Alleviation of Copper Stress and Grey Correlation Analysis of Physiological Indexes of Maize Seeding by Soaking With Sucrose

doi:10.11869/j.issn.100-8551.2021.03.0753

Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (3): 753-759.DOI: 10.11869/j.issn.100-8551.2021.03.0753

• Isotope Tracer Technique·Ecology and Environment·Physiology • Previous Articles

Alleviation of Copper Stress and Grey Correlation Analysis of Physiological Indexes of Maize Seeding by Soaking With Sucrose

ZHAO Qi^1,*, GUO Yunhong², YANG Yuzhen¹, CHEN Lipei¹, LUO Qing¹

¹School of Life Science, Zhengzhou Normal University, Zhengzhou, Henan 450052;
²Department of Architectural Engineering, Zhengzhou Railway Vocational & Technical College, Zhengzhou, Henan 450044

Received:2019-10-28 Online:2021-03-10 Published:2021-01-19

蔗糖浸种缓解玉米幼苗铜胁迫及其生理指标的灰色关联分析

赵奇^1,*, 郭运宏², 杨玉珍¹, 陈丽培¹, 罗青¹

¹郑州师范学院,生命科学学院,河南郑州 450052;
²郑州铁路职业技术学院,建筑工程系,河南郑州 450044

通讯作者: ^*同第一作者。
作者简介:赵奇,女,讲师,主要从事农业生理、生物技术等方面的教学与科研工作。E-mail:zhq_612@163.com
基金资助:
河南省教育厅高等学校重点科研项目(19A180033),河南省科技攻关项目(182102310655),教育部人文社科项目(19YJCZH263)

Abstract

Abstract: In order to investigate the alleviation of maize seeding by soaking sugar to copper stress, maize was taken as experimental materials to study the effects of 342.3 mg·L^-1 sucrose soaking treatments on the physiological and biochemical indexes of maize seedlings treated with 0, 100, 200, and 400 mg·L^-1 CuSO₄, then Grey Correlation Analysis of between the physiological and biochemical indexes and recovery index of maize under copper stress were carried out. Our results showed that, sugar soaking could significantly improve plant height, relative plant height,root length, relative root length, superoxide dismutase (SOD), peroxidase (POD), and hydrogen peroxide(CAT) of maize seedlings. The ranking of grey relational degree of physiological and biochemical indexes and recovery index of maize under copper stress was: relative root length(0.799)> relative plant height (0.775)> root length(0.767)> proline content(0.749)>POD(0.74)> soluble protein content(0.699)> soluble sugar content(0.673)>CAT(0.664)> chlorophyll content(0.647)>SOD(0.601)> plant height(0.579). Thus, sucrose immersion could effectively relieve copper stress of maize seedlings to some extent. In order to improve the selection efficiency on copper resistance of sucrose soaked maize seed, we should strengthen the selection of strong correlation indexes such as relative root length, relative plant height, root length, proline content, POD, soluble protein content, soluble sugar content, CAT and so on. These results provide references for maize resistance breeding.

Key words: sucrose soaking, maize, the stress of copper, grey correlation analysis

摘要： 为探究蔗糖浸种缓解玉米幼苗铜胁迫的能力,采用浸种法研究了342.3 mg·L^-1蔗糖浸种处理对0、100、200、400 mg·L^-1硫酸铜处理玉米幼苗生理生化指标的影响,并进行生理生化指标与玉米铜胁迫恢复指数的灰色关联分析。结果表明,蔗糖浸种可显著提高一定程度铜胁迫下玉米幼苗的株高、根长、相对根长、相对株高、过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性。生理生化指标与蔗糖处理对铜胁迫恢复指数的灰色关联度排序为:相对根长(0.799)>相对株高(0.775)>根长(0.767)>脯氨酸含量(0.749)>POD活性(0.740)>可溶性蛋白含量(0.699)>可溶性糖含量(0.673)>CAT活性(0.664)>叶绿素含量(0.647)>SOD活性(0.601)>株高(0.579)。综上可知,蔗糖浸种在一定程度上能有效缓解玉米幼苗的铜胁迫,抗性鉴定时选择相对根长、相对株高、根长、脯氨酸含量、POD活性、可溶性蛋白含量、可溶性糖含量、CAT活性等强关联指标可以提高选择效率,这为玉米抗性育种提供了参考。

关键词: 蔗糖浸种, 玉米, 铜胁迫, 灰色关联分析

ZHAO Qi, GUO Yunhong, YANG Yuzhen, CHEN Lipei, LUO Qing. Alleviation of Copper Stress and Grey Correlation Analysis of Physiological Indexes of Maize Seeding by Soaking With Sucrose[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(3): 753-759.

赵奇, 郭运宏, 杨玉珍, 陈丽培, 罗青. 蔗糖浸种缓解玉米幼苗铜胁迫及其生理指标的灰色关联分析[J]. 核农学报, 2021, 35(3): 753-759.

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创刊：1987年
主管部门：农业农村部
主办单位：中国原子能农学会与中国农业科学院农产品加工研究所（前中国农业科学院原子能利用研究所）
ISSN：1000-8551

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Alleviation of Copper Stress and Grey Correlation Analysis of Physiological Indexes of Maize Seeding by Soaking With Sucrose

蔗糖浸种缓解玉米幼苗铜胁迫及其生理指标的灰色关联分析

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