Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (3): 745-752.DOI: 10.11869/j.issn.100-8551.2021.03.0745

• Isotope Tracer Technique·Ecology and Environment·Physiology • Previous Articles     Next Articles

Effects of Bio-Organic Fertilizer Fermented From Residue of Ginkgo biloba L. Leaf on Seed Germination and Sprout Growth of Brassica chinensis L.

SHAO Jinhua1,2,3, QUAN Qinguo1,2,3,4,*, ZHANG Wenbing1, XIONG Jiaxuan1, WEN Tianfeng1, YANG Chuanchuan1   

  1. 1Department of Biochemistry, Hunan University of Science and Engineering, Yongzhou, Hunan 425199;
    2Hunan Provincial Engineering Research Center for Ginkgo biloba, Yongzhou, Hunan 425199;
    3Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, Hunan University of Science and Engineering, Yongzhou, Hunan 425199;
    4Hunan Engineering Research Center of Soil Borne Disease of Green Control Technology, Yongzhou, Hunan 425199
  • Received:2020-07-06 Online:2021-03-10 Published:2021-01-19

银杏叶渣制肥对小白菜种子萌发及幼苗生长的影响

邵金华1,2,3, 全沁果1,2,3,4,*, 张文兵1, 熊佳萱1, 文天凤1, 杨川川1   

  1. 1湖南科技学院化学与生物工程学院,湖南 永州 425199;
    2湖南省银杏工程技术研究中心,湖南 永州 425199;
    3湘南优势植物资源综合利用湖南省重点实验室,湖南 永州 425199;
    4土传病害绿色防控湖南省工程研究中心,湖南 永州 425199
  • 通讯作者: *全沁果,男,助教,主要从事生物化学方面的研究与开发。E-mail:qinguo_quan@163.com
  • 作者简介:邵金华,女,高级实验师,主要从事生物技术方面的教学和科研。E-mail:jinhua0212@163.com
  • 基金资助:
    2017年校级重点课题“多菌种兼性固态发酵银杏液渣制备生物肥研究”(17XKY003),湖南省自然科学基金(2018JJ3194)

Abstract: In order to improve the added value of Ginkgo biloba leaves and reduce environmental pollution, humic acid content was used as evaluation index, and the single-factor orthogonal test was used to optimize the process parameters of EM strain to ferment Ginkgo biloba residue to prepare bio-organic fertilizer, the relevant components in organic fertilizer were also determined. Meanwhile, the efficiency of organic fertilizer for Ginkgo biloba residue was verified.Results showed that the optimized solid state fermentation process of organic fertilizer were fermentation duration 6 days, mate rial-liquid ratio 71.43%,Ginkgo biloba addition amount 26.95%, and glucose addition amount 1.62%. Under this condition,the content of humic acid was 39.36%. The effective living bacteria, organic matter and heavy metal content was in accordance with NY884-2012 biological organic fertilizer standards, which increased the germination index of Brassica Chinensis seeds, and promoted the development of Brassica Chinensis seedlings’ root system and plant were confirmed by hydroponic and pot experiments.After futher analysis,Brassica Chinensis seedlings were promoted by activating seedlings’ rhizosphere dehydrogenase and nitrate reductase, and quality of Brassica Chinensis were improved by promoting the accumulation of proline, soluble sugar, total flavonoids, and titratable acids in the cabbage seedlings. Therefore, Ginkgo biloba bio-organic fertilizer possesses practical application value to applied and popularized in agricultural production.

Key words: residue of Ginkgo biloba leaf, bio-organic fertilizer, Brassica chinensis, seed germination, seedling growth

摘要: 为了提高银杏叶的附加值,减少环境污染,本研究以腐殖酸含量为评价指标,采用正交试验优化EM菌种发酵银杏叶渣制备生物有机肥的工艺参数,并测定有机肥中的相关成分,同时验证银杏叶渣有机肥的肥效。结果表明,有机肥最适固态发酵工艺为:发酵时间6 d、液料比去离子水添加比例为71.43%、银杏叶渣添加比例为26.95%、葡萄糖添加比例为1.62%,此条件下测定的腐殖酸含量为39.36%。有效活菌数、有机质和重金属含量指标符合NY 884-2012生物有机肥标准;该有机肥可提高小白菜种子的发芽指数,促进小白菜幼苗根系和植株的发育,其机理可能是通过激活幼苗根际脱氢酶和硝酸还原酶提高小白菜幼苗的发育水平,另外,该有机肥可促进小白菜幼苗体内脯氨酸、可溶性糖、总黄酮和可滴定酸的积累,提高小白菜品质。银杏叶渣生物有机肥具有实际应用价值,可在农业生产中加以利用及推广。

关键词: 银杏叶渣, 生物有机肥, 小白菜, 种子萌发, 幼苗生长