Abstract: In order to clarify the effect of different cropping patterns on alleviating continuous cropping obstacles in broccoli continuous cropping fields, Illumina Miseq high-throughput sequencing technology was used in this study to analyze and compare the effects of three different treatments (paddy-upland rotation of rice and broccoli, drought rotation of pumpkin and broccoli, and continuous cropping of broccoli) on the bacterial community structure and diversity in the rhizosphere soil of broccoli in broccoli planting fields of coastal beach area in Taizhou Bay, Zhejiang Province. The results showed that 28 phyla, 92 classes, 167 orders, 301 families and 691 genera of soil bacteria were obtained from the rhizosphere soil of broccoli by the three cropping patterns in Taizhou estuary, Zhejiang province. Proteobacteria, Acidobacteria, Actinomycetes,Gemmatimonadetes, Bacteroidetes, Chloroflexi, Cyanobacteria, Firmicutes, Nitrospirae and Verrucomicrobia were the dominant phyla with high relative abundance of bacteria in broccoli rhizosphere soil in Taizhou estuary coastal beach. Paddy-upland rotation could improve the relative abundance of Proteobacteria, Acidobacteria and Chloroflexi, while decrease the relative abundance of Actinobacteria, Gemmatimonadetes, Bacteroidetes and Cyanobacteria in rhizosphere soil of broccoli. The diversity index and richness index were the highest in the paddy-upland rotation, followed by drought rotation samples, and the broccoli continuous cropping was the lowest. Therefore, compared with long-term continuous cropping of broccoli, both paddy-upland rotation and drought rotation could improve the bacterial community structure of broccoli rhizosphere soil in Taizhou estuary coastal beach. However, paddy-upland rotation had better effects on improving the abundance and the diversity index of beneficial bacteria in broccoli rhizosphere soil and improving the ecological environment of broccoli rhizosphere. The finding provides the theoretical and practical evidence for broccoli cultivation of high yield and excellent quality in this region.

Key words: high-through sequencing, bacterial community, paddy-upland rotation, broccoli, coastal reclaimation areas

摘要： 为明确不同种植方式减缓西兰花的连作障碍,本研究采用Illumina Miseq高通量测序技术,分析和比较了浙江省台州湾滨海滩涂地西兰花种植区单季稻-西兰花水旱轮作、南瓜-西兰花旱旱轮作和西兰花连作3种种植方式对西兰花根际土壤细菌群落结构和多样性的影响。结果表明,3种种植方式西兰花根际土壤共获得28门、92纲、167目、301科、691属土壤细菌。变形菌门、酸杆菌门、放线菌门、芽单胞菌门、拟杆菌门、绿湾菌门、蓝细菌门、厚壁菌门、硝化螺旋菌门、疣微菌门是台州湾滩涂围垦地西兰花根际土壤细菌相对丰度较高的优势菌群。水旱轮作能提高西兰花根际土壤细菌变形菌门、酸杆菌门和绿湾菌门的相对丰度,但降低了西兰花根际土壤细菌放线菌门、芽单胞菌门、拟杆菌门和蓝细菌门的相对丰度。多样性指数和丰富度指数均以水旱轮作为最高,其次是旱旱轮作,以西兰花连作的土壤多样性指数和丰富度指数最低。因此,与西兰花长期连作相比,水旱轮作和旱旱轮作均能改善台州湾滨海滩涂地区西兰花根际土壤细菌群落结构。水稻-西兰花水旱轮作更能增加西兰花根际土壤细菌有益菌丰度,提高其根际土壤细菌多样性指数,以改良西兰花根际生态环境。本研究结果为实现该区域西兰花优质高产栽培提供了理论和实践依据。

关键词: 高通量测序, 细菌群落, 水旱轮作, 西兰花, 滩涂地