Effect of Humidity on Wound Healing of Sweet Potato

doi:10.11869/j.issn.100-8551.2021.09.2111

Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (9): 2111-2117.DOI: 10.11869/j.issn.100-8551.2021.09.2111

• Food Irradiation·Food Science • Previous Articles Next Articles

Effect of Humidity on Wound Healing of Sweet Potato

WEI Lei¹(), LIU Kaimeng¹, DONG Zhongfu¹, XU Jingyi¹, WU Yijiao¹, XIONG Chunyu¹, SUN Jie², WANG Caixia¹^,^*()

¹ College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014
² Academy of Agricultural Planning and Engineering, Ministry of Agriculture and Rural Affairs, Beijing 100125

Received:2020-06-05 Accepted:2020-10-27 Online:2021-09-10 Published:2021-07-22
Contact: WANG Caixia

湿度对甘薯愈伤的影响

魏蕾¹(), 刘开梦¹, 董中富¹, 徐静嶷¹, 吴怡娇¹, 熊春雨¹, 孙洁², 王彩霞¹^,^*()

¹ 四川农业大学食品学院,四川雅安 625014
² 农业农村部规划设计研究院,北京 100125

通讯作者: 王彩霞
作者简介:魏蕾,女,主要从事食品加工与贮藏技术研究。E-mail : 810244624@qq.com
基金资助:
农业部农产品产后处理重点实验室开放课题项目(KLAPPH2-2017-06);国家重点研发计划(2017YFD0401305);四川农业大学科研兴趣培养项目(2020604)

Abstract

Abstract:

The Pushu32 sweet potato was used as material to explore the effect of postharvest humidity on the wound healing of sweet potato. After an artificially mechanical damage, the sweet potato was stored at 25℃ under different humidity(RH50% and RH80%). The lignin accumulation, weight loss rate, respiratory intensity, key enzyme activity, total phenol and flavonoid content during the healing process of the sweet potato were measured to evaluate the effect of humidity on the wound healing of sweet potato. The results showed that RH80% enhanced the formation of healing tissues for mechanical damaged sweet potato than RH50%; lignin accumulation peaked after 4d of healing. Compared with RH50%, RH80% treatment could significantly increase the activity of phenylalanine ammonialyase(PAL), peroxidase(POD) and polyphenol oxidase(PPO)(P<0.05), elevate the content of total phenols and flavonoids, bring-forwards the respiration peak and reduce the weight loss of the sweet potato. The correlation analysis revealed a significant correlation between lignin content with PAL, POD, PPO activity, total phenol and flavonoid content under RH80% healing treatment(P<0.05). This study provides the theoretical support to the wound healing of postharvest sweet potato.

Key words: sweet potato, humidity, wound healing

摘要：

为探索湿度对甘薯愈伤的影响,本试验以普薯32号甘薯为原料,人工模拟机械损伤后于25℃、不同相对湿度(RH50%和RH80%)条件下愈伤,通过比较甘薯木质素生成、失重率、呼吸强度、愈伤过程关键酶活性、总酚和类黄酮含量的差异,评价湿度对甘薯愈伤的影响。结果表明,RH80%条件下较RH50%更有利于甘薯愈伤木栓组织的形成,在愈伤4 d时甘薯木质素积累达到最高。与RH50%组相比,RH80%条件处理能显著提高甘薯愈伤组织苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)和多酚氧化酶(PPO)活性(P<0.05),提升总酚、类黄酮含量,促进呼吸高峰提前,降低甘薯重量损失。相关性分析表明,RH80%条件下愈伤,甘薯木质素含量与PAL、POD、PPO活性及总酚、类黄酮含量呈显著正相关(P<0.05)。本研究结果为甘薯采后愈伤提供了一定的理论依据。

关键词: 甘薯, 湿度, 愈伤

WEI Lei, LIU Kaimeng, DONG Zhongfu, XU Jingyi, WU Yijiao, XIONG Chunyu, SUN Jie, WANG Caixia. Effect of Humidity on Wound Healing of Sweet Potato[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(9): 2111-2117.

魏蕾, 刘开梦, 董中富, 徐静嶷, 吴怡娇, 熊春雨, 孙洁, 王彩霞. 湿度对甘薯愈伤的影响[J]. 核农学报, 2021, 35(9): 2111-2117.

Figures/Tables 8

Fig.1 Effect of humidity on lignin content of sweet potato Note:* indicates significant difference between different groups in the same callus time at 0.05 level. The same as following.

Fig.2 Effect of humidity on lignin accumulation of sweet potato

Fig.3 Effect of humidity on weight loss rate of sweet potato

Fig.4 The effect of humidity on the respiration of sweet potato

Fig.5 Effect of humidity on enzyme activity of sweet potato

Fig.6 Effect of humidity on total phenol content of sweet potato

Fig.7 Effect of humidity on flavonoids content of sweet potato

Table 1 Correlation among lignin content, enzyme activity and metabolites of RH80% sweet potato

指标 Index	木质素含量 Lignin content	PAL活性 PAL activity	POD活性 POD activity	PPO活性 PPO activity	总酚含量 Total phenol content	类黄酮含量 Flavonoid content
木质素含量 Lignin content	1	0.900^*	0.942^*	0.924^*	0.899^*	0.946^*
PAL活性 PAL activity		1	0.782	0.995^**	0.660	0.780
POD活性 POD activity			1	0.817	0.910^*	0.915^*
PPO活性 PPO activity				1	0.724	0.835
总酚含量 Total phenol content					1	0.982^**
类黄酮含量 Flavonoid content						1

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创刊：1987年
主管部门：农业农村部
主办单位：中国原子能农学会与中国农业科学院农产品加工研究所（前中国农业科学院原子能利用研究所）
ISSN：1000-8551

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Effect of Humidity on Wound Healing of Sweet Potato

湿度对甘薯愈伤的影响

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