Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (12): 2681-2687.DOI: 10.11869/j.issn.100-8551.2021.12.2681
• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Next Articles
LIU Fengluan1,2(), QIN Mi1,2, YU Sheng3, LIU Qingqing1,2, ZHANG Dasheng1,2, TIAN Daike1,2,*(
)
Received:
2020-11-10
Revised:
2021-01-07
Online:
2021-12-10
Published:
2021-10-25
Contact:
TIAN Daike
刘凤栾1,2(), 秦密1,2, 宇胜3, 刘青青1,2, 张大生1,2, 田代科1,2,*(
)
通讯作者:
田代科
作者简介:
刘凤栾,男,副研究员,主要从事园林植物遗传育种及观赏性状调控机制研究。E-mail: 19liu19@163.com
基金资助:
LIU Fengluan, QIN Mi, YU Sheng, LIU Qingqing, ZHANG Dasheng, TIAN Daike. Effects of 60Co-γ Irradiation on Lotus Seeds and Morphological Analysis of a Mutant With Narrow Tepals[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(12): 2681-2687.
刘凤栾, 秦密, 宇胜, 刘青青, 张大生, 田代科. 60Co-γ射线对莲子的辐射效应及狭瓣变异株的形态分析[J]. 核农学报, 2021, 35(12): 2681-2687.
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URL: https://www.hnxb.org.cn/EN/10.11869/j.issn.100-8551.2021.12.2681
剂量 Irradiation dose/Gy | 处理莲子数 Number of irradiated seed | 萌芽数 Number of germinated seed | 萌芽率 Germination rate/% | 成活数 Number of survival seed | 存活率 Survival rate/% |
---|---|---|---|---|---|
0 (CK) | 50 | 47 | 94 | 47 | 100.0 |
20 | 50 | 46 | 92 | 46 | 100.0 |
40 | 50 | 47 | 94 | 47 | 100.0 |
80 | 50 | 47 | 94 | 47 | 100.0 |
160 | 100 | 88 | 88 | 59 | 67.0 |
320 | 100 | 90 | 90 | 35 | 38.9 |
500 | 100 | 93 | 93 | 15 | 16.1 |
750 | 200 | 154 | 77 | 1 | 0.6 |
1000 | 200 | 130 | 65 | 0 | 0.0 |
Table 1 The germination rate and survival rate of Weishan Hong seedlings under a large span of irradiation doses
剂量 Irradiation dose/Gy | 处理莲子数 Number of irradiated seed | 萌芽数 Number of germinated seed | 萌芽率 Germination rate/% | 成活数 Number of survival seed | 存活率 Survival rate/% |
---|---|---|---|---|---|
0 (CK) | 50 | 47 | 94 | 47 | 100.0 |
20 | 50 | 46 | 92 | 46 | 100.0 |
40 | 50 | 47 | 94 | 47 | 100.0 |
80 | 50 | 47 | 94 | 47 | 100.0 |
160 | 100 | 88 | 88 | 59 | 67.0 |
320 | 100 | 90 | 90 | 35 | 38.9 |
500 | 100 | 93 | 93 | 15 | 16.1 |
750 | 200 | 154 | 77 | 1 | 0.6 |
1000 | 200 | 130 | 65 | 0 | 0.0 |
Fig.2 Main morphological changes during the growth of irradiated Weishan Hong seedlings Note: A: The orthotropism of seedling petiole reduced. B: Slightly wrinkled leaves. C: Some of leaf navels displayed pink.
剂量 Irradiation dose/Gy | 微山红 Weishan Hong | 栽培品种 Cultivars | ||||||
---|---|---|---|---|---|---|---|---|
处理莲子数 Number of irradiated seed | 萌芽率 Germination rate/% | 存活率 Survival rate/% | 相对CK的 存活率 Survival rate based on CK/% | 处理莲子数 Number of irradiated seed | 萌芽率 Germination rate/% | 存活率 Survival rate/% | 相对CK的 存活率 Survival rate based on CK/% | |
0 (CK) | 20 | 90 | 100.0 | 100.0 | 100 | 91 | 90.1 | 100.0 |
150 | 50 | 92 | 60.9 | 60.9 | 100 | 84 | 47.6 | 52.8 |
160 | 50 | 90 | 62.2 | 62.2 | 100 | 87 | 48.3 | 53.6 |
170 | 50 | 92 | 52.2 | 52.2 | 100 | 81 | 46.9 | 52.1 |
180 | 50 | 92 | 52.2 | 52.2 | 100 | 89 | 42.7 | 47.3 |
200 | 50 | 94 | 48.9 | 48.9 | 100 | 85 | 40.0 | 44.3 |
Table 2 Effects of 60Co-γ irradiation on germination and seedling development of wild type and cultivars of lotus
剂量 Irradiation dose/Gy | 微山红 Weishan Hong | 栽培品种 Cultivars | ||||||
---|---|---|---|---|---|---|---|---|
处理莲子数 Number of irradiated seed | 萌芽率 Germination rate/% | 存活率 Survival rate/% | 相对CK的 存活率 Survival rate based on CK/% | 处理莲子数 Number of irradiated seed | 萌芽率 Germination rate/% | 存活率 Survival rate/% | 相对CK的 存活率 Survival rate based on CK/% | |
0 (CK) | 20 | 90 | 100.0 | 100.0 | 100 | 91 | 90.1 | 100.0 |
150 | 50 | 92 | 60.9 | 60.9 | 100 | 84 | 47.6 | 52.8 |
160 | 50 | 90 | 62.2 | 62.2 | 100 | 87 | 48.3 | 53.6 |
170 | 50 | 92 | 52.2 | 52.2 | 100 | 81 | 46.9 | 52.1 |
180 | 50 | 92 | 52.2 | 52.2 | 100 | 89 | 42.7 | 47.3 |
200 | 50 | 94 | 48.9 | 48.9 | 100 | 85 | 40.0 | 44.3 |
Fig.3 Morphological differences in flowers and leaves of Chenshan Feiyan compared to its wild type Note: A, B, C: Bud, 1 and 2 d flowers of wild Weishan Hong. D, E,F: Bud, 1 and 2 d flowers of variant Chenshan Feiyan. G, H, I:Malformed leaves of Chenshan Feiyan.
指标 Index | 辰山飞燕 Chenshan Feiyan | 微山红 Weishan Hong | ||
---|---|---|---|---|
均值 Mean value | 变化范围 Data range | 均值 Mean value | 变化范围 Data range | |
花被片宽长比 Width-length ratio of tepal | 0.31a | 0.190.45 | 0.44b | 0.310.50 |
花被片宽度 Tepal width/cm | 3.1a | 1.75.8 | 4.0a | 2.45.1 |
花被片长度 Tepal length/cm | 10.2a | 4.813.1 | 9.1a | 5.311.0 |
每朵花被片数 Tepal number per flower | 19.7a | 18.022.0 | 23.2b | 22.025.0 |
2 d花朵直径×高度 Diameter × height of 2 d flower/cm | (17.9×13.5)a | (15.520.0)×(10.516.5) | (17.6×12.4)a | (16.018.5)×(10.015.0) |
每朵雄蕊数 Stamen number per flower | 149a | 131163 | 150a | 140175 |
每朵雌蕊数 Carpel number per flower | 9.4a | 5.014.0 | 11.2a | 8.015.0 |
雄蕊长度 Stamen length/cm | 2.9a | 2.73.1 | 3.0a | 2.73.3 |
着花密度(朵/缸)Flower density per pot | 4.4a | 3.06.0 | 6.3b | 4.08.0 |
立叶密度(片/缸)Emerging leaf density per pot | 13.4a | 11.016.0 | 17.4b | 14.021.0 |
自交结实率 Selfing fruit-setting rate/% | 0.0a | - | 77.0b | 63.086.0 |
Table 3 Resistance evaluation of test-tube seedlings in Cym. Yunv
指标 Index | 辰山飞燕 Chenshan Feiyan | 微山红 Weishan Hong | ||
---|---|---|---|---|
均值 Mean value | 变化范围 Data range | 均值 Mean value | 变化范围 Data range | |
花被片宽长比 Width-length ratio of tepal | 0.31a | 0.190.45 | 0.44b | 0.310.50 |
花被片宽度 Tepal width/cm | 3.1a | 1.75.8 | 4.0a | 2.45.1 |
花被片长度 Tepal length/cm | 10.2a | 4.813.1 | 9.1a | 5.311.0 |
每朵花被片数 Tepal number per flower | 19.7a | 18.022.0 | 23.2b | 22.025.0 |
2 d花朵直径×高度 Diameter × height of 2 d flower/cm | (17.9×13.5)a | (15.520.0)×(10.516.5) | (17.6×12.4)a | (16.018.5)×(10.015.0) |
每朵雄蕊数 Stamen number per flower | 149a | 131163 | 150a | 140175 |
每朵雌蕊数 Carpel number per flower | 9.4a | 5.014.0 | 11.2a | 8.015.0 |
雄蕊长度 Stamen length/cm | 2.9a | 2.73.1 | 3.0a | 2.73.3 |
着花密度(朵/缸)Flower density per pot | 4.4a | 3.06.0 | 6.3b | 4.08.0 |
立叶密度(片/缸)Emerging leaf density per pot | 13.4a | 11.016.0 | 17.4b | 14.021.0 |
自交结实率 Selfing fruit-setting rate/% | 0.0a | - | 77.0b | 63.086.0 |
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