobERF17 overexpression vector was successfully constructed and transformed into Arabidopsis. Overexpressed BobERF17 Arabidopsis homozygous lines were obtained. Functional analysis showed that the overexpression of BobERF17 in Arabidopsis did not affect the growth and development of the transgenic lines, but could significantly increase their tolerance to drought and high temperature stresses. The results confirmed that BobERF17 may play an important role in positive response to drought and high temperature stresses in cauliflower. These findings provided new insight into the function and regulation of BobERF17 in cauliflower.
Research Progress on Promoting Growth and Drought Resistance of Wheat by Plant Growth Promoting Rhizobacteria
WEN Hongwei, YANG Bin, WANG Dongsheng
2021, 35 (9): 2194-2203. DOI: 10.11869/j.issn.100-8551.2021.09.2194
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Drought stress is the most important abiotic stress factor affecting the growth and development and yield formation of wheat. With the adverse effects of excessive use of chemical fertilizer and global climate change, the harm of drought stress in the growth of wheat is becoming more and more serious. Plant growth promoting rhizobacteria (PGPR) can not only help wheat improve its nutrient utilization efficiency and promote its growth, but also help wheat to resist drought stress through its own action or various metabolites. In this paper, the mechanism of PGPR promoting growth was summarized, and the research progress of PGPR in improving wheat drought resistance was summarized from two aspects of physiological and molecular mechanism, which laid a theoretical foundation for the application research of using PGPR to improve wheat growth promotion and drought resistance.

Effects of Biochar-Attapulgite Composite on Zinc and Cadmium Passivation of Paddy Soil and Soil Nutrient and Enzyme Activities
YOU Lingcong, WANG Yuying, LIU Yuxue, LYU Haohao, CHEN Jinyuan, YANG Shengmao
2021, 35 (7): 1717-1723. DOI: 10.11869/j.issn.100-8551.2021.07.1717
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In order to compare the remediation effect of biochar, attapulgite and attapulgite-biochar composite. FTIR, XRD, BET and SEM were used to characterize rice husk biochar and attapulgite samples, and soil incubation experiments were conducted to study the effects of rice husk biochar, attapulgite and attapulgite-biochar composites on the passivation effect and physicochemical properties of Zinc and Cadmium contaminated soil. The incubation results showed that these three passivation agents reduced the bioavailability of Cd and Zn, meanwhile improved the soil properties to varying degrees. Zn content was reduced by 86% and 51%, and Cd content was reduced by 25% and 8% for the treatment of 2.0% attapulgite and 2.0% biochar, respectively. The availabe content of Zn and Cd in the soil was reduced by 83% and 23%, respectively, the soil pH was increased to 5.8, and the organic carbon content increased by 39%. after the application of 2.0% biochar-attapulgite composite. Compared with individual applications of attapulgite and biochar, rice husk biochar-attapulgite composite could efficiently immobilize Cd and Zn, and improve physicochemical properties in Zinc and Cadmium contaminated paddy-growing soil. Therefore, attapulgite-biochar composite could be a potential new passivation agent for soil remediation providing some new ideas for soil heavy metal remediation.
Genetic Diversity Analysis of Sesame Germplasm Resourcesin Shanxi With SSR Markers
LYU Wei, HAN Junmei, REN Guoxiang, WEN Fei, WANG Ruopeng, LIU Wenping
2021, 35 (7): 1495-1506. DOI: 10.11869/j.issn.100-8551.2021.07.1495
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In order to explore the genetic characteristics of sesame germplasm resources in Shanxi, 30 pairs of simple sequence repeat (SSR) were used to analyze the genetic diversity and population structure of 71 sesame germplasms of Shanxi in this study. The results showed that a total of 144 allelic loci were detected with 30 pairs of SSR markers, the mean number of alleles per locus was 4.800; the number of effective alleles ranged from 1.058 to 5.149, with an average of 2.805; the index of Shannon ranged from 0.128 to 1.813, with an average of 1.096; the genetic diversity index of Nei's varied from 0.055 to 0.806, with an average of 0.558; the polymorphic information content ranged from 0.053 to 0.783, with an average of 0.515. Based on cluster analysis with SSR molecular markers, the genetic similarity coefficient of the tested materials was 0.21~0.67, and six groups were clustered at the genetic similarity coefficient of 0.27; The population structure analysis based on SSR molecular markers divided the test materials into five groups. The results showed that the genetic difference among sesame germplasm resources in Shanxi was relatively high and there were rich genetic diversities. Development and utilization of sesame germplasm resources of Shanxi might lay a good foundation for the genetic improvement and discovery of excellent genes of sesame in future.
Comprehensive Evaluation of Natural Compound Salt Stress of Sea-Island Cotton Resources
YANG Tao, LI Shengmei, HUANG Yajie, REN Dan, CUI Jinxin, PANG Bo, GAO Wenwei
2021, 35 (7): 1507-1521. DOI: 10.11869/j.issn.100-8551.2021.07.1507
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In order to screen out the sea-island cotton varieties suitable for saline-alkali land cultivation, 203 sea-island cotton were selected and evaluated under natural compound salt stress. The results showed that plant height, height of the first node, fruit branch numbers, number of boll drop, proportion of boll drop and salt tolerance coefficient of yield of Single boll seed cotton were decreased. Lint percent, boll numbers, first node of fruit branch, effective boll numbers, effective fruit branch numbers, yield of single boll lint yield, seed cotton yield per plant and lint yield per plant were increased slightly. The coefficient of variation showed that the seed cotton yield per plant and lint yield per plant were the highest. The correlation analysis showed that there was a significant positive correlation between fruit branch numbers and boll numbers; proportion of boll drop has significant correlations with boll number, height of the first node, first node of fruit branch, fruit branch number, effective fruit branch numbers, effective boll numbers, number of boll drop. Yield of Single boll Lint yield has significant correlations with lint percent, boll numbers, fruit branch numbers, effective boll numbers, effective fruit branch numbers, number of boll drop, proportion of boll drop, yield of Single boll seed cotton, yield of Single boll Lint yield, seed cotton yield per plant. The correlation analyses of the lint yield per plant identification value, the principal component score, the membership value and the comprehensive score D value showed that the four were significant positive correlation ( P<0.01), indicating that sea-island cotton could be comprehensively evaluated and classified by the four comprehensive scores. The sea-island cotton resources are mainly salt-tolerant and medium salt-tolerant, with relatively few extreme materials. Through comparison, it is found that the loose fruit branch type is more salt-tolerant than the compact type. In addition, a variety Pima 4 with strong compound salt tolerance was selected. The salt tolerance indexes of 6 sea-island cotton were screened by stepwise regression. The results of this experiment provide a certain theoretical basis, identification indicators and methods for the screening of sea-island cotton varieties tolerant to compound salt in the boll stage.
Biochar Concept Connotation and Phrases Discrimination
SHENG Kuichuan, YANG Shengmao
2022, 36 (2): 481-487. DOI: 10.11869/j.issn.100-8551.2022.02.0481
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The Chinese expression of the terminology “biochar” has created arguments in academic world for many years. This paper looks insight into the concept of biochar and analyzes the confusion nomenclature phenomena, based on the basic method of logistics on concept, phrases, definition and classification. By clarifying the logic problems on the definition and classification of biochar, this work established good practice for the in-depth understanding of biochar terminology, and promoting the spread of the related scientific knowledge and the application of the technological achievements.

Traits Analysis of Offsprings Mutated by 60Co-γ Radiation in Iris hollandica
LIN Bing, FAN Ronghui, CHEN Yuhua, FANG Nengyan, YE Xiuxian, ZHONG Huaiqin, HUANG Minling
2021, 35 (10): 2205-2213. DOI: 10.11869/j.issn.100-8551.2021.10.2205
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In order to explore distinguishing main characters of mutant obtained by 60Co-γ radiation in Iris hollandica Zhanchi, botanical agronomic traits of 4 stable mutants (5-7CBS, 7-10SLZ, 7-10QLS, 10ZLL) and their wild-type Zhanchi were evaluated, the similarities and differences of the main ornamental traits (flowers) were analyzed and compared according to test guidelines of Bulbous Irises. It was found that mutants and wild type were basically consistent in plant morphology, flowering period and growth period. Among the 40 traits investigated, there were significant differences in the characteristics of five pseudo-qualitative characteristics between mutants and wild type, such as groud color of upper side of blade in outer tepal, color of inner tepal, color of upper side in bridge, color of upper side in crest, and groud color of upper side of claw in outer tepal. Another 35 character were highly similar in corresponding phenotype and values. The results showed that flower color of Iris hollandica has great genetic improvement potetiality with radiation breeding. It is speculated that flower color variation may be an important indicator for distinguishing mutants from wild type. According to the expression state of flower color, variation of flower color can be divided into four types: strengthening type, wild type, weakening type and full variant type. This study can provide basic data and reference for breeding new varieties in Iris hollandica.

Rapid Detection of Three Foodborne Pathogens Based on SERS
HUANG Dongwei, WANG Tiantian, GU Guizhang, ZHANG Jinjie, XU Dalun, YANG Wenge
2021, 35 (7): 1619-1626. DOI: 10.11869/j.issn.100-8551.2021.07.1619
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In order to explore the feasibility of Surface-enhanced Raman Spectroscopy (SERS) method for rapid detection of Escherichia coli (ATCC 25922), Salmonella enteritidis (CICC 21540) and Listeria monocytogenes (CICC 10982), a two-step growth method was used to prepare silver-coated gold nanoparticles (Au@Ag NPs) as an enhanced substrate in this study. The Raman enhancement effect and stability of Au@Ag NPs coupled with three pathogenic bacteria were studied respectively. The characteristic Raman spectroscopy of the pathogenic bacteria was comparatively analyzed with the combination with principal component analysis (PCA) and hierarchical clustering analysis (HCA). The results showed that the synthesized Au@Ag NPs had uniform particle size, obvious Raman enhancement effect, which could effectively combine with the 3 kinds of pathogenic bacteria to generate stable Raman response; PCA and HCA analysis based on the characteristic Raman spectra of Escherichia coli, Salmonella enteritidis and Listeria monocytogenes showed that the Raman characteristic spectra of the three pathogenic bacteria were obviously different and could be effectively distinguished. The results of this study provide a reference basis for the rapid detection of E. coli, S. enteritidis and L. monocytogenes by using SERS method with Au@Ag NPs as the enhanced substrate.
Screening and Evaluation of Cucumber Salt-Tolerant Germplasm
CAO Qiwei, DU Lianda, YANG Zonghui, LI Libin, DUAN Xi, YANG Wenqiang, CHEN Wei, MENG Zhaojuan
2022, 36 (5): 865-875. DOI: 10.11869/j.issn.100-8551.2022.05.0865
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In order to clarify the different tolerance of cucumber germplasm to NaCl, the salt-tolerant material MC2065 and salt-sensitive material White cucumber were used to verify the appropriate concentration of 50, 100, 150, 200, and 250 mM NaCl. And nine physiological and biochemical indexes, including salt tolerance index, chlorophyll, and protective enzymes under NaCl treatment were used to evaluate the salt-tolerances of 21 germplasms. The results showed that 100 and 150 mM could be the appropriate concentration.The salt injury index of White cucumber was 35.94% in 100 mM NaCl, showing obvious damage to leaves, while that of MC2065 was 4.69% and showed no obvious damage. Under 150 mM, both showed obvious damage, more than half of leaves were withered for White cucumber, but less than half for MC2065. With cluster analysis, 21 germplasms were divided into 3 groups. The first group categorized as salt-tolerant germplasm, including ZQ3, N26-5-1, Shoushui 1, DRTJY-2, Laixi, Cuiyu, 20S077-1. The second group, including DJ04, DY-1, XY1, YY9123, M2XT, D1503, X805, was medium salt-tolerant germplasm. And the third group was salt-sensitive germplasm, which included F6-3-1, SJ11-1, XB23, 20S091-1, HLT-921h, AZ-1, DRT345. The trend of comprehensive scores of cucumber germplasm by the compoment analysis was basically consistent with that obtained by cluster analysis and seedlings survival rate. The study will provide references for investigating cucumber salt-tolerant mechanism and lay a foundation for the breeding of cucumber salt-tolerant varieties.

Effects of Several Environmental Factors on Nutrient Accumulations of Nannochloropsis oceanica
ZHANG Yuanbo, TIAN Jiaojiao, YE Lingzhi, YE Zhengwei, ZHANG Lin, XU Jilin
2022, 36 (6): 1273-1283. DOI: 10.11869/j.issn.100-8551.2022.06.1273
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To explore effects of light intensity, light quality, nitrogen, and phosphorus on nutrient accumulations inNannochloropsis oceanica, this study was designed into four parts as followed. The light intensity was set as 20, 80, 140 and 200 μmol photons·m-2·s-1, respectively, in the light intensity experiment. In the part of light quality, ratios of red-blue-green light were adjusted to 100%, 50% and 0%, respectively, according to the RGB principle. Nitrogen concentration was set as 55.40, 13.85, 3.46 and 1.73 mg·L-1 in the nitrogen experiment, respectively. Phosphorus concentration was set to 8.96, 2.24, 0.56 and 0.28 mg·L-1 in the phosphorus experiment, respectively. The initial inoculation density was unified and the composition of fatty acids, pigment, protein, and soluble sugar were measured after 25 days. At the light intensity of 200 μmol photons·m-2·s-1, N. oceanicaobtained the fastest growth rate, the highest biomass; besides, the concentration of protein (5.17 mg·mL-1), and soluble sugar (2.40 mg·mL-1) is the greatest. At the light intensity of 20 μmol photons·m-2·s-1, the ratio of chlorophyll a was the highest. Overall, the contents of protein and soluble sugar increased with light intensity. The light quality experiment showed that the red light had a certain effect on promoting the growth and soluble sugar accumulation, while blue and green lights did not. Results also suggested that the green light was conducive to β-carotene accumulation, but 100% green light significantly inhibited the growth of N. oceanica. At the nitrogen concentration of 55.40 mg·L-1, the proportions of unsaturated fatty acids and eicosapentaenoic acid (EPA) of N. oceanicareached the maximum, which were 68.23% and 20.50%, respectively. With the nitrogen concentration declining, the growth rate, protein, and soluble sugar inN. oceanicasignificantly decreased. Similarly, with the phosphorus concentration declining, the growth rate and protein content significantly decreased. The phosphorus concentration of 2.24 mg·L-1 was beneficial to soluble sugar accumulation. This study provides theoretical support for optimizing the cultivation of N. oceanica under different environmental factors, which has certain practical significance and economic value.

Antibacterial Activity, Mechanism and Application of Plant-Derived Natural Products in Food Preservation
XING Min, FEI Peng, GUO Ling, KANG Huaibin
2021, 35 (8): 1875-1882. DOI: 10.11869/j.issn.100-8551.2021.08.1875
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Food safety problems caused by food-borne pathogens seriously threaten the public health, which have caused widespread concern. Therefore, how to safety and effectively inhibit the growth of pathogenic bacteria in food has become a research hotspot in the food field. Current researched show that plant-derived natural products have wide sources, which possess broad antibacterial spectrum and less side effects than chemical preservatives, and can be used as natural preservatives in food. This article reviewed the antibacterial activity, mechanism and application in food preservation of plant-derived natural products, in order to provide theoretical basis for reducing food-borne pathogen contamination in food.
Metabonomics Analysis of Metabolic Difference of Hunan Tea [ Camellia Sinensis (L) O. Kuntze] Germplasm Resources
LIU Zhen, CHENG Yang, ZHAO Yang, YANG Peidi, NING Jing, YANG Yang
2022, 36 (1): 83-93. DOI: 10.11869/j.issn.100-8551.2022.01.0083
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Hunan province is located in a transitional zone of tea evolution, where tea germplasms resources are abundant. These germplasm have not only significantly different botanical characteristics, but also varied tea suitability, adaptability, cold tolerance, and etc. However, there were insufficient analyses on the component differences of tea resources in Hunan province. In order to further explore the differences in functional component of Hunan local tea germplasm, and provide the basis for mining and utilizing these tea resources. The metabolites of Chenbu Dongcha (CBDC), Jianghua Kucha (JHKC), Rucheng Baimaocha (RCBM), and Anhua population (AHQT) in Hunan province were detected using UPLC-MS/MS (with the cultivated landrace AHQT as the control group) and identified using mass spectrometry database. A total of 609 metabolites were detected in the 4 populations. Principal component analysis (PCA) showed that the metabolites among the 4 populations were significantly different. The metabolites of AHQT population had the highest similarity to that of CBDC population, and the lowest to that of RCBM populations. With AHQT population as the reference, a total of 302 differential metabolites were detected based on the OPLS-DA model, mainly including amino acids, phenolic acids, flavonoids, tannins, and alkaloids. Among which fifty common differential metabolites were detected in the 3 populations; they were mainly flavonoids and phenolic acids. There were 52, 37, and 66 unique differential metabolites that were detected in CBDC, JHKC, and RCBM, respectively. Except for some reported differential metabolism, we also detected some less concerned differential metabolites in tea research. These differential metabolisms will provide an important reference for tea germplasm excavation and breeding.

Mutagenic Effects of 60Co-γ Irradiation on Four Species of Lilium
LI Lihui, HU Yao, LEI Xingyu, ZHANG Yuelong, LI Honggao, HU Die, ZHANG Yong, DENG Gangqiao
2021, 35 (8): 1725-1730. DOI: 10.11869/j.issn.100-8551.2021.08.1725
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To investigate the mutagenic effects of different radiation doses of 60Co-γ rays on Lilium, the seedballs of Lilium lancifolium Thunb, Lilium bosniacum, Lilium henryi Baker and Lilium regale Wilson were used as test materials and treated with different dose(0,2,4,6Gy) of 60Co-γ rays. Statistical analyses of the germination rate, characters, physiological and biochemical indexes and variations.The results showed that the germination rate, plant height, bloomed rate and production of Lilium bosniacum, Lilium henryi Baker and Lilium regale Wilson gradually decreased with the increasing of radiation dosage. However, Lilium lancifolium radiated by 2 Gy showed no significant difference from the control,and by 4 Gy,the germination rate showed significant difference when compared with control. Under the radiation dose of 6 Gy,the seedballs could not sprout. 2 Gy dose radiation had little effect on the contents of chlorophyll for four varieties, 4 Gy treatment showed a downward trend.The activity of superoxide dismutase (SOD) increased first and then decreased in all varieties. While the malondialdehyde (MDA) increased gradually. The soluble sugar content of Lilium lancifolium Thunb increased first and then decreased, and other varieties increased with the increase of dose. The suitable radiation dose ranges for Lilium bosniacum, Lilium henryi Baker and Lilium regale Wilson were 2 to 4 Gy, 2 Gy and 2 Gy respectively. No appropriate dose was found for the Lilium lancifolium Thunb in this experiment. The results provide some technical references to a certain extent for Lilium radiation breeding.
Drought Resistance Evaluation Based on Leaf Anatomical Structure of Major Chestnut Cultivars in Beijing-Tianjin-Hebei Region
GUO Yan, ZHANG Shuhang, LI Ying, ZHANG Xinfang, WANG Guangpeng
2021, 35 (8): 1771-1782. DOI: 10.11869/j.issn.100-8551.2021.08.1771
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To explore the evaluation method of drought resistance of Chestnut cultivars. In this study, 21 anatomical structure indexes of 36 chestnut cultivars were measured by using multi-function image analysis, frozen sectioning and nail oil seal method combined with the determination of leaf water retention capacity. Leaf anatomical structure indexes were screened by variance analysis and principal component analysis, and comprehensive evaluation on drought resistance of each cultivar was conducted by the subordinate function.The results showed that 18 anatomical structure indexes of the 21 indexes all reached extremely significant differences among the 36 cultivars ( P<0.01), and 6 typical indexes including leaf thickness, thickness of palisade tissue, the total stoma perimeter per unit area, petiole length, thickness of upper epidermis cell and spacing length of veins were selected from the 18 indexes by principal component analysis. The drought resistance level of 36 cltivars were divided into 5 types according to the measurement value of drought resistance ( D)calculated by the membership function method combined with 6 typical indexes and their weights, 4 cultivars were divided into high drought resistance types(HR),the D value ranged from 0.60 to 0.80; 10 cultivars were divided into drought resistance types(R), the D value ranged from 0.50 to 0.59; 13 cltivars were divided into medium drought resistance types(MR), the D value ranged from 0.30 to 0.49; 6 cltivars were divided into low drought resistance types(LR),the D value ranged from 0.20 to 0.29; 3 cltivars were divided into susceptible types(S),the D value ranged from 0 to 0.19. The classification of 36 chestnut cultivars based on the drought resistance measurement were consistent with the evaluation of leaf water retention. This study provided basis for the drought resistance type division and scientific utilization of chestnut cultivars in Beijing-Tianjin-Hebei region.
Accumulation and Partitioning Characteristics of Photosynthetic Product of 7 Main Cultivars in Northern Winter Wheat Zone
YANG Shurong, FU Luping, FEI Shuaipeng, LI Simin, CHEN Xinmin, XIA Xianchun, XIAO Yonggui, MENG Yaxiong
2021, 35 (8): 1740-1750. DOI: 10.11869/j.issn.100-8551.2021.08.1740
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The objectives of the present study were to analyze the dry matter accumulation and partitioning characteristics of wheat cultivars in the Northern Winter Wheat Zone (NWWZ). Seven elite cultivars from the NWWZ during the past 20 years were selected and planted at two experimental sites in two cropping seasons. The dynamic accumulation and transportation of dry matter in different organs during grain-filling, harvest index (HI), and grain yield of all cultivars were investigated. The results showed that the HI and biomass had significant effects on yield. Zhongmai 816 and Zhongmai 175 had higher yield potential, which were 4 923.0 kg·hm -2 and 4 913.0 kg·hm -2, respectively, mainly attributed to their higher biomass yield and HI. The analyses of dry matter accumulation revealed that photosynthetic area at seedling stage and the photosynthetic utilization efficiency during grain filling stage had important effects on dry matter accumulation. Cultivars with low chlorophyll content, small flag leaf area (FLA), and high photosynthetic rate (Pn) during the grain filling stage had higher dry matter accumulation efficiency and more soluble sugar storage in stems and leaves, such as Zhongmai 175. In term of the dry matter distribution in vegetative organs, the dry matter storage of vegetative organs in Zhongmai 816 and Zhongmai 175 at the flowering stage was slightly different from that of other cultivars, but the dry matter transportation efficiency in stems and leaves at the maturity stage was higher, and the residues of stored matter was less. In addition, the photosynthetic compounds stored in Zhongmai 175 and Zhongmai 1062 before flowering had the highest contributions to grains than other cultivars. That demonstrated that improving the light energy utilization efficiency of cultivars was a feasible way to increase the genetic yield potential. In addition, the selection of dry matter accumulation before the flowering stage and the dry matter transportation efficiency at grain filling stage is helpful to reduce the residual photosynthetic products in tissues and organs at maturity, and improve the efficiency of transporting substance to grains. This study can determine the future breeding improvment target and direction for new wheat cultivars in the NWWZ.
Principal Component and Cluster Analysis of Volatile Components in Cape Jasmine Flower From Different Cultivars at Different Stages of Bloom
LU Lulu, FAN Yiling, DENG Ke, XU Guangzhi, WANG Yan, ZHANG Youzuo, NI Qinxue
2021, 35 (7): 1601-1608. DOI: 10.11869/j.issn.100-8551.2021.07.1601
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Principal component analysis (PCA) and cluster analysis (CA) were used to investigate and classify the volatile components of cape jasmine flower from different cultivars including Gardenia jasminoides Ellis, Gardenia jasminoides radicans, Gardenia stenophylla Merr., and Gardenia jasminoides var. Fortuniana Lindl. at three stages of bloom, namely the early (stage Ⅰ), the full (stage Ⅱ) and the late (stage Ⅲ) flowering stages expansion. Headspace solid phase microextraction-gas chromatograohy-mass spectrometry(HS-SPME-GC-MS) was used to analyze the volatile components of 12 samples of cape jasmine flower. 52 kinds of volatile components in Cape jasminoides flower were obtained, and that 7 principal components was extracted using PCA analysis could reflect most of the information on the samples with a total cumulative variance contribution rate as high as 90.453%. 12 samples of cape jasmin flower samples could be divided into two clusters by PCA and CA. One of the clusters was formed by 5 cape jasmine flower samples collected from stage Ⅰ of Gardenia jasminoides Ellis, stage Ⅰ of Gardenia jasminoides var. Fortuniana Lindl and three stages of Gardenia jasminoides var. radicans. The other cluster was made up of the remaining 7 cape jasminoides flower samples. Gardenia jasminoides belonging to the same cluster showed silmilar flavor. This study could be an experimental reference for the development and utilization of cape jasmine flower resources.
Role of Sucrose Metabolism and Signal Transduction in Plant Development and Stress Response
FENG Yalan, YIN Fei, XU Ke, JIA Xiaoyi, ZHOU Shuang, MA Chao
2021, 35 (9): 2044-2055. DOI: 10.11869/j.issn.100-8551.2021.09.2044
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Sucrose metabolism plays a critical role in plant development, stress response and yield formation. The growth and development process of the plant is promoted through the synthesis and decomposition of a series of sugars. As a signal factor, it participates in regulating the expression of related genes, and can interact with other genes, hormones, and defense signals. However, the coupling mechanism of sucrose metabolism and signal transmission between intracellular and external is different from the signal transduction mechanism of sucrose metabolizing enzymes. This article reviews the function of crucial sucrose metabolism enzymes in plant development and response to abiotic stresses. Combining with the current researches, the future research direction of sugar metabolism and its signal transduction has being proposed, which will contribute to understanding and improving the characteristics of plant growth and development as well as stress resistance.

Cloning and Expression Analysis of Sorghum Transcription Factor SbWRKY71 Gene Under Stress
DU Qiaoli, JIANG Junmei, CHEN Meiqing, FANG Yuanpeng, LI Xiangyang, REN Mingjian, XIE Xin
2021, 35 (7): 1532-1539. DOI: 10.11869/j.issn.100-8551.2021.07.1532
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In order to explore the important role of WRKY transcription factors in plant resistance to adversity stress. In this study, a WRKY transcription factor gene ( SbWRKY71) was cloned from Sorghum BTx623. The transcription factor gene was 1 335 bp in length (Phytozome Graviton No: Sb04g005520), encoded 364 amino acids, and the molecular weight was 38.95 kDa. Through bioinformatics method to predict the transcription factor located in the nucleus, with WRKY transcription factors typical conservative structure domain, and the protein belongs to the group Ⅱ member of WRKY protein family. Phylogenetic tree analysis showed that sorghum SbWRKY71 had the closest affinity with gramineous corn ZmWRKY71, and its affinity was 75%. Quantitative real-time PCR (RT-qPCR) showed that SbWRKY71 gene expression was tissue specific, with the highest expression abundance in the leaves and the lowest in the stems. SbWRKY71 expression decreased first, then increased and then decreased after treatment with the hormone salicylic acid (SA, 1 mmol·L -1), indole-3-acetic acid (IAA 10 mol·L -1) and abscisic acid (ABA, 200 mol·L -1). Under the treatment of drought stress simulated by gamma-aminobutyric acid (GABA) and D-Mannitol (300 mmol·L -1) as well as salt stress (NaCl, 250 mmol·L -1), the gene expression of SbWRKY71 presented a pattern of first increase and then decrease. The expression of SbWRKY71 reached its maximum value at 3, 6 and 9 h respectively, after which it was significantly down-regulated. SbWRKY71 expression was inhibited after sorghum was treated with pathogen-related molecular model (PAMPs) flagellin (flg22, 100 nmol·L -1), translation elongation factor (elf18, 100 nmol·L -1) and Chitin (8 nmol·L -1). This study provides a basis for further exploring the role of SbWRKY71 gene in regulating sorghum resistance, hormone response and stress response.
Effect of Sowing Date on Agronomic Characters, Quality and Yield of New High Protein Soybean Varieties (lines)
TIAN Yixin, GAO Fengju, CAO Pengpeng, GAO Qi, XIA Wenrong
2021, 35 (8): 1900-1907. DOI: 10.11869/j.issn.100-8551.2021.08.1900
Abstract178)   HTML7)    PDF (1366KB)(233)      
The high-protein soybean varieties (lines) Shengdou 18, Shengdou 24, Hedou 37 and Hedou 38 in Huang-Huai-Hai region were used as experimental materials, and the effects of different sowing dates on the growth period, agronomic traits, dry matter accumulation, grain quality and yield of soybean varieties were studied to determine the suitable sowing date of the high-protein sybean varieties(lines) in Huang-Huai-Hai region. The results showed that the whole growth period of soybean was shortened with the delay of sowing date, and the plant height, pod height and the number of main stem nodes decreased with the delay of sowing date. The effective branching and pod number, seed number and weight of per plant, 100-seed weight per plant, dry weight of root, stem, pod and total plant, seed protein and fat content, and yield all increased first and then decreased with the delay of sowing date. With the delay of sowing date, the dry weight of pod changed greater, and the proportion of dry weight of root and stem decreased, the proportion of dry weight of pod increased. The effect of sowing date on grain protein, fat content and yield was dominant The highest yields of Shengdou 18, Shengdou 24, Hedou 37 and Hedou 38 were 3 940.95, 3 750.24, 3 252.37 and 3 905.54 kg·hm -2, respectively, when sowing on June 15, and the grain weight per plant was most closely related to the yield. From the above, the optimum sowing date can promote the reasonable transition of vegetative growth and reproductive growth, make the plant growth, dry matter accumulation and distribution reach the best state, and realize the high yield and quality of soybean, further promote the development of soybean and high-protein soybean industry.
Effects of 60Co-γ Radiation and PEG Stress on Physiological Characteristics and Related Gene Expression of Mulberry Seedlings
ZHAO Dongxiao, SHI Xinqin, DONG Yaru, GENG Bing, SUN Jingshi, LOU Qinian, WANG Zhaohong, GUO Guang
2021, 35 (7): 1485-1494. DOI: 10.11869/j.issn.100-8551.2021.07.1485
Abstract163)   HTML5)    PDF (2154KB)(229)      
To investigate the influences of different irradiation dosages of 60Co-γ and PEG stress on the physiological characteristics of mulberry seedlings,and select the optimal radiation dosage for improving the drought resistance of mulberry. Guiyou 12 seeds were irradiated with five doses of 60Co-γ rays: 0, 100, 200, 300 and 400 Gy. Under the simulative drought stress of 10% polyethylene glycol (PEG), the relative permeability of plasma membrane, the content of malonaldehyde (MDA), the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and the content of soluble protein and proline in the leaves and roots were measured and the expression levels of peroxidase gene POD1, superoxide dismutase gene sodC and pyrroline-5-carboxylic acid synthetase P5 CS which was the key enzyme of proline synthesis were detected. Results indicated that low dose 60Co-γ radiation (≤200 Gy) could reduce the relative permeability of plasma membrane and the content of MDA in the leaves and roots of mulberry seedlings, improve the SOD, POD and CAT activity, increase the content of soluble protein and proline, and up-regulate the expression levels of POD1, sodC and P5 CS, and so as to improve the drought resistance of mulberry seedlings. While the high dose of 60Co-γ radiation (≥300 Gy) aggravated the stress injury. Comprehensive analysis showed that the seeds irradiated with 200 Gy 60Co-γ had the strongest drought resistance. This study provided theretical references for mulberry cultivation and breeding in drought region.
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