Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (10): 2294-2304.DOI: 10.11869/j.issn.100-8551.2021.10.2294
• Food Irradiation·Food Science • Previous Articles Next Articles
DAI Dan1(), ZHENG Jian2,*(
), ZHOU Chengmin3, CHENG Jiyu2, DING Lizhong4,*(
)
Received:
2020-08-03
Accepted:
2020-10-04
Online:
2021-10-10
Published:
2021-08-04
Contact:
ZHENG Jian,DING Lizhong
戴丹1(), 郑剑2,*(
), 周成敏3, 成纪予2, 丁立忠4,*(
)
通讯作者:
郑剑,丁立忠
作者简介:
戴丹,女,副教授,主要从事农产品冷链物流、贮藏运销及生鲜农产品电子商务研究。E-mail: boatdriver@163.com
基金资助:
DAI Dan, ZHENG Jian, ZHOU Chengmin, CHENG Jiyu, DING Lizhong. Effects of Oxalic Acid on Edible Quality of Bamboo Shoots (Bambusa oldhami) Without Sheaths During Cold Storage and the Related Mechanism[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(10): 2294-2304.
戴丹, 郑剑, 周成敏, 成纪予, 丁立忠. 草酸对冷藏去壳马蹄笋的保鲜效果及机制研究[J]. 核农学报, 2021, 35(10): 2294-2304.
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URL: https://www.hnxb.org.cn/EN/10.11869/j.issn.100-8551.2021.10.2294
物种名称 Species | 基因名称 Gene | 基因序列号 Accession | 引物序列 Primer sequences | 扩增长度 Size/bp | 退火温度 Annealing/℃ |
---|---|---|---|---|---|
毛竹Phyllostachys edulis | Actin | FJ601918.1 | F: TGCCCTTGATTATGAGCAGG R: AACCTTTCTGCTCCGATGGT | 108 | 60 |
毛竹Phyllostachys edulis | cinnamyl alcohol dehydrogenase(CAD) | EF549577.1 | F: GGTCACCGTGATCAGCTCGT R: TTACCTTCATTTGCTCGGCG | 104 | 60 |
毛竹Phyllostachys edulis | polyphenol oxidase (PPO) | PH01000032G1250 | F: ATAGCGCTGATGAAGGCGAT R: TGGATCTGCACGTTCAGCTC | 132 | 60 |
毛竹Phyllostachys edulis | peroxidase (POD) | FP092331.1 | F: GGCATGATCCACCAGCTCAC R: TTCCTCCTGATCTCCCCCTG | 116 | 60 |
高节竹Phyllostachys prominens | Phenylalanine ammonia-lyase (PAL1) | AY450643.2 | F: CTCCGTGTTTGCCAAGGTTG R: GAGCGGCCCTCAGTGATTCT | 132 | 60 |
毛竹Phyllostachys edulis | superoxide dismutase (SOD) | PH01002962G0030 | F: GCCACCTACGTCGCCAACTA R: GAACTTGATGGCGCTCTGGA | 104 | 60 |
慈竹Bambusa emeiensis | catalase(CAT) | PH01000019G1840 | F: CATGCATGCGTTTGGATTTG R: CAGAATAGCCGCTTTGAGCG | 102 | 60 |
Table 1 Real-time PCR primers and conditions
物种名称 Species | 基因名称 Gene | 基因序列号 Accession | 引物序列 Primer sequences | 扩增长度 Size/bp | 退火温度 Annealing/℃ |
---|---|---|---|---|---|
毛竹Phyllostachys edulis | Actin | FJ601918.1 | F: TGCCCTTGATTATGAGCAGG R: AACCTTTCTGCTCCGATGGT | 108 | 60 |
毛竹Phyllostachys edulis | cinnamyl alcohol dehydrogenase(CAD) | EF549577.1 | F: GGTCACCGTGATCAGCTCGT R: TTACCTTCATTTGCTCGGCG | 104 | 60 |
毛竹Phyllostachys edulis | polyphenol oxidase (PPO) | PH01000032G1250 | F: ATAGCGCTGATGAAGGCGAT R: TGGATCTGCACGTTCAGCTC | 132 | 60 |
毛竹Phyllostachys edulis | peroxidase (POD) | FP092331.1 | F: GGCATGATCCACCAGCTCAC R: TTCCTCCTGATCTCCCCCTG | 116 | 60 |
高节竹Phyllostachys prominens | Phenylalanine ammonia-lyase (PAL1) | AY450643.2 | F: CTCCGTGTTTGCCAAGGTTG R: GAGCGGCCCTCAGTGATTCT | 132 | 60 |
毛竹Phyllostachys edulis | superoxide dismutase (SOD) | PH01002962G0030 | F: GCCACCTACGTCGCCAACTA R: GAACTTGATGGCGCTCTGGA | 104 | 60 |
慈竹Bambusa emeiensis | catalase(CAT) | PH01000019G1840 | F: CATGCATGCGTTTGGATTTG R: CAGAATAGCCGCTTTGAGCG | 102 | 60 |
贮藏时间 Storage time/d | 草酸浓度 Oxalic acid concentration /(mmol·L-1) | 硬度 Firmness/N | L*值 L* value | 木质素含量 Ligin content/% | 失重率 Weight loss/% |
---|---|---|---|---|---|
0 | 8.40±1.46c | 86.77±1.39a | 0.2±0.08c | 0c | |
10 | 0(清水) | 27.51±1.50a | 64.07±2.76d | 1.33±0.09a | 2.42±0.16a |
5 | 20.96±2.10b | 79.43±2.12b | 0.90±0.09b | 1.68±0.11b | |
10 | 22.64±2.86b | 73.53±2.26c | 0.97±0.06b | 1.60±0.13b | |
15 | 19.88±2.22b | 66.23±2.70d | 0.93±0.11b | 1.73±0.11b | |
20 | 20.55±3.14b | 65.53±1.27d | 0.92±0.11b | 1.67±0.17b |
Table 2 Effects of different oxalic acid treatment on firmness, L*value, lignin contents and weight loss of bamboo shoot during storage at 6℃ for 10 days
贮藏时间 Storage time/d | 草酸浓度 Oxalic acid concentration /(mmol·L-1) | 硬度 Firmness/N | L*值 L* value | 木质素含量 Ligin content/% | 失重率 Weight loss/% |
---|---|---|---|---|---|
0 | 8.40±1.46c | 86.77±1.39a | 0.2±0.08c | 0c | |
10 | 0(清水) | 27.51±1.50a | 64.07±2.76d | 1.33±0.09a | 2.42±0.16a |
5 | 20.96±2.10b | 79.43±2.12b | 0.90±0.09b | 1.68±0.11b | |
10 | 22.64±2.86b | 73.53±2.26c | 0.97±0.06b | 1.60±0.13b | |
15 | 19.88±2.22b | 66.23±2.70d | 0.93±0.11b | 1.73±0.11b | |
20 | 20.55±3.14b | 65.53±1.27d | 0.92±0.11b | 1.67±0.17b |
Fig.1 Effects of oxalic acid treatment on respiration rate(A) and L*value(B) of bamboo shoots during storage at 6℃ Note:Different lowercase letters indicate significant difference at 0.05 level among different treatments at the same stroage time. The same as following.
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