Abstract:

In order to create compact waxy corn germplasms, Zheng58 (a compact common maize inbred line) and Tong759 (a expanded waxy maize line) were used as donor and recurrent parent, respectively. The favorable alleles of major QTL (qLA5) for leaf angle were transferred by backcross with seletion for leaf angle combined marker-assisted selection and 29 plants for BC₂F₂ were created. Through the background selection of 48 markers and the comprehensive identification of 18 traits such as plant type, stress resistance, yield and quality under high density, 4 new waxy germplasms with compact plant type, high yield, strong stress resistance and good quality were screened out. The average genomic content of the recurrent parent in 4 new germplasms increased from 78.48% to 88.67%, which can be used as parent materials for compact waxy maize breeding. The results of this study provide theoretical references for the improvement of waxy maize germplasms by using common maize and the breeding of new compact and high quality fresh eating waxy maize varieties.

Key words: waxy maize, molecular marker, leaf angle, compact germplasm

摘要：

为创制紧凑型糯玉米种质,本研究以紧凑型普通玉米自交系郑58为供体亲本,以平展型糯玉米自交系通759为轮回亲本,通过标记辅助回交结合叶夹角表型选择的方法,转移玉米叶夹角主效QTL(qLA5)有利等位基因,创制了29份紧凑型BC₂F₂单株。48个标记背景选择及高密度下株型、抗逆性、产量、品质等18个性状综合鉴定,筛选出株型紧凑、产量高、抗逆性强、品质优良的糯玉米新种质4份,使轮回亲本平均基因组含量由78.48%提高到88.67%,可作为紧凑型糯玉米育种的亲本材料。本研究结果为利用普通玉米改良糯玉米种质,选育紧凑型优质鲜食糯玉米新品种提供了理论参考。

关键词: 糯玉米, 分子标记, 叶夹角, 紧凑种质