Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (5): 1136-1146.DOI: 10.11869/j.issn.100-8551.2021.05.1136

• Food Irradiation·Food Science • Previous Articles     Next Articles

Effect of Liquid Nitrogen Freezing on Changes in Nutrients of Soft-Shell Crabs of Scylla paramamosain During Its Frozen Storage

GUO Liping, ZHANG Limin, MU Changkao, YE Yangfang*, WANG Chunlin   

  1. School of Marine Science, Ningbo University, Ningbo, Zhejiang 315211
  • Received:2020-02-07 Revised:2020-05-08 Online:2021-05-10 Published:2021-03-16

液氮冻结软壳青蟹在冻藏期间的营养物质变化

郭丽萍, 张丽敏, 母昌考, 叶央芳*, 王春琳   

  1. 宁波大学海洋学院,浙江 宁波 315211
  • 通讯作者: *叶央芳,女,研究员,主要从事代谢组学研究。E-mail:yeyangfang@nbu.edu.cn
  • 作者简介:郭丽萍,女,主要从事食品加工与安全研究。E-mail:2746034564@qq.com
  • 基金资助:
    国家自然科学基金项目(41776164),浙江省重大科技专项(2016C02055-8),现代农业产业技术体系专项(CARS-48),宁波大学王宽诚幸福基金资助

Abstract: To better understand the effect of liquid nitrogen (LN) freezing on changes in nutrients of soft-shell green mud crab (Scylla paramamosain) with during its frozen storage, a nuclear magnetic resonance (NMR)-based metabolomics technique was used to monitor metabolic profiles of edible tissues of soft-shell crabs with LN freezing during short-term (7 days) and long-term (8 months) frozen storage(-20℃). The results showed that short-term frozen storage and LN freezing showed no significant effect on crab metabolic profiles. However, the muscle dimethylamine level was tripled whereas the hepatopancreas inosine level was doubled after short-term frozen storage. In contrast, LN freezing could inhibit the increase of these two metabolites. Besides, long-term frozen storage with LN freezing lead to a significant increase in the muscle inosine, maltose, and glucose as well as hepatopancreas inosine, glutamine, and uridine of soft-shell crabs. These findings demonstrated the effectiveness of LN freezing to ameliorate the quality deterioration of soft-shell crab during its frozen storage. This study provide a theoretical reference for understanding the quality change of soft shell crobs during frozen storage.

Key words: mud crab (Scylla paramamosain), soft-shell crab, liquid nitrogen freezing, nuclear magnetic resonance, multivariate data analysis

摘要: 为探讨液氮(LN)冻结拟穴青蟹软壳蟹在冻藏期间的营养物质变化,本研究采用基于核磁共振(NMR)的代谢组学技术分析了LN冻结软壳蟹可食组织代谢谱在短期(7 d)和长期(8个月)冻藏期间的变化。结果表明,-20℃冻藏和LN冻结均对短期冻藏的软壳蟹代谢谱无显著影响(P>0.05),但 -20℃ 冻藏导致软壳蟹肌肉的二甲胺和肝胰腺的肌苷水平倍增,而LN冻结能抑制这两种物质的增加。长期冻藏可导致LN冻结软壳蟹肌肉的肌苷、麦芽糖和葡萄糖以及肝胰腺的肌苷、谷氨酰胺和尿苷水平显著升高(P<0.05)。表明LN冻结可有效缓解长期冻藏软壳蟹的质量下降。本研究结果为揭示软壳蟹冻藏过程中的品质变化提供了理论参考。

关键词: 拟穴青蟹, 软壳蟹, 液氮冻结, 核磁共振, 多变量数据分析