Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (9): 1971-1978.DOI: 10.11869/j.issn.100-8551.2021.09.1971

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles     Next Articles

The Detection of QTL Confers Cold Tolerance at the Bud Bursting Period in Rice Using CSSLs Population

DENG Haodong1(), YU Meixia1(), WU Guangliang1, LUO Xin1, SONG Guiting1, CHEN Liping1, HE Haohua1,2,3, BIAN Jianmin1,2,3,*()   

  1. 1 Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Nanchang, Jiangxi 330045
    2 Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Jiangxi Province, Nanchang, Jiangxi 330045
    3 Jiangxi Super Rice Engineering Technology Research Center, Nanchang, Jiangxi 330045
  • Received:2020-02-18 Accepted:2020-04-01 Online:2021-09-10 Published:2021-07-22
  • Contact: BIAN Jianmin

利用染色体片段置换系群体定位水稻芽期耐低温主效QTL

邓浩东1(), 余镁霞1(), 吴光亮1, 罗鑫1, 宋贵庭1, 陈利平1, 贺浩华1,2,3, 边建民1,2,3,*()   

  1. 1 作物生理生态与遗传育种教育部重点实验室,江西 南昌 330045
    2 作物生理生态与遗传育种江西省重点实验室,江西 南昌 330045
    3 江西省水稻高水平工程研究中心,江西 南昌 330045
  • 通讯作者: 边建民
  • 作者简介:邓浩东,男,主要从事水稻遗传育种研究。E-mail: 1303051137@qq.com
    余镁霞,女,主要从事水稻遗传育种研究。E-mail: 1873831573@qq.com第一联系人:**同为第一作者。
  • 基金资助:
    江西省杰出青年人才资助计划项目(20192BCB23010);江西省教育厅科技研究项目(GJJ170241);国家大学生创新创业项目(201910410003)

Abstract:

Rice is one of the most important food crops in the world. In order to explore the genes associated with low temperature to lerant in rice at the bud bursting period, the chromosome segment substitution lines (CSSLs) derived 9311 (recipient)/Nipponbare (donor) was used to study the rice cold tolerance. The CSSLs was treated at low temperatures of 7℃ and 15℃ for 7 days after the emerge of rice bud respectively, and then recovered at 28℃ for 3 days. After the treatments, the QTL associated with seed survival rate were detected. Under low temperature treatment at 7℃, two major QTLs, qCS7T10 and qCS7T11 on chromosome 10 and 11, affecting seed survival rate, were detected, with LOD values of 7.26 and 5.87, and contribution of 18.85% and 14.92%, respectively. Under 15℃ low temperature treatment, one major QTL, qCS15T5, was detected. qCS15T5 was located on chromosome 5 of rice, LOD value was 7.61, and the contribution rate was 25.69%. The results showed that the QTLs controlling seed survival rate under different low temperature reatments at the bud bursting period were different. It has laid a certain foundation for future breeding and pyorarniding of more low temperature tolerance QTLs to improve the adaptability of rice to different low temperatures and reduce the impact of low temperature stress on rice bud stage.

Key words: rice, bud bursting period, QTL, cold stress, the chromosome segment substitutin lines(CSSLs)

摘要:

水稻是世界上重要的粮食作物之一,低温胁迫影响水稻芽期生长,导致减产。为挖掘影响水稻芽期耐冷的基因,本研究以9311(受体)/日本晴(供体)染色体片段置换系群体为材料,将萌发的种子分别在7℃和15℃低温条件下处理7 d,再在28℃恢复生长3 d,测定种子存活率并定位其中影响芽期耐低温数量性状位点(QTL)。7℃低温胁迫下,共检测到2个芽期耐低温主效QTL:qCS7T10和qCS7T11;两者分别位于水稻第10和第11号染色体上,LOD值分别为7.26和5.87,贡献率分别为18.85%和14.92%。15℃低温胁迫下,共检测到1个芽期耐低温主效QTL:qCS15T5,其位于水稻第5号染色体上,LOD值为7.61,贡献率为25.69%。结果表明不同低温处理下,控制水稻芽期耐低温QTL不同。本研究为今后育种聚合更多的耐低温QTL来提高水稻对不同低温的适应能力,降低低温胁迫对水稻芽期的影响奠定了一定的基础。

关键词: 水稻, 芽期, QTL, 低温处理, 染色体片段置换系