Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (5): 1030-1038.DOI: 10.11869/j.issn.100-8551.2021.05.1030

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles     Next Articles

Analysis of Flavonoids Content and Different Components in Leaves of Chinese Dwarf Cherry Germplasm

REN Yuqin1, FU Hongbo1,2, LEI Chen1, WANG Pengfei1, MU Xiaopeng1, ZHANG Jiancheng1, DU Junjie1,*   

  1. 1College of Horticulture, Shanxi Agriculture University, Taigu, Shanxi 030801;
    2Science and Technology for Rural Revitalization Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang 150000
  • Received:2020-08-13 Revised:2020-10-04 Online:2021-05-10 Published:2021-03-16

欧李种质叶片类黄酮含量及不同组分特征研究

任玉琴1, 付鸿博1,2, 雷晨1, 王鹏飞1, 穆霄鹏1, 张建成1, 杜俊杰1,*   

  1. 1山西农业大学园艺学院,山西 太谷 030801;
    2黑龙江省农业科学院乡村振兴科技研究所,黑龙江 哈尔滨 150000
  • 通讯作者: *杜俊杰,男,教授,主要从事欧李栽培与育种研究。E-mail:djj738@163.com
  • 作者简介:任玉琴,女,主要从事园艺植物种质创新与利用研究。E-mail:364503517@qq.com
  • 基金资助:
    山西省重点研发计划项目(201703D221028-4),山西省重点研发计划重点项目(201703D211001-04-04),山西省应用基础研究项目(201801D121251)

Abstract: To screen out the varieties with the highest flavonoid content and substance with antioxidant and tyrosinase activity in leaves of Chinese Dwarf Cherry. This study used mature leaves from 38 Chinese Dwarf Cherry germplasms as materials, the concentrations of catechin, epicatechin, liquiritigenin, rutin, quercetin, quercetin -7-O- glucoside, myricetin, glabridin and phloretin were determined by Ultra Performance Liquid Chromatography (UHPLC), and the antioxidant activity and the tyrosinase inhibitory ability were analyzed. The results showed that the flavonoid content of variety Y09-14 was 64.84 mg·g-1, the highest among 38 germplasms. Moreover, the Coefficient of variation of flavonoid and its 9 components among all varieties were over 20%, indicating there were rich genetic diversities. Cluster analysis based on flavonoid content in leaves showed that more than 70% of germplasms were classed as medium flavonoid level. By measuring the content of flavonoids and different components in leaves of different germplasms, it was found that catechin, epicatechin, rutin and quercetin -7-O- glucoside could be detected in all 38 germplasms, and catechin content was 6.271-935.295 mg·100g-1, which was significantly higher than the content of other eight substances. Catechin, epicatechin, rutin and quercetin -7-O- glucoside were positively correlated with DPPH scavenging rate, indicating that these four components had strong antioxidant capacity. Rutin, quercetin -7-O- glucoside and glabridin were positively correlated with tyrosinase inhibition rate. This study provides the basis for the detection and extraction of related substances in Chinese Dwarf Cherry in future studies.

Key words: Chinese Dwarf Cherry, leaf, flavonoids, component

摘要: 为筛选出欧李叶片类黄酮和9种类黄酮物质中单一物质含量较高的品种,并分析确定具有抗氧化和抑制酪氨酸酶活性的物质,本研究以38份欧李种质基生枝成熟期叶片为试材,利用超高效液相色谱法(UHPLC)测定儿茶素、表儿茶素、甘草素、芦丁、槲皮素、槲皮素-7-O-葡萄糖苷、杨梅素、光甘草定、根皮素活性物质含量,对其抗氧化及抑制酪氨酸酶能力进行分析。结果表明,38份欧李种质叶片中,Y09-14品种类黄酮含量最高,为64.84 mg·g-1,且类黄酮及其9种组分的变异系数均超过20%,表明其遗传多样性丰富。根据叶片类黄酮含量进行聚类分析,发现70%以上的种质为中类黄酮类型。通过对不同种质欧李叶片类黄酮及不同组分物质含量的测定发现,儿茶素、表儿茶素、芦丁、槲皮素-7-O-葡萄糖苷在38份种质中均能被检测到,且儿茶素含量为6.271~935.295 mg·100 g-1,极显著高于另外8种物质。4种不同活性物质(儿茶素、表儿茶素、芦丁、槲皮素-7-O-葡萄糖苷)与DPPH清除率呈极显著正相关(P<0.01),表明这种活性物质的抗氧化能力较强;芦丁、槲皮素-7-O-葡萄糖苷、光甘草定与酪氨酸酶抑制率呈正相关。本研究为欧李后期相关物质的检测及提取提供了依据。

关键词: 欧李, 叶片, 类黄酮, 组分