The Detection of QTL Confers Cold Tolerance at the Bud Bursting Period in Rice Using CSSLs Population

doi:10.11869/j.issn.100-8551.2021.09.1971

Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (9): 1971-1978.DOI: 10.11869/j.issn.100-8551.2021.09.1971

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles Next Articles

The Detection of QTL Confers Cold Tolerance at the Bud Bursting Period in Rice Using CSSLs Population

DENG Haodong¹(), YU Meixia¹(), WU Guangliang¹, LUO Xin¹, SONG Guiting¹, CHEN Liping¹, HE Haohua¹^,²^,³, BIAN Jianmin¹^,²^,³^,^*()

¹ Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Nanchang, Jiangxi 330045
² Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Jiangxi Province, Nanchang, Jiangxi 330045
³ Jiangxi Super Rice Engineering Technology Research Center, Nanchang, Jiangxi 330045

Received:2020-02-18 Accepted:2020-04-01 Online:2021-09-10 Published:2021-07-22
Contact: BIAN Jianmin

利用染色体片段置换系群体定位水稻芽期耐低温主效QTL

邓浩东¹(), 余镁霞¹(), 吴光亮¹, 罗鑫¹, 宋贵庭¹, 陈利平¹, 贺浩华¹^,²^,³, 边建民¹^,²^,³^,^*()

¹ 作物生理生态与遗传育种教育部重点实验室,江西南昌 330045
² 作物生理生态与遗传育种江西省重点实验室,江西南昌 330045
³ 江西省水稻高水平工程研究中心,江西南昌 330045

通讯作者: 边建民
作者简介:邓浩东,男,主要从事水稻遗传育种研究。E-mail: 1303051137@qq.com
余镁霞,女,主要从事水稻遗传育种研究。E-mail: 1873831573@qq.com。第一联系人：^**同为第一作者。
基金资助:
江西省杰出青年人才资助计划项目(20192BCB23010);江西省教育厅科技研究项目(GJJ170241);国家大学生创新创业项目(201910410003)

Abstract

Abstract:

Rice is one of the most important food crops in the world. In order to explore the genes associated with low temperature to lerant in rice at the bud bursting period, the chromosome segment substitution lines (CSSLs) derived 9311 (recipient)/Nipponbare (donor) was used to study the rice cold tolerance. The CSSLs was treated at low temperatures of 7℃ and 15℃ for 7 days after the emerge of rice bud respectively, and then recovered at 28℃ for 3 days. After the treatments, the QTL associated with seed survival rate were detected. Under low temperature treatment at 7℃, two major QTLs, qCS7T10 and qCS7T11 on chromosome 10 and 11, affecting seed survival rate, were detected, with LOD values of 7.26 and 5.87, and contribution of 18.85% and 14.92%, respectively. Under 15℃ low temperature treatment, one major QTL, qCS15T5, was detected. qCS15T5 was located on chromosome 5 of rice, LOD value was 7.61, and the contribution rate was 25.69%. The results showed that the QTLs controlling seed survival rate under different low temperature reatments at the bud bursting period were different. It has laid a certain foundation for future breeding and pyorarniding of more low temperature tolerance QTLs to improve the adaptability of rice to different low temperatures and reduce the impact of low temperature stress on rice bud stage.

Key words: rice, bud bursting period, QTL, cold stress, the chromosome segment substitutin lines(CSSLs)

摘要：

水稻是世界上重要的粮食作物之一,低温胁迫影响水稻芽期生长,导致减产。为挖掘影响水稻芽期耐冷的基因,本研究以9311(受体)/日本晴(供体)染色体片段置换系群体为材料,将萌发的种子分别在7℃和15℃低温条件下处理7 d,再在28℃恢复生长3 d,测定种子存活率并定位其中影响芽期耐低温数量性状位点(QTL)。7℃低温胁迫下,共检测到2个芽期耐低温主效QTL:qCS7T10和qCS7T11;两者分别位于水稻第10和第11号染色体上,LOD值分别为7.26和5.87,贡献率分别为18.85%和14.92%。15℃低温胁迫下,共检测到1个芽期耐低温主效QTL:qCS15T5,其位于水稻第5号染色体上,LOD值为7.61,贡献率为25.69%。结果表明不同低温处理下,控制水稻芽期耐低温QTL不同。本研究为今后育种聚合更多的耐低温QTL来提高水稻对不同低温的适应能力,降低低温胁迫对水稻芽期的影响奠定了一定的基础。

关键词: 水稻, 芽期, QTL, 低温处理, 染色体片段置换系

DENG Haodong, YU Meixia, WU Guangliang, LUO Xin, SONG Guiting, CHEN Liping, HE Haohua, BIAN Jianmin. The Detection of QTL Confers Cold Tolerance at the Bud Bursting Period in Rice Using CSSLs Population[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(9): 1971-1978.

邓浩东, 余镁霞, 吴光亮, 罗鑫, 宋贵庭, 陈利平, 贺浩华, 边建民. 利用染色体片段置换系群体定位水稻芽期耐低温主效QTL[J]. 核农学报, 2021, 35(9): 1971-1978.

Figures/Tables 6

References 37

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创刊：1987年
主管部门：农业农村部
主办单位：中国原子能农学会与中国农业科学院农产品加工研究所（前中国农业科学院原子能利用研究所）
ISSN：1000-8551

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染色体 Chromosome	标记数目 Number of marker	标记间平均距离 Mean distance between adjacent bins/Mb	标记间最小距离 Min distance between adjacent bins/Mb	标记间最大距离 Max distance between adjacent bins/Mb
1	68	0.64	0.10	3.40
2	64	0.56	0.10	2.90
3	59	0.62	0.10	3.45
4	69	0.52	0.10	4.70
5	47	0.64	0.10	3.60
6	37	0.82	0.10	12.85
7	52	0.57	0.10	2.30
8	34	0.84	0.15	8.90
9	33	0.68	0.15	1.85
10	59	0.40	0.10	1.00
11	76	0.38	0.10	2.50
12	57	0.47	0.10	2.95

染色体 Chromosome	标记数目 Number of marker	标记间平均距离 Mean distance between adjacent bins/Mb	标记间最小距离 Min distance between adjacent bins/Mb	标记间最大距离 Max distance between adjacent bins/Mb
1	68	0.64	0.10	3.40
2	64	0.56	0.10	2.90
3	59	0.62	0.10	3.45
4	69	0.52	0.10	4.70
5	47	0.64	0.10	3.60
6	37	0.82	0.10	12.85
7	52	0.57	0.10	2.30
8	34	0.84	0.15	8.90
9	33	0.68	0.15	1.85
10	59	0.40	0.10	1.00
11	76	0.38	0.10	2.50
12	57	0.47	0.10	2.95

处理 Treatment	亲本 Parents		CSSLs群体 CSSLs population
处理 Treatment	9311	日本晴 Nipponbare	均值 Mean	标准误 SE	偏度 Skewness	峰度 Kurtosis	最小值 Minimum	最大值 Maximum
7℃	51.88%	97.78%	58%	0.28	-1.14	0.11	0%	94%
15℃	96.67%	95.56%	93%	0.06	-1.28	1.78	71%	100%

处理 Treatment	亲本 Parents		CSSLs群体 CSSLs population
处理 Treatment	9311	日本晴 Nipponbare	均值 Mean	标准误 SE	偏度 Skewness	峰度 Kurtosis	最小值 Minimum	最大值 Maximum
7℃	51.88%	97.78%	58%	0.28	-1.14	0.11	0%	94%
15℃	96.67%	95.56%	93%	0.06	-1.28	1.78	71%	100%

The Detection of QTL Confers Cold Tolerance at the Bud Bursting Period in Rice Using CSSLs Population

利用染色体片段置换系群体定位水稻芽期耐低温主效QTL

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References 37

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处理 Treatment	QTL	连锁标记 Linked marker	LOD值 LOD score	贡献率 PVE/%	加性效应 Addtive
7℃	qCS7T10	Chr10-bin475	7.26	18.85	-0.22
7℃	qCS7T11	Chr11-bin590	5.87	14.92	-0.19
15℃	qCS15T5	Chr5-bin306	7.61	25.69	-0.08

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