Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (8): 1794-1801.DOI: 10.11869/j.issn.100-8551.2021.08.1794

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles     Next Articles

Expression and Function of Cauliflower BobERF17, A Member of the AP2/ERF Transcription Factor Family, In Response To Abiotic Stresses

LI Hui1,*, YANG Yaling1,*, LI Cong2, LI Lihong2, HAN Zhanpin1, WANG Chunguo2   

  1. 1College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384;
    2College of Life Sciences, Nankai University, Tianjin 300071
  • Received:2020-04-30 Revised:2020-07-14 Online:2021-08-10 Published:2021-06-11

花椰菜AP2/ERF转录因子家族成员BobERF17响应逆境胁迫的表达及功能研究

李慧1,*, 杨亚苓1,*, 李聪2, 李丽红2, 韩占品1, 王春国2   

  1. 1天津农学院园艺园林学院,天津 300384;
    2南开大学生命科学学院,天津 300071
  • 通讯作者: *李慧,女,副教授,主要从事园艺植物生物技术研究。E-mail: lihui@tjau.edu.cn;杨亚苓,女,主要从事园艺植物生物技术研究。E-mail: 316639382@qq.com。同为通讯作者。
  • 作者简介:李慧,女,副教授,主要从事园艺植物生物技术研究。E-mail: lihui@tjau.edu.cn
  • 基金资助:
    国家自然科学基金(31872115),天津市科技计划种业科技重大专项(18ZXZYNC00160)

Abstract: AP2/ERF is a plant-specific transcription factor superfamily, among which the members of ERF family have been identified to play important roles in abiotic stress responses in plants. To further understand the role of ERFs in cauliflower, in the present study, BobERF17, a member of the ERF family in cauliflower, was cloned, and the transcriptional expression and function of BobERF17 were further explored. Sequence analysis showed that BobERF17 coding region is 576 bp in length and encodes a protein consisting 191 amino acids with unknown function. Cluster analysis showed that BobERF17 had a high similarity to the ERF17 sequences in dicotyledons, especially Brassica plants, but differed greatly from the ERF17 transcription factors in monocotyledons. Analysis of expression profiles under different stress conditions confirmed that BobERF17 displayed significantly up-regulated expression characteristics under drought and high temperature stresses, but was not sensitive to salt and low temperature stresses. Subsequently, the BobERF17 overexpression vector was successfully constructed and transformed into Arabidopsis. Overexpressed BobERF17 Arabidopsis homozygous lines were obtained. Functional analysis showed that the overexpression of BobERF17 in Arabidopsis did not affect the growth and development of the transgenic lines, but could significantly increase their tolerance to drought and high temperature stresses. The results confirmed that BobERF17 may play an important role in positive response to drought and high temperature stresses in cauliflower. These findings provided new insight into the function and regulation of BobERF17 in cauliflower.

Key words: cauliflower, BobERF17, abiotic stress, salt and drought tolerance

摘要: AP2/ERF为植物特有的一类转录因子超家族,其中ERF家族成员被证实在植物逆境胁迫响应中发挥重要作用。为研究花椰菜中不同ERF转录因子的功能,本研究对花椰菜ERF家族中的一个成员BobERF17进行了克隆、表达及功能探究。序列分析显示,BobERF17编码区全长576 bp,编码一个由191个氨基酸组成的蛋白。聚类分析表明,BobERF17与双子叶植物,特别是芸薹属植物中的ERF17序列具有较高相似性,但与单子叶植物中该转录因子序列差异很大。不同逆境胁迫处理条件下的表达谱分析证实,BobERF17在干旱及高温胁迫条件下均呈现显著上调表达,但对盐及低温胁迫响应不敏感。将构建的BobERF17过表达载体转化入拟南芥,获得过表达BobERF17拟南芥纯合株系。功能分析显示,在拟南芥中过表达BobERF17对转化株的生长发育无明显影响,但可以显著提高转化株对干旱及高温胁迫的耐受。本研究结果证实,BobERF17在花椰菜干旱及高温胁迫中可能发挥重要的正向调控作用,为进一步探究BobERF17的功能及调控机制奠定了基础。

关键词: 花椰菜, BobERF17, 逆境胁迫, 耐盐抗旱