Study on Photosynthetic Characteristics of Foxtail Millet Mutant With Yellow Leaf Colour

doi:10.11869/j.issn.100-8551.2021.09.1964

Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (9): 1964-1970.DOI: 10.11869/j.issn.100-8551.2021.09.1964

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles Next Articles

Study on Photosynthetic Characteristics of Foxtail Millet Mutant With Yellow Leaf Colour

LI Junxia(), QIN Na^*(), ZHU Cancan, WANG Chunyi, DAI Shutao, SONG Yinghui, CHEN Yuxiang

Cereal Crops Institute, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002

Received:2020-06-08 Accepted:2020-07-14 Online:2021-09-10 Published:2021-07-22
Contact: QIN Na

谷子黄叶色突变体光合特性研究

李君霞(), 秦娜^*(), 朱灿灿, 王春义, 代书桃, 宋迎辉, 陈宇翔

河南省农业科学院粮食作物研究所,河南郑州 450002

通讯作者: 秦娜
作者简介:李君霞,女,副研究员,主要从事谷子遗传育种及高效栽培技术研究。E-mail: lijunxia@126.com
基金资助:
国家现代农业产业技术体系专项资金项目(nycytx-CARS-06);河南省科技攻关项目(192102110136)

Abstract

Abstract:

To explore the mechanism of high photosynthesis efficiency of millet mutant with yellow leaf colour. The photosynthetic pigment, photosynthetic rate, stomatal traits, fluorescence parameters,activity of key photosynthetic enzymes and yield characteristics of two yellow leaf colour mutant lines ylm-1 and ylm-2 were studied, the wild type of “Yugu 1” was used as the control. The results suggested that the contents of photosynthetic pigment in mutants were 50%~60% of that in Yugu1. The photosynthetic rates of mutants were higher than that of the control under high light intensity and had no obvious “Midday depression phenomenon”. The stomatal conductance of ylm-1 and ylm-2 were increased significantly. The fluorescence parameters suggested that light energy efficiency and conversion efficiency of mutants were obviously higher than that of the wild type. The activity of key photosynthetic enzyme ribulose-1,5-bisphosphate carboxylase (Rubisco) in mutants were significantly lower than that in control, but the activities of phosphoenolpyruvate carboxylase (PEPCase) and NADP-malic enzyme (NADP-ME) were 23.3%, 24.4% and 12.9%,15.5% higher than those of the control, respectively. The results of yield characteristics showed that the weight per spike, seed weight per spike and thousand grain weight were increased significantly compared with control. These results provide an important theoretical basis for high light efficiency and molecular marker assisted selection breeding in millet.

Key words: yellow leaf colour mutant, photosynthetic characteristics, stomatal conductance, fluorescence parameters, yield traits

摘要：

为解析谷子黄叶色突变体高光合特性机理,以野生型豫谷1号为对照,分析了谷子全生育期黄叶色突变体ylm-1和ylm-2的光合色素、光合速率、气孔特性、荧光参数、光合关键酶活性和产量性状。结果表明,突变体光合色素含量为野生型豫谷1号的50%~60%,强光下突变体光合速率(Pn)较野生型显著提高,且无明显的“午休睡眠现象”;ylm-1和ylm-2气孔导度(Gs)显著增加。荧光参数结果显示,与野生型相比,突变体的光能利用效率和转化效率明显增加,光合关键酶1,5二磷酸核酮糖羧化酶(Rubisco)活性明显降低,而ylm-1和ylm-2磷酸烯醇式丙酮酸羧化酶(PEPCase)和苹果酸酶(NADP-ME)活性分别增加了23.3%、24.4%和12.9%、15.5%。产量相关性状分析表明,突变体单穗质量、穗粒质量及千粒质量较野生型显著增加。本研究结果为谷子高光效及分子标记辅助选择育种提供了重要的理论依据。

关键词: 黄叶色突变体, 光合特性, 气孔导度, 荧光参数, 产量性状

LI Junxia, QIN Na, ZHU Cancan, WANG Chunyi, DAI Shutao, SONG Yinghui, CHEN Yuxiang. Study on Photosynthetic Characteristics of Foxtail Millet Mutant With Yellow Leaf Colour[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(9): 1964-1970.

李君霞, 秦娜, 朱灿灿, 王春义, 代书桃, 宋迎辉, 陈宇翔. 谷子黄叶色突变体光合特性研究[J]. 核农学报, 2021, 35(9): 1964-1970.

Figures/Tables 8

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创刊：1987年
主管部门：农业农村部
主办单位：中国原子能农学会与中国农业科学院农产品加工研究所（前中国农业科学院原子能利用研究所）
ISSN：1000-8551

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杂交组合 Hybrid combination	F₂总株数 Total number of F₂ population	正常株数 Number of normal lines	黄色株数 Number of yellow lines	分离比 Segregation ratio	χ²检验χ² test
					χ²	P
					ylm-1/豫谷1号 ylm-1/Yugu 1	536	416	120	3.4∶1	1.81	0.10~0.25
ylm-2/保谷22 ylm-2/Baogu 22	572	448	124	3.6∶1	3.19	0.05~0.10

杂交组合 Hybrid combination	F₂总株数 Total number of F₂ population	正常株数 Number of normal lines	黄色株数 Number of yellow lines	分离比 Segregation ratio	χ²检验χ² test
					χ²	P
					ylm-1/豫谷1号 ylm-1/Yugu 1	536	416	120	3.4∶1	1.81	0.10~0.25
ylm-2/保谷22 ylm-2/Baogu 22	572	448	124	3.6∶1	3.19	0.05~0.10

株系 Lines	叶绿素a含量 Chlorophyll a content /(mg·g^-1)	叶绿素b含量 Chlorophyll b content /(mg·g^-1)	类胡萝卜素含量 Carotenoids content /(mg·g^-1)	光合色素含量 Photosynthetic pigment content/(mg·g^-1)	类胡萝卜素/总光合色素 Carotenoids/ total photosynthetic pigment
ylm-1	0.87b	0.66b	0.49b	1.54b	0.31a
ylm-2	0.88b	0.56b	0.46b	1.44b	0.32a
豫谷1号Yugu 1	1.21a	1.41a	0.83a	2.62a	0.32a

株系 Lines	叶绿素a含量 Chlorophyll a content /(mg·g^-1)	叶绿素b含量 Chlorophyll b content /(mg·g^-1)	类胡萝卜素含量 Carotenoids content /(mg·g^-1)	光合色素含量 Photosynthetic pigment content/(mg·g^-1)	类胡萝卜素/总光合色素 Carotenoids/ total photosynthetic pigment
ylm-1	0.87b	0.66b	0.49b	1.54b	0.31a
ylm-2	0.88b	0.56b	0.46b	1.44b	0.32a
豫谷1号Yugu 1	1.21a	1.41a	0.83a	2.62a	0.32a

株系 Lines	气孔密度 Stomatal density /(N·mm^-2)	气孔长度 Stomatal length /μm	气孔宽度 Stomatal width /μm	气孔导度 Stomatal conductance /(mol·m^-2·s^-1)
ylm-1	512.7a	25.4a	18.4a	4.19a
ylm-2	503.2a	26.1a	19.7a	4.08a
豫谷1号 Yugu 1	499.8a	24.6a	17.9a	3.74b

Study on Photosynthetic Characteristics of Foxtail Millet Mutant With Yellow Leaf Colour

谷子黄叶色突变体光合特性研究

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Figures/Tables 8

References 32

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株系 Lines	PSⅡ最大荧光F_m	PSⅡ初始荧光F₀	PSⅡ原初光能转化效率F_v/F_m	非光化学淬灭系数qN	电子传递速率 ETR
ylm-1	1.85×10⁴a	3.78×10³b	0.79a	2.72b	306.7a
ylm-2	1.87×10⁴a	3.66×10³b	0.73a	2.80b	314.8a
豫谷1号 Yugu 1	1.77×10⁴b	4.44×10³a	0.68b	3.35a	265.3b

株系 Lines	Rubisco活性 Rubisco activity/(U·g^-1)	PEPCase活性 PEPCase activity/(IU·g^-1)	NADP-ME活性 NADP-ME activity/(mIU·g^-1)
ylm-1	0.468b	0.789a	1.75×10⁴a
ylm-2	0.485b	0.796a	1.79×10⁴a
豫谷1号 Yugu 1	0.711a	0.640b	1.55×10⁴b

株系 Lines	株高 Plant height /cm	穗长 Spike length /cm	穗粗 Spike diameter /mm	单穗质量 Per spike weight/g	穗粒质量 Grain weight per spike/g	千粒质量 Thousand grain weight/g
ylm-1	178.2a	23.6a	24.9a	17.6a	12.9a	2.64a
ylm-2	175.7a	22.8a	23.3a	18.5a	12.8a	2.62a
豫谷1号 Yugu 1	158.1b	18.5b	19.6b	9.6b	6.9b	2.58b

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