Abstract: In order to study the effects of radiation mutagenesis and the protective effects of three protective agents on Gardenia jasminoides cuttings, the cuttings was firstly irradiated by ⁶⁰Co-γ rays with different doses (5, 10, 20, 40 Gy), then soaked in three kinds of protective agents including sodium chloride (NaCl), cysteine (Cys) and vitamin C (Vc), with four different concentrations. After that, the indexes of rooting rate, survival rate, root length, number of roots, planting rate, internode length, number of branches, number of leaves, and leaf length, leaf width of gardenia cuttings had been analyzed. Damages of the cuttings gradually increased with the increase of radiation dose. The half-rooting dose of ⁶⁰Co-γ rays for gardenia cuttings was 21.22 Gy; half-lethal dose was 81.65 Gy, and half-planting dose was 20.30 Gy. Among the treatments, 3.00 g·L^-1 NaCl had good effects on rooting and growth of gardenia cuttings at 20 Gy, 0.12 g·L^-1 Cys and 0.08 g·L^-1 Vc had good effects at 40 Gy. All the three protective agents performed well on protecting the cuttings against damages under high dose ⁶⁰Co-γ rays, the protective effects were dependent on radiation dose and the concentration of protective agents. This research can provide a certain technical reference for the radiation mutation breeding of gardenia and the application of protective agents.

Key words: Gardenia jasminoides, radiation mutagenesis, protective agent, protective effect

摘要： 为研究⁶⁰Co-γ射线对栀子插条的辐射诱变作用及3种保护剂的保护效应,采用不同辐射剂量(5、10、20、40 Gy)辐射栀子插条,运用氯化钠(NaCl)、半胱氨酸(Cys)及维生素C(Vc)3种保护剂,分别设置4种不同浓度浸泡辐射后插条,分析不同辐射剂量及保护剂处理栀子插条的生根率、存活率、根长、根数、成株率、节间长度、分枝数、叶片数、叶长、叶宽等指标。结果表明,随着辐射剂量的增加,栀子插条的损伤逐渐增大。栀子插条⁶⁰Co-γ射线半生根剂量为21.22 Gy,半致死剂量为81.65 Gy,半成株剂量为20.30 Gy。3种保护剂在较高剂量辐射下具有显著的保护效应。在20 Gy辐射剂量下,3.00 g·L^-1 NaCl处理对栀子插条生根、生长保护效果较好。40 Gy辐射剂量下,0.12 g·L^-1 Cys和0.08 g·L^-1 Vc处理对栀子插条生根、生长保护效果较好。综上所述,3种保护剂均对较高剂量⁶⁰Co-γ射线损伤具有较好的保护作用,且保护效果与辐射剂量、保护剂浓度相关。本研究为栀子的辐射诱变育种及保护剂应用提供了技术参考。

关键词: 栀子, 辐射诱变, 保护剂, 保护效应