Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (9): 2094-2101.DOI: 10.11869/j.issn.100-8551.2021.09.2094

• Food Irradiation·Food Science • Previous Articles     Next Articles

Ultrahigh Pressure Extraction of Polygonatum sibiricum Polysaccharide and Mechanism of Improving Exercise Endurance

ZHANG Shikai(), WANG Min, CHENG Xinxin, ZHANG Qiyue, WU Peng1,*()   

  1. College of Food Science and Engineering/Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, Shandong Agricultural University, Tai'an, Shandong 271018
  • Received:2020-06-09 Accepted:2020-10-04 Online:2021-09-10 Published:2021-07-22
  • Contact: WU Peng

超高压提取黄精多糖及提高运动耐力机制

张士凯(), 王敏, 程欣欣, 张启月, 吴澎1,*()   

  1. 山东农业大学食品科学与工程学院/山东省高校食品加工技术与质量控制重点实验室,山东 泰安 271018
  • 通讯作者: 吴澎
  • 作者简介:张士凯,男,主要从事食品科学研究。E-mail: zskcxx@163.com
  • 基金资助:
    泰安市科技发展计划(49557040-x);山东农业大学作物生物学国家重点实验室开放课题基金(2015KF14);山东省重点研发计划(2017GNC10101)

Abstract:

In order to determine the optimal extraction process of polygonatum polysaccharide (PPP), Polygonatum was used as the raw material using ultra-high pressure technology after processed. The effect of pressure strength, pressure holding time, solid-liquid ratio on PPP extraction rate were analyzed. Box-Behnken response surface analysis (RSM) was used to optimize the PPP extraction conditions, and the obtained polysaccharides were tested in vivo to analyze the changes in the content of related enzymes in serum, liver and left leg skeletal muscle in mice. The mechanism of improving exercise endurance was investigated. The results showed that the optimal extraction process conditions for PPP were: liquid-material ratio 23 pressure holding time 6.73 min, pressure intensity 293 MPa. Under these conditions, the PPP yield was 13.46%; which could significantly improve the exhausted swimming time of mice (P<0.05). Compared with the swimming control group, the contents of SUN, Lac and MDA in mice were significantly decreased (P<0.05), At the same time lactate dehydrogenase (LDH) decreased, ATPase and CK increased, which had a better effect of improving exercise endurance, and provided data support for the mechanism of PPP to improve exercise endurance.

Key words: response surface optimization, polygonatum polysaccharide, ultra high pressure, exercise endurance, stress injuries

摘要:

为确定炮制黄精多糖(PPP)的最佳提取工艺,本试验以黄精为原料,进行炮制,利用超高压技术提取PPP,分析了压力强度、保压时间、料液比对PPP提取率的影响,采用Box-Behnken响应面分析法(RSM)优化PPP提取条件,并对所得多糖进行动物体内试验,分析小鼠血清、肝脏和左腿腓肠肌中相关酶的活性变化,探究其提高机体运动耐力机制。结果表明,PPP最佳提取工艺条件为:液料比23 mL·g-1、保压时间6.73 min,压力强度293 MPa,在此条件下PPP得率为13.46%;能显著提高小鼠力竭游泳时间(P<0.05),相比于游泳对照组,小鼠血清中尿素氮(SUN)、乳酸(Lac)和肝脏丙二醛(MDA)含量显著下降(P<0.05),同时乳酸脱氢酶(LDH)活性下降、Ca2+Mg2+-ATP酶及肌酸激酶(CK)活性上升,对提高小鼠运动耐力具有较好的效果。本研究结果为超高压提取PPP奠定了基础,并为PPP提高运动耐力的机制研究提供了数据支持。

关键词: 响应面优化, 黄精多糖, 超高压, 运动耐力, 应激损伤