Transcriptome and Jasmin Metabolism Gene Analysis of Passiflora edulia Sims Under Low Temperature Stress

doi:10.11869/j.issn.100-8551.2021.04.0815

Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (4): 815-825.DOI: 10.11869/j.issn.100-8551.2021.04.0815

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles Next Articles

Transcriptome and Jasmin Metabolism Gene Analysis of Passiflora edulia Sims Under Low Temperature Stress

WEI Xiaoxia, WANG Xiao'an, CHEN Jin, LAI Ruilian, WU Rujian^*

Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013

Received:2019-12-18 Online:2021-04-10 Published:2021-02-06

百香果低温胁迫转录组及茉莉酸代谢基因分析

韦晓霞, 王小安, 陈瑾, 赖瑞联, 吴如健^*

福建省农业科学院果树研究所,福建福州 350013

通讯作者: ^*吴如健,男,研究员,主要从事果树选育种与栽培技术研究。E-mail:wurujian@126.com
作者简介:韦晓霞,女,副研究员,主要从事果树选育种与栽培技术研究。E-mail:wxx82046@qq.com
基金资助:
福建省科技厅项目(2019R11010030-7),福建省农业科学院科研项目(MNGY2018、MN2019-2)

Abstract

Abstract: To study the response mechanism of Passiflora edulia Sims (P. edulia) to low temperature stress, P. edulia plants treated under 0℃ and 25℃ were used as materials for transcriptome sequencing and digging out genes related jasmonic acid (JA) metabolism on Illumina HiSeq sequencing platform. A total of 45.30 Gb clean data were obtained, and 39 521 unigenes and 5 311 different expression genes (DEGs) were assembled. According to GO classification, these DEGs were classed into cell component, molecular function and biological process. Among them, metabolic pathways in biological process contained most DEGs, including sterol biosynthesis, flavonoid lipidization, tyrosine metabolism, L-phenylalanine biosynthesis, cork-fat biosynthesis, glucosinolide metabolism and long-chain aliphatic acyl coenzyme A metabolism. KEGG pathway enrichment analysis showed that ribosome pathway, starch and sucrose metabolism pathway, plant hormone signal transduction pathway, plant and pathogen interaction pathway were important metabolic pathways of P. edulia in response to low temperature stress. Based on qRT-PCR, AOC, AOS, JAR1, MYC2, PYL and JAZ, the key genes of JA metabolism pathway in P. edulia were all up-regulated under low temperature stress while COI1 was down-regulated. The validation results were similar to the trends of FPKM values obtained by sequencing, indicating that the sequencing results were relatively accurate. Our research indicated that the response mechanism of JA to low temperature stress in P. edulia was basically consistent with that in model plants, but the regulation of genes such as COI1 and MYC2 needed further functional verification. These results would provide scientific references for further research on cold resistant mechanism of P. edulia.

Key words: Passiflora edulia Sims, low temperature stress, transcriptome, jasmonic acid, qRT-PCR

摘要： 为研究百香果低温胁迫响应机制,以紫果百香果(Passiflora edulia Sims)为试验材料在0℃下低温胁迫处理,以常温处理为对照组(CK),采用Illumina HiSeq测序平台进行转录组测序,并对茉莉酸代谢相关基因进行挖掘。结果显示,共获得百香果转录组数据45.30 Gb,组装得到39 521条Unigene和5 311 个差异基因;GO分类显示注释的Unigene分为细胞组件、分子功能及生物过程三大类,其中差异基因数量最多为生物过程大类的代谢过程,包括甾醇生物合成、类黄酮糖脂化、酪氨酸代谢、L-苯丙氨酸生物合成、软木脂生物合成、芥子油苷代谢及长链脂肪-酰基辅酶A代谢等。KEGG途径富集分析结果显示,核糖体途径、淀粉与蔗糖代谢途径、植物激素信号转导途径及植物与病原体互作途径为百香果响应低温胁迫的重要代谢途径。实时定量PCR(qRT-PCR)分析表明,百香果低温胁迫后其茉莉酸代谢途径相关基因AOC、AOS、JAR1、MYC2、PYL和JAZ均上调表达,该结果与测序获得FPKM值变化趋势较为相似,说明测序结果较为准确。但低温胁迫后,COI1的表达水平呈下调趋势。研究发现,在百香果中茉莉酸对低温胁迫的响应机制大体上与模式植物一致,关于COI1和MYC2等基因的调控方式还有待进一步功能验证。本研究结果为进一步明确百香果抗寒机制提供了科学参考。

关键词: 百香果, 低温, 转录组, 茉莉酸, 实时荧光定量PCR

WEI Xiaoxia, WANG Xiao'an, CHEN Jin, LAI Ruilian, WU Rujian. Transcriptome and Jasmin Metabolism Gene Analysis of Passiflora edulia Sims Under Low Temperature Stress[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(4): 815-825.

韦晓霞, 王小安, 陈瑾, 赖瑞联, 吴如健. 百香果低温胁迫转录组及茉莉酸代谢基因分析[J]. 核农学报, 2021, 35(4): 815-825.

References

[1] 刘永碧, 郑德超. 攀西地区西番莲引种栽培技术[J]. 中国南方果树, 2004, 33(6): 45-47
[2] 周红玲, 郑云云, 郑家祯, 郑开斌. 百香果优良品种及配套栽培技术[J]. 中国南方果树, 2015, 44(2): 121-124
[3] 霍丹群, 蒋兰, 马璐璐, 侯长军, 杨平. 百香果功能研究及其开发进展[J]. 食品工业科技, 2012, 33(19): 354-358
[4] 中国科学院中国植物志委员会. 中国植物志[M]. 北京: 科学出版社, 1999
[5] Liu S A, Li A D, Chen C H, Cai G J, Zhang L M, Guo C Y, Xu M.De novo transcriptome sequencing in Passiflora edulis Sims to identify genes and signaling pathways involved in cold tolerance[J]. Forests, 2017, 8(11): 435
[6] 董万鹏, 罗充, 龙秀琴, 胡静, 李燕. 低温胁迫对西番莲抗寒生理指标的影响[J]. 植物生理学报, 2015, 51(5): 771-777
[7] 陈颖, 陈昕, 汪南阳, 胡菲, 曹福亮. 低温胁迫下西番莲叶片的生理反应及超微结构变化[J]. 西北植物学报, 2012, 32(3): 532-539
[8] 徐呈祥. 提高植物抗寒性的机理研究进展[J]. 生态学报, 2012, 32(24): 7966-7980
[9] 沈漫, 王明庥, 黄敏仁. 植物抗寒机理研究进展[J]. 植物学报, 1997, 14(2): 1-8
[10] 王小华, 庄南生. 脯氨酸与植物抗寒性的研究进展[J]. 中国农学通报, 2008, 24(11): 398-402
[11] Guo X Y, Liu D F, Chong K.Cold signaling in plants: Insights into mechanisms and regulation[J]. Journal of Integrative Plant Biology, 2018, 60(9): 7-18
[12] Zhu J K.Abiotic stress signaling and responses in plants[J]. Cell, 2016, 167(2): 313-324
[13] Ding Y L, Li H, Zhang X Y, Xie Q, Gong Z Z, Yang S H.OST1 kinase modulates freezing tolerance by enhancing ICE1 stability in Arabidopsis[J]. Developmental Cell, 2015, 32(3): 278-289
[14] Gong X X, Yan B Y, Hu J, Yang C P, Li Y J, Liu J P, Liao W B.Transcriptome profiling of rubber tree (Hevea brasiliensis) discovers candidate regulators of the cold stress response[J]. Genes and Genomics, 2018, 40(11): 1181-1197
[15] 王海波, 黄椿颖, 庞学群, 徐兰英, 张昭其. 茉莉酸甲酯诱导的采后香蕉果实耐冷性与活性氧信号的关系[J]. 中国农业科学, 2008, 41(4): 1165-1171
[16] 段小华, 邓泽元, 宾金华. 茉莉酸甲酯对水稻幼苗抗冷性的影响[J]. 植物生理学通讯, 2009, 45(9): 881-884
[17] 于涌鲲, 赵福宽, 万善霞, 郝玉兰, 孙清鹏. Ca²+和茉莉酸对菠菜CBF表达的影响[J]. 中国农学通报, 2010, 26(21): 59-62
[18] 左彬彬, 张彦萍, 刘海河, 陈倩云, 李田. 茉莉酸甲酯对厚皮甜瓜幼苗耐冷性的影响[J]. 河北农业大学学报, 2018, 41(5): 75-79
[19] 刘悦萍, 黄卫东. 茉莉酸、脱落酸与胁迫信号转导[C]//中国青年农业科学学术年报, 北京: 中国农学会, 2002: 260-263
[20] Wu Q, Leng P S, H Z H. Cloning and expression analysis of allene oxide synthase gene Ls AOS in lilium ‘Siberia’[J]. Agricultural Biotechnology, 2018, 7(2): 8-12
[21] Isayenkov S, Mrosk C, Stenzel Ⅰ, Strack D, Bettina H.Suppression of allene oxide cyclase in hairy roots of Medicago truncatula reduces jasmonate levels and the degree of mycorrhization with Glomus intraradices[J]. Plant Physiology, 2005, 139(3): 1401
[22] Kuroda H, Oshima T, Kaneda H, Takashio M.Identification and functional analyses of two cDNAs that encode fatty acid 9-/13-hydroperoxide lyase (CYP74C) in rice[J]. Journal of the Agricultural Chemical Society of Japan, 2005, 69(8): 1545-1554
[23] Von Malek B, Van Der Graaff E, Schneitz K, Keller B. The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway[J]. Planta, 2002, 216(1): 187-192
[24] Laudert D, Pfannschmidt U, Lottspeich F,Holländer-Czytko H, Weiler E.Cloning, molecular and functional characterization of Arabidopsis thaliana allene oxide synthase (CYP 74), the first enzyme of the octadecanoid pathway to jasmonates[J]. Plant Molecular Biology, 1996, 31(2): 323-335
[25] Riemann M, Riemann M, Takano M.Rice JASMONATE RESISTANT 1 is involved in phytochrome and jasmonate signalling[J]. Plant Cell and Environment, 2010, 31(6): 783-792
[26] Xie D X, Feys B F, James S, Nieto-Rostro M, Turner J G.COI1: An Arabidopsis gene required for jasmonate-regulated defense and fertility[J]. Science, 1998, 280(5366): 1091-1094
[27] Wang Y C, Xu H F, Liu W J, Wang N, Qu C Z, Jiang S H, Fang H C, Zhang Z Y, Chen X S.Methyl jasmonate enhances apple’ cold tolerance through the JAZ-MYC2 pathway[J]. Plant Cell, Tissue and Organ Culture, 2019, 136(1): 75-84
[28] Ba L J, Kuang J F, Chen J Y, Lu W J.MaJAZ1 attenuates the MaLBD5-mediated transcriptional activation of jasmonate biosynthesis gene MaAOC2 in regulating cold tolerance of banana fruit[J]. Journal of Agricultural and Food Chemistry, 2016, 64(4): 738
[29] Zhou X J, Yan S W, Sun C, Li S Z, Li J, Xu M Y, Liu X P, Zhang S J, Zhao Q Q, Li Y.A maize jasmonate Zim-domain protein, ZmJAZ14, associates with the JA, ABA, and GA signaling pathways in transgenic Arabidopsis[J]. PLoS One, 2015, 10(3): e121824
[30] Qi T C, Song S S, Ren Q C, Wu D W, Huang H, Chen Y, Fan M, Peng W, Ren C C, Xie D.The Jasmonate-ZIM-domain proteins interact with the WD-Repeat/bHLH/MYB complexes to regulate Jasmonate-mediated anthocyanin accumulation and trichome initiation in Arabidopsis thaliana[J]. Plant Cell, 2011, 23(5): 1795-1814
[31] Song S S, Qi T C, Huang H, Ren Q C, Wu D W, Chang C Q, Peng W, Liu Y, Peng J R, Xie D X.The jasmonate-ZIM domain proteins interact with the R2R3-MYB transcription factors MYB21 and MYB24 to affect jasmonate-regulated stamen development in Arabidopsis[J]. Plant Cell, 2011, 23(3): 1000-1013
[32] Chini A, Fonseca S, Fernández G, Adie B, Chico J M, Lorenzo O, García-Casado G, López-Vidriero Ⅰ, Lozano F M, Ponce M R, Micol J L, Solano R.The JAZ family of repressors is the missing link in jasmonate signalling[J]. Nature, 2007, 448(7154): 666-671
[33] Chini A, Boter M, Solano R.Plant oxylipins: COI1/JAZs/MYC2 as the core jasmonic acid-signalling module[J]. Febs Journal, 2010, 276(17): 4682-4692
[34] Aleman F, Yazaki J, Lee M, Takahashi Y, Kim A Y, Li Z X, Kinoshita T, Ecker J R, Schroeder J Ⅰ.An ABA-increased interaction of the PYL6 ABA receptor with MYC2 transcription factor: A putative link of ABA and JA signaling[J]. Scientific Reports, 2016, 6(1): 28941
[35] Mao X Z, Cai T, Olyarchuk J G, Wei L P.Automated genome annotation and pathway identification using the KEGG Orthology (KO) as a controlled vocabulary[J]. Bioinformatics, 2005, 21(19): 3787-3793
[36] Bateman A, Coin L, Finn R, Hollich Ⅴ, Griffiths-Jones S, Khanna A, Marshall M, Moxon S, Sonnhammer E, Studholme D, Yeats C, Eddy S.The Pfam protein families database[J]. Nucleic Acids Research, 2000, 28(1): 263-266
[37] Young M D, Wakefield M J, Smyth G K, Oshlack A.Gene ontology analysis for RNA-seq: accounting for selection bias[J]. Genome Biology, 2010, 11(2): R14
[38] Livak K J, Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCt method[J]. Methods, 2001, 25: 402-408
[39] Erlich Y, Mitra P, DelaBastide M, McCombie W R, Hannon G J. Alta-cyclic: A self-optimizing base caller for next-generation sequencing[J]. Nature Methods, 2008, 5(8): 679-682
[40] Risso D, Schwartz K, Sherlock G, Dudoit S.GC-content normalization for RNA-seq data[J]. BMC Bioinformatics, 2011, 12(1): 480
[41] 张喜春, 韩振海, Ходжайова ЛТ, Лутова ЛА. 植物体内甾醇的合成和生理作用[J]. 植物生理学报, 2001, 37(5): 452-457
[42] Gargiulo C E, Stuhlsatz-Krouper S M, Schaffer J E. Localization of adipocyte long-chain fatty acyl-CoA synthetase at the plasma membrane[J]. Journal of Lipid Research, 1999, 40(5): 881-892
[43] Yilmazer M, Stevens J F, Buhler D R.In vitro glucuronidation of xanthohumol, a flavonoid in hop and beer, by rat and human liver microsomes[J]. Febs Letters, 2001, 491(3): 256
[44] Seigler D S.Shikimic Acid Pathway[M]. US: Springer, 1998
[45] 陈亚州, 阎秀峰. 芥子油苷在植物-生物环境关系中的作用[J]. 生态学报, 2007(6): 2584-2593
[46] Vishwanath S J, Delude C, Domergue F, Rowland O.Suberin: Biosynthesis, regulation, and polymer assembly of a protective extracellular barrier[J]. Plant Cell Reports, 2015, 34(4): 573-586
[47] Gong J K, Liu J Z, Ronan E A, He F, Cai W, Fatima M, Zhang W Y, Lee H K, Li Z Y, Kim G H, Pipe K P, Duan B, Liu J F.A Cold-sensing receptor encoded by a glutamate receptor gene[J]. Cell, 2019, 178(6):1375-1386
[48] Sohn E Y, Kim Y H, Kim J T, Won J S, Lee Ⅰ J.Changes in endogenous abscisic acid, jasmonic acid and sucrose content during bulb development in the cold-type cultivar of garlic (Allium sativum L.) of Korea[J]. Korean Journal of Horticultural Science and Technology, 2011, 29(1): 1-9
[49] Ketchie D O.The effect of gibberellin applications and defoliation during summer on cold resistance of apple trees the following winter[J]. Acta Horticulturae (Netherlands), 1978, 1(81): 73-76
[50] 陈丹, 王卫安, 岳全奇, 赵琴. 植物生长素响应冷胁迫反应的研究进展[J]. 植物生理学报, 2016, 52(7): 989-997
[51] 王三根. 细胞分裂素在植物抗逆和延衰中的作用[J]. 植物学报, 2000, 17(2): 121-126
[52] Feng Y Y, Zhang Z X, Dong X J, Liu X, Yan Y R, Wang J L,Wang X H, Shi S P.Expression analysis of allene oxide synthase gene from Aquilaria sinensis[J]. Acta Pharmaceutica Sinica, 2017, 52(12): 1962-1969
[53] Bruno D, Xue G P, Sprague S J, Kirkegaard J A, Ross J J, Reid J B, Fitt G P, Sewelam N, Schenk P M, Manners J M, Kazan K.MYC2 differentially modulates diverse jasmonate-dependent functions in Arabidopsis[J]. Plant Cell, 2007, 19(7): 2225-2245
[54] Lenka S K, Muthusamy S K, Chinnusamy V, Bansal K C.Ectopic expression of rice PYL3 enhances cold and drought tolerance in Arabidopsis thaliana[J]. Molecular Biotechnology, 2018, 60(5): 350-361

Metrics

Comments

创刊：1987年
主管部门：农业农村部
主办单位：中国原子能农学会与中国农业科学院农产品加工研究所（前中国农业科学院原子能利用研究所）
ISSN：1000-8551

访问统计
总访问：
今日访问：
当前在线：

Transcriptome and Jasmin Metabolism Gene Analysis of Passiflora edulia Sims Under Low Temperature Stress

百香果低温胁迫转录组及茉莉酸代谢基因分析

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Qingzhu, CAI Youming, ZHANG Yongchun, XU Junxu, YANG Liuyan, SUN Yi. Development and Application of SSR Molecular Markers Based on Transcriptome Sequencing of Lycoris spp. [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(9): 2002-2015.
[2]	XU Tao, LU Zhengzhao, XIA Dongjian, WAN Jing, JIANG Shuhan, SONG Jianghua. Cloning and Expression Analysis of BoFBX117 From Brassica oleracea [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(5): 1060-1066.
[3]	DING Haomiao, DU Haofei, FU Ruijie, JIN Xudong, XIE Ce, CHEN Tongni, WANG Caisheng, QIAN Guoying. Analysis of Differentially Expresses Genes in Human Erythroleukemia Cells Induced by Sulfated Polysaccharide Fraction From Sargassum fusiforme [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(3): 595-604.
[4]	DU Wenli, CHEN Zhongshan, XU Duanxiang, XU Tongwei, GAO Shan, WEN Qingfang. Physiological Response and Differentially Expressed Genes Analysis of Transcriptome in Momordica charantia L. Leaf Under Cold Stress [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(2): 338-348.
[5]	LI Chenyan, LI Zujun, TIAN Xuefei, FANG Jiahai, SHI Qinghua, ZENG Yongjun, LI Huijie, WU Ziming. Effects of Low Temperature Stress at Flowering Stage on Physiological Characteristics of Double Cropping Late Rice [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(11): 2634-2644.
[6]	LI Xuyan, YANG Zhongyi, JI Wei, YANG Mingxia. Excavation and Comparative Analysis of MYB Family Based on Hawthorn Flowers With Different Colors [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(1): 49-59.
[7]	LI Jin, LEI Bin, ZHAI Menghua, WANG Li, ZHANG Jungao, ZHOU Xiaoyun, LIANG Jing. Study on the Response Mechanism of the AsA-GSH Cycle in Cotton Seedling Under Low Temperature Stress [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(1): 221-228.
[8]	LIN Rongyan, WU Jianshe, LIN Bing, YE Xiuxian, ZHONG Huaiqin, HUANG Minling. Cloning and Expression Analysis of Uroporphyrinogen Decarboxylase Gene From Cymbidium Hybrid [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(9): 1898-1905.
[9]	PAN Xia, XU Yongjian, NING Yan, SHEN Xiquan. Effects of Temperature Stress on Genes’ Transcription and Expression in Juvenile Hippocampus kuda Bleeker [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(7): 1421-1431.
[10]	XU Duanxiang, ZHAO Ruili, CHEN Zhongshan, DU Wenli, XU Tongwei, GAO Shan. Transcriptome Sequencing and Analysis of Functional Genes in Young Leaves of Bottle Gourd [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(6): 1163-1177.
[11]	FANG Zhizhen, JIANG Cuicui, ZHOU Danrong, PAN Shaolin, YE Xinfu. Identification and Analysis of Aux/IAA Family Genes of Sanyueli (Prunus salicina Lindl.) and the Red-fleshed Mutant [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(2): 247-255.
[12]	ZHANG Wujun, LIU Baocai, ZHAO Yunqing, HUANG Yingzhen, CHEN Jingying. Physiological Responses and Cold Resistance Analysis of Camellia nitidissima Chi Under Low-temperature Stress [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(2): 401-408.
[13]	LIAN Dongmei, LAI Zhengfeng, YAO Yunfa, LIN Bizhen, HONG Jianji. Transcriptome Analysis of Okra Resistance for Meloidogyne incognita [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(12): 2692-2700.
[14]	ZHANG Bin, LI Meng, JIANG Maoshuang, WANG Junjie, HOU Siyu, HAN Yuanhuai, LI Hongying. Relationship Between Anthocyanin Accumulation and Response to Low Temperature Stress in Foxtail Millet [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(11): 2607-2613.
[15]	YANG Xu, YANG Zhiling, TAN Mei, CHENG Xiaoyan. Characteristics Analysis of Houpoëa officinalis Transcription and Development of EST-SSR Markers [J]. Journal of Nuclear Agricultural Sciences, 2019, 33(7): 1318-1329.