Effect of Bound Polyphenols in Psidium Guajava on Mice Intestinal Flora Structure and Diversity

doi:10.11869/j.issn.100-8551.2021.11.2589

Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (11): 2589-2597.DOI: 10.11869/j.issn.100-8551.2021.11.2589

• Food Irradiation·Food Science • Previous Articles Next Articles

Effect of Bound Polyphenols in Psidium Guajava on Mice Intestinal Flora Structure and Diversity

XIE Tian¹(), SUN Lin¹, FAN Wei¹, GUO Shiyin¹, XIAO Hang¹^,², TANG Zhonghai¹^,^*()

¹College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410000
²Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA

Received:2020-10-19 Accepted:2021-01-07 Online:2021-11-10 Published:2021-09-18
Contact: TANG Zhonghai

番石榴结合态多酚对小鼠肠道菌群结构及多样性的影响研究

谢甜¹(), 孙琳¹, 范伟¹, 郭时印¹, 肖航¹^,², 唐忠海¹^,^*()

¹湖南农业大学食品科学技术学院,湖南长沙 410000
²麻省大学阿莫斯特分校食品科学系,美国马萨诸塞州阿莫斯特 01003

通讯作者: 唐忠海
作者简介:谢甜,女,主要从事果蔬生物活性成分营养与功能研究。E-mail: 919383992@qq.com
基金资助:
国家自然科学基金项目(31671858);湖南省自然科学基金项目(2019JJ40114);湖南省教育厅重点项目(18A108);长沙市重点研发计划项目(kq1801016);长沙市重点研发计划项目(kq1907039)

Abstract

Abstract:

In order to explore the effects of bound polyphenols in Psidium guajava on fut microbiota in mice, CD-1 mice were divided into three groups, the blank group, the high concentration group (500 mg·kg ^-1) and the low concentration group (100 mg·kg^-1). At the end of the fifth week of gavage, the gene hypervariable V3-V4 of 16S rRNA in the gut microbiota was amplified after sterile feces collection. The alpha and beta-diversity, the species composition of the gut microbiota at the phylum and genus levels were detected by 16S rRNA gene amplicon sequencing technology. The results showed that compared to the blank group, the microbial diversity and richness in low concentration group were improved significantly (P<0.05). Bacteroides, Firmicutes, and Verrucomicrobia were the dominated gut microbiota in mice at the phylum level. At the genus level, both high and low concentrations groups could decrease the relative abundance of Bacteroides. Low concentration group could enhance the abundance of Lactobacillus, and a substantial increase of the proportion of Akkermansia was observed in high concentration group. In summary, bound polyphenols in Psidium guajava could increase diversity and richness of gut microbiota in mice, which could improve the relative abundance of probiotics, while decrease that of harmful bacteria. The results of this study would provide theoretical reference for the activity research and practical application of bound polyphenols in Psidium guajava.

Key words: Psidium guajava L., bound polyphenols, gut microbiota, 16S rRNA gene amplicon sequencing technology, diversity

摘要：

为探究番石榴结合态多酚对小鼠肠道菌群的影响,本试验将CD-1小鼠分为空白组、高浓度组(500 mg·kg^-1)和低浓度组(100 mg·kg^-1)3组,在灌胃5周后,收集小鼠的无菌粪便,扩增肠道菌群16S rRNA基因高变域V3-V4,通过16S rRNA基因扩增子测序技术检测小鼠肠道菌群的α多样性、β多样性,以及门、属水平的物种组成。结果表明,与空白组相比,低浓度组中菌群的均匀性和丰富度显著提高(P<0.05);基于门水平分析,厚壁菌门、拟杆菌门和疣微菌门3个菌门的相对丰度值较大;在属水平中,高低浓度组均能降低拟杆菌属相对丰度,低浓度组能提高乳杆菌相对丰度,并且高浓度组中艾克曼菌属的相对丰度大幅提高。综上所述,番石榴结合态多酚能提高小鼠肠道菌群的均匀性和丰富度,且对菌群结构具有调节作用,既能提高益生菌的相对丰度,也能降低有害菌的相对丰度。本研究结果可为番石榴结合态多酚的活性研究和实际应用提供理论参考。

关键词: 番石榴, 结合态多酚, 肠道菌群, 16S rRNA基因扩增子测序, 多样性

XIE Tian, SUN Lin, FAN Wei, GUO Shiyin, XIAO Hang, TANG Zhonghai. Effect of Bound Polyphenols in Psidium Guajava on Mice Intestinal Flora Structure and Diversity[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(11): 2589-2597.

谢甜, 孙琳, 范伟, 郭时印, 肖航, 唐忠海. 番石榴结合态多酚对小鼠肠道菌群结构及多样性的影响研究[J]. 核农学报, 2021, 35(11): 2589-2597.

Figures/Tables 9

Fig.1 RP-HPLC spectrum of bound polyphenols

Table 1 Changes on body weight in mice during administration of bound polyphenols(n=10)/g

组别 Group	时间Time
组别 Group	第1天	第5天	第9天	第13天	第17天	第21天	第25天	第29天
空白组 Blank	37.00±1.95	37.10±1.95	35.20±1.95	33.60±4.40	31.70±3.14	29.20±3.45	34.20±3.14	28.10±2.88
低浓度组 Low concentration	37.70±2.28	37.70±2.28	34.00±2.14	30.10±2.14	30.00±4.13	30.00±3.74	28.70±4.03	27.50±2.21
高浓度组 High concentration	37.50±5.48	37.50±5.48	33.90±5.48	31.30±7.11	30.70±7.11	28.90±6.67	30.70±7.54	27.00±7.54

Fig.2 The effect of bound polyphenols on Alpha diversity in mice Note: ‘0’ means pre-gavage, ‘1’ means mid-gavage, ‘2’ means end of gavage, ‘Blank’ means blank group, ‘Low’ means low concentration group, and ‘High’ means high concentration group. The same as following.

Table 2 Comparison of Alpha diversity indexs

组别 Group	Observed(OTU)	Chao1	ACE	Shannon	Simpson
空白组Blank	146.50±36.17	147.50±35.71	147.08±35.92	4.64±1.14	0.91±0.11
低浓度组Low concentration	154.50±43.65^*	154.50±43.58^*	154.67±43.64^*	5.03±0.54^*	0.91±0.05^*
高浓度组High concentration	136.00±16.03^*	138.40±15.54^*	137.95±15.79^*	3.68±0.81^*	0.76±0.14^*

Fig.3 The principal coordinates analysis of microbial flora in mice feces Note: The closer the distance between the two points, the smaller the difference in community composition between the two points.

Fig.4 Relative abundance of mice intestinal flora at the phylum level

Fig.5 Species relative abundance of all sample gut microbiota at the phylum level Note:The first number 1,2,3 on the abscissa represents the early, middle and end of gavaged administration respectively, and the 1 to 6, 7 to 12, 13 to 18 in the second number represents the blank group, low concentration group and high concentration group namely.

Fig.6 Species abundance cluster graph of intestinal flora at the phylum level Note:Each column in the figure represents a sample, and each row represents an OTU. The colorrepresents the read value containedin the OTU. The closer is to the red, the higher the relative abundance is.

Fig.7 Relative abundance of mice intestinal flora at thegenus level

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创刊：1987年
主管部门：农业农村部
主办单位：中国原子能农学会与中国农业科学院农产品加工研究所（前中国农业科学院原子能利用研究所）
ISSN：1000-8551

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Effect of Bound Polyphenols in Psidium Guajava on Mice Intestinal Flora Structure and Diversity

番石榴结合态多酚对小鼠肠道菌群结构及多样性的影响研究

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