Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (9): 2016-2026.DOI: 10.11869/j.issn.100-8551.2021.09.2016

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles     Next Articles

Geneome-Wide Identification and Expression Analysis of Auxin Response Factor Gene Family in Maize

CAO Liru1(), ZHANG Qianjin1, GUO Zining2, LU Xiaomin1, ZHANG Xin1, WEI Xin1, HUANG-FU Baishu3,*(), WANG Zhenhua1,*()   

  1. 1 Grain Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002
    2 Science and Technology Achievement Demonstration and Promotion Division, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002
    3 Jiaozuo Academy of Agriculture and Forestry Sciences, Jiaozuo, Henan 454150
  • Received:2020-06-28 Accepted:2020-10-04 Online:2021-09-10 Published:2021-07-22
  • Contact: HUANG-FU Baishu,WANG Zhenhua


曹丽茹1(), 张前进1, 郭子宁2, 鲁晓民1, 张新1, 魏昕1, 皇甫柏树3,*(), 王振华1,*()   

  1. 1 河南省农业科学院粮食作物研究所,河南 郑州 450002
    2 河南省农业科学院科技成果示范推广处, 河南 郑州 450002
    3 焦作市农林科学研究院,河南 焦作 454150
  • 通讯作者: 皇甫柏树,王振华
  • 作者简介:曹丽茹,女,主要从事玉米遗传育种及生物信息学分析。E-mail:
  • 基金资助:


Auxin response factor (ARF) can specifically bind to the promoter region of response gene to regulate plant growth and development. In order to understand furtherly the number, basic characteristics, evolutionary relationship of ARF gene family in maize and the expression pattern of ARF in response to abiotic stress and hormone. In this study, the ARF gene family in maize was identified by bioinformatics, and the physiochemical properties and the phylogeny of proteins were analyzed. RT-qPCR was used to analyze the temporal and spatial expression patterns and their expression under high temperature, drought, salt and ABA treatment of fourZmARFs. The results showed that 36 ARFs genes were randomly and unevenly distributed on 9 chromosomes, and the amino acid length, molecular weight, isoelectric point and secondary structure of the encoding protein were quite different. The protein phylogenetic tree of sorghum, rice, Arabidopsis and maize showed that these ARF proteins are divided into four categories Ⅰ~Ⅳ. Maize ARF has the closest genetic relationship with sorghum ARF, and the farthest genetic relationship with Arabidopsis ARF, and there were intragenic and intergenic duplication occurs in maize.The cis-acting elements of promoter are main elements in response to drought, low temperature, oxidation and hormones. RT-qPCR results showed that the expression of ZmARF1,ZmARF6,ZmARF13 and ZmARF22 were higher in the tassel branches and embryos, but lower in the pollen. The four genes were significantly up-regulated when induced by high temperature, drought, salt and ABA, except that ZmARF22 gene had decreased expression after high temperature treatment. Subcellular localization indicated that the proteins encoded byZmARF1, ZmARF6, ZmARF13 and ZmARF22 are localized in the nucleus. The results of this study provide a theoretical basis for revealing the function of maize ARF protein and mining stress resistant genes, and also provide molecular resources for stress resistant breeding.

Key words: maize, ZmARFs, bioinformatics, expression pattern, cell nucleus



关键词: 玉米, ZmARFs, 生物信息学, 表达模式, 细胞核