Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (11): 2655-2663.DOI: 10.11869/j.issn.100-8551.2021.11.2655

• Isotope Tracer Technique·Ecology and Environment·Physiology • Previous Articles     Next Articles

Physiological Indexes and Metabolomics Changes in the Upper Stalk Tobacco Leaves Under the Delaying Harvesting in Yuzhong Area

PENG Cuiyun1(), WANG Haiyan2, SHI Hongzhi1, GUO Hui1, YANG Huijuan1,*()   

  1. 1Tobacco College, Henan Agricultural University, Zhengzhou, Henan 450002
    2Institute of Nuclear Agriculture, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310000
  • Received:2021-04-22 Accepted:2021-05-27 Online:2021-11-10 Published:2021-09-18
  • Contact: YANG Huijuan

延迟采收对豫中烤烟上部叶生理指标和代谢组学的影响

彭翠云1(), 汪海燕2, 史宏志1, 郭慧1, 杨惠娟1,*()   

  1. 1河南农业大学烟草学院,河南 郑州 450002
    2浙江大学农业与生物技术学院核农所,浙江 杭州 310000
  • 通讯作者: 杨惠娟
  • 作者简介:彭翠云,女,主要从事烟草栽培生理生化与分子生物方面研究。E-mail: pengcuiyun1203@163.com
  • 基金资助:
    河南省烟草公司许昌市公司科技项目(2018411000240044)

Abstract:

In order to explore the effects of delayed harvesting practice on the physiological indexes and metabolomics of upper leaves of Yuzhong flue-cured tobacco, Zhongyan 100 was used as experimental material to study the effects of delayed harvesting practice on antioxidant indexes, key enzyme activities of nitrogen metabolism, reducing sugar content and metabolomics of upper leaves. Results showed that with the extension of delayed harvesting time, the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in the antioxidant system of tobacco leaves were decreased gradually, while the contents of malondialdehyde (MDA) and proline (Pro) were increased compared with the normal harvesting way. Compared with conventional harvesting method, the content of reducing sugar in upper tobacco leaves reached the highest level when the harvesting was delayed for 8 days, which increased by 97.10%. The activities of nitrate reductase (NR) and glutamine synthase (GS), the key enzymes in nitrogen metabolism, decreased gradually with the prolongation of harvesting time, and reached the lowest level after 16 days’ delaying. The time series analysis (STEM) of metabolites from upper tobacco leaves under different treatments showed that the metabolites which had the similar expression patterns were clustered into one group. In addition, KEGG analysis showed that with the prolongation of harvest period, the metabolites which mainly enriched in the biosynthesis of terpenoids and steroids, the metabolism of D-glutamine and D-glutamate, the biosynthesis of unsaturated fatty acids and the biosynthesis of tropane, piperidine and pyridine alkaloids were continuously down-regulated during the whole process. The metabolites which enriched in starch and sucrose metabolic pathways, tyrosine metabolism, plant hormone biosynthesis and other metabolic pathways were down-regulated when delayed for 8 days. In conclusion, in Yuzhong area the best harvesting time for the upper tobacco leaves is 8 days’ delay. This study provides an important theoretical basis for determining the suitable harvesting time of fluc-cured tobacco upper stalk leaves and guiding the field harvest scientifically in Yuzhong area.

Key words: flue-cured tobacco, upper leaves, metabolomics, nitrogen metabolism, delayed recovery

摘要:

为探究延迟采收对豫中烤烟上部叶生理指标和代谢组学的影响,本研究以中烟100为试验材料,研究延迟采收对上部叶抗氧化性指标、氮代谢关键酶活性、还原糖含量以及代谢组学的影响。结果显示,随着延迟采收时间的延长,烟叶中抗氧化系统中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性逐渐降低,丙二醛(MDA)和脯氨酸(Pro)含量较常规采收时上升。与常规采收相比,延迟采收8 d时烟叶中还原糖含量达到最高,增加了97.10%。氮代谢中的关键酶硝酸还原酶(NR)和谷氨酰胺合成酶(GS)活性随着采收期延长逐渐降低,延迟16 d时活性达到最低。不同处理的上部叶代谢物经过时间序列分析(STEM)发现变化趋势相似的代谢物聚为一类,经过代谢途径分析(KEGG)发现,随着采收期的延长,主要富集在萜类化合物和类固醇的生物合成,D-谷氨酰胺和D-谷氨酸代谢、不饱和脂肪酸的生物合成,托品烷、哌啶和吡啶生物碱的生物合成等代谢途径中的代谢物表达持续下调,富集在淀粉和蔗糖代谢途径、酪氨酸代谢、植物激素的生物合成等途径中的代谢物在延迟采收8 d时表达下调。综上表明,豫中烟区烤烟上部叶延迟采收8 d为最佳采收期。本研究为确定豫中上部烟叶的适宜采收时间,科学指导田间采收提供了重要的理论依据。

关键词: 烤烟, 上部叶, 代谢组学, 氮代谢, 延迟采收