Research Progress in Separation, Purification and Antihyperglycemic Activity of Sweet Potato Leaf Polyphenols

doi:10.11869/j.issn.100-8551.2021.02.0424

Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (2): 424-437.DOI: 10.11869/j.issn.100-8551.2021.02.0424

• Food Irradiation·Food Science • Previous Articles Next Articles

Research Progress in Separation, Purification and Antihyperglycemic Activity of Sweet Potato Leaf Polyphenols

LUO Dan, MU Taihua^*, SUN Hongnan

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing,Ministry of Agriculture and Rural Affairs, Beijing 100193

Received:2019-07-23 Online:2021-02-10 Published:2020-12-14

甘薯茎叶多酚分离纯化及降血糖活性研究进展

罗丹, 木泰华^*, 孙红男

中国农业科学院农产品加工研究所/农业农村部农产品加工综合性重点实验室,北京 100193

通讯作者: ^*木泰华,男,研究员,主要从事食品化学与营养方面研究。E-mail:mutaihua@126.com
作者简介:罗丹,女,主要从事食品科学与工程方面研究。E-mail:luodan0620@126.com
基金资助:
国家自然科学基金(31701614)

Abstract

Abstract: Sweet potato leaves are the aboveground parts of sweet potato, which can be harvested several times a year, and their yield is equal to that of sweet potato roots. Sweet potato leaves are rich in polyphenols, including phenolic acids and flavonoids. These compounds have been reported to possess many healthy benefits, such as antioxidant, antihyperglycemic, antimicrobial, anti-inflammatory, anti-tumor hepatoprotective, immunomodulatory, etc. Recently, more and more researchers pay attention to the antihyperglycemic activity of sweet potato leaf polyphenols. This article summarized the research progress in extraction, separation and purification of polyphenols from sweet potato leaves, and the individual phenolic composition and antihyperglycemic activity of sweet potato leaf polyphenols. The aim of this article is to provide a theoretical reference for the development and successful application of sweet potato leaf polyphenols in food and healthcare industries, and further enhance sustainable development of sweet potato leaf processing industry in China.

Key words: sweet potato leaves, polyphenols, extraction and purification, composition, antihyperglycemic activity

摘要： 甘薯茎叶为甘薯地上蔓生部分,一年能多次采收,与甘薯块茎产量相当。甘薯茎叶中的多酚类物质含量丰富,主要为酚酸类和黄酮类物质,具有抗氧化、降血糖、抗菌消炎、抗肿瘤、保护肝脏和免疫调节等作用,且甘薯茎叶多酚类物质的降血糖作用已成为天然产物活性研究领域的热点之一。本文综述了甘薯茎叶多酚类物质的提取和分离纯化方法、酚酸类和黄酮类物质的组分构成及体外、体内降血糖活性作用,并展望了甘薯茎叶多酚类物质降血糖活性的研究方向,旨在为甘薯茎叶多酚类物质在食品和医疗保健领域的开发应用提供理论参考,提高甘薯茎叶多酚类物质的有效开发利用率,进一步促进我国甘薯茎叶加工业的可持续发展。

关键词: 甘薯茎叶, 多酚, 提取纯化, 组分构成, 降血糖活性

LUO Dan, MU Taihua, SUN Hongnan. Research Progress in Separation, Purification and Antihyperglycemic Activity of Sweet Potato Leaf Polyphenols[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(2): 424-437.

罗丹, 木泰华, 孙红男. 甘薯茎叶多酚分离纯化及降血糖活性研究进展[J]. 核农学报, 2021, 35(2): 424-437.

References

[1] FAO (Food and Agriculture Organization). Food and agricultural commodities production [DB/OL]. (2019-01-18)[2019-07-23]. http://www.fao.org/faostat/zh/#data/QC
[2] Li M, Jang G Y, Lee S H, Kim M Y, Hwang S G, Sin H M, Kim S H, Lee J, Jeong H S.Comparison of functional components in various sweet potato leaves and stalks[J]. Food Science and Biotechnology, 2017, 26(1): 97-103
[3] 王永徐. 甘薯茎叶主要抗氧化功能成分的提取及其品种间差异研究[D].杭州:浙江农林大学, 2019
[4] Wang S N, Nie S P, Zhu F.Chemical constituents and health effects of sweet potato[J]. Food Research International, 2016, 89(1): 90-116
[5] Sun H N, Mu T H, Xi L S, Zhang M, Chen J W.Sweet potato (Ipomoea batatas L.) leaves as nutritional and functional foods[J]. Food Chemistry, 2014, 156: 380-389
[6] 句荣辉, 段丽丽, 王辉, 李晓红, 林少华, 贾红亮.甘薯叶开发利用及品质评价方法研究[J].现代农业科技, 2018(22): 249-251
[7] Kazhila C C.Diabetes mellitus and nature’s pharmacy of putative antidiabetic plants[J]. Journal of Herbal Medicine, 2018, 15: 1-12
[8] Taira J, Uehara M, Tsuchida E, Ohmine W.Inhibition of the β-catenin/tcf signaling by caffeoylquinic acids in sweet potato leaf through down regulation of the tcf-4 transcription[J]. Journal of Agricultural and Food Chemistry, 2014, 62(1): 167-172
[9] 涂宗财, 傅志丰, 王辉, 张露, 温庆辉, 李金林, 段邓乐, 李如一. 红薯叶不同溶剂提取物抗氧化性及活性成分鉴定[J].食品科学, 2015, 36(17):1-6
[10] Liao W C, Lai Y C, Yuan M C, Hsu Y L, Chan C F.Antioxidative activity of water extract of sweet potato leaves in Taiwan[J]. Food Chemistry, 2011, 127(3): 1224-1228
[11] Fu Z F, Tu Z C, Zhang L, Wang H, Wen Q H, Huang T.Antioxidant activities and polyphenols of sweet potato (Ipomoea batatas L.) leaves extracted with solvents of various polarities[J]. Food Bioscience, 2016, 15: 11-18
[12] Assefa A D, Keum Y S.Effect of extraction solvent and various drying methods on polyphenol content and antioxidant activities of yuzu (Citrus junos Sieb ex Tanaka)[J]. Journal of Food Measurement and Characterization, 2016, 11(2): 1-10
[13] 张文婷, 孔秀林, 孙健, 徐飞, 朱红, 岳瑞雪, 张毅, 钮福祥. 响应面法优化甘薯叶片多酚提取工艺[J]. 核农学报, 2018, 32(12): 2397-2405
[14] Zhu Z Z, Li S Y, He J R, Thirumdas R, Montesano D, Barba F J.Enzyme-assisted extraction of polyphenol from edible lotus (Nelumbo nucifera) rhizome knot: Ultra-filtration performance and HPLC-MS² profile[J]. Food Research International, 2018, 111: 291-298
[15] 李佩艳, 王锋, 尹飞, 刘建学, 戚艳艳, 熊瑞, 裴亚锋. 响应面法优化酶法提取红薯叶总黄酮的工艺[J]. 食品工业科技, 2012, 33(1): 275-281
[16] 徐艳. 超声波乙醇浸提法提取甘薯茎叶中总黄酮的工艺研究[J]. 食品研究与开发, 2017, 38(5): 76-79
[17] Zhu Z Z, Guan Q Y, Koubaa M, Barba F J, Roohinejad S, Cravotto G, Yang X S, Li S Y, He J R.HPLC-DAD-ESI-MS² analytical profile of extracts obtained from purple sweet potato after green ultrasound-assisted extraction[J]. Food Chemistry, 2017, 215: 391-400
[18] 田燕楠, 钟耀广. 响应面法优化超声辅助提取甘薯叶多酚工艺[J]. 山东农业大学学报(自然科学版), 2018, 49(5): 167-172
[19] Song J F, Li D J, Liu C Q, Zhang Y.Optimized microwave-assisted extraction of total phenolics (TP) from Ipomoea batatas leaves and its antioxidant activity[J]. Innovative Food Science and Emerging Technologies, 2011, 12(3): 282-287
[20] Moreira M M, Barroso M F, Boeykens A, Withouck H, Morais S, Cristina D.Valorization of apple tree wood residues by polyphenols extraction: Comparison between conventional and microwave-assisted extraction[J]. Industrial Crops and Products, 2017, 104: 210-220
[21] 张露, 卢遇, 涂宗财, 谢星, 沙小梅, 王辉, 傅志丰. 动态高压微射流预处理对甘薯叶多酚提取物抗氧化性的影响机制初探[J]. 食品与发酵工业, 2017, 43(6): 169-174
[22] Huang X Q, Tu Z C, Xiao H, Li Z, Zhang Q T, Wang H, Hua Y M, Zhang L.Dynamic high pressure microfluidization-assisted extraction and antioxidant activities of sweet potato (Ipomoea batatas L.) leaves flavonoid[J]. Food and Bioproducts Processing, 2013, 91(1): 1-6
[23] Zhang L, Tu Z C, Wang H, Fu Z F, Wen Q H, Chang H X, Huang X Q.Comparison of different methods for extracting polyphenols from Ipomoea batatas leaves, and identification of antioxidant constituents by HPLC-QTOF-MS²[J]. Food Research International, 2015, 70: 101-109
[24] Xiang Z, Wu X L.Ultrasonic-microwave assisted extraction of total flavonoids from Scutellaria baicalensis using response surface methodology[J]. Pharmaceutical Chemistry Journal, 2017, 51(4): 318-323
[25] 刘汉文, 陈洪兴, 龚晓钰. 超声微波协同提取甘薯叶中黄酮类化合物的研究[J]. 食品工业科技, 2014, 35(16): 298-301
[26] Liu J, Mu T H, Sun H N, Marie L F.Optimization of ultrasonic-microwave synergistic extraction of flavonoids from sweet potato leaves by response surface methodology[J]. Journal of Food Processing and Preservation, 2019, 43(5): 1-10
[27] 王丽萍, 徐传远. 酶-超声波法提取红薯茎叶中绿原酸的工艺研究[J]. 湖北农业科学, 2012, 51(12): 2574-2575, 2585
[28] Phong W N, Show P L, Chow Y H, Ling C T.Recovery of biotechnological products using aqueous two-phase systems[J]. Journal of Bioscience and Bioengineering, 2018, 126(3): 273-281
[29] 王永徐, 李臣, 邱天越, 叶夏芳, 崔鹏, 曹如霞, 陆国权. 超声耦合双水相提取甘薯叶多酚的响应面优化[J]. 江苏师范大学学报(自然科学版), 2018(4): 44-48
[30] 蒋益花, 蒋新龙. 甘薯叶绿原酸的微波协同双水相提取及其抗氧化活性[J]. 中国粮油学报, 2018, 33(8): 94-100
[31] Escobedo F Y, Chavez F D, Salmeron I, Molina G C, Perez V S.Optimization of supercritical fluid extraction of polyphenols from oats (Avena sativa L.) and their antioxidant activities[J]. Journal of Cereal Science, 2018, 80: 198-204
[32] 陈瑛, 孙兴力, 王文渊. 红薯叶中黄酮类化合物的超临界提取研究[J]. 广州化工, 2011, 39(6): 82-84
[33] Usuki T, Onda S, Fujita Y, Rikukawa M.Use of [C₄mim]Cl for efficient extraction of caffeoylquinic acids from sweet potato leaves[J]. Scientific Reports, 2017, 7(1): 1-7
[34] Xi L S, Mu T H, Sun H N.Preparative purification of polyphenols from sweet potato (Ipomoea batatas L.) leaves by AB-8 macroporous resins[J]. Food Chemistry, 2015, 172: 166-174
[35] Zhong J L, Muhammad N, Gu C Y, Yan W D.A simple and efficient method for enrichment of cocoa polyphenols from cocoa bean husks with macroporous resins following a scale-up separation[J]. Journal of Food Engineering, 2018, 243: 82-88
[36] 昝立峰, 李丹花, 殷春燕, 叶嘉,王帅. 大孔吸附树脂分离纯化紫甘薯色素的动力学分析[J]. 江苏农业科学, 2018, 46(10): 207-210
[37] Kelly N P, Kelly A L, Omahony J A.Strategies for enrichment and purification of polyphenols from fruit-based materials[J]. Trends in Food Science and Technology, 2019, 83: 248-258
[38] 吕玲玉. 甘薯叶与虎杖化学成分的分离及抑菌剂的跟踪和筛选[D]. 兰州: 兰州理工大学, 2009
[39] Kurata R, Yahara S, Yamakawa O, Yoshimoto M.Simple high-yield purification of 3,4,5-tri-o-caffeoylquinic acid from sweet potato (Ipomoea batatas L.) leaf and its inhibitory effects on aaldose reductase[J]. Food Science and Technology Research, 2011, 25(4): 760-767
[40] 刘雪辉, 李觅路, 谭斌, 陈惠衡, 陆英. 紫甘薯茎叶中绿原酸及异绿原酸对α-葡萄糖苷酶的抑制作用[J]. 现代食品科技, 2014, 30(3): 103-107
[41] Lei F, Zhang H, Zhou J, Geng Y L, Wang X.Rapid screening and preparative isolation of antioxidants from Alpinia officinarum hance using HSCCC coupled with DPPH-HPLC assay and evaluation of their antioxidant activities[J]. Journal of Analytical Methods in Chemistry, 2018, 2018(1): 1-6
[42] Li J Y, Dong G P, Li M L, Liu Z H, Lu Y.Efficient counter-current chromatographic isolation and structural identification of phenolic compounds from sweet potato leaves[J]. Journal of Liquid Chromatography, 2012, 35(11): 1517-1527
[43] 朱亚珠. 甘薯叶中咖啡酰奎尼酸类物质的分离纯化和高效液相色谱法分析[J]. 食品工业科技, 2015, 36(10): 73-82
[44] Lua X F, Zhou Y, Ren Y P, Zhang J.Improved sample treatment for the determination of flavonoids and polyphenols in sweet potato leaves by ultra performance convergence chromatography-tandem mass spectrometry[J]. Journal of Pharmaceutical and Biomedical Analysis, 2019, 169: 245-253
[45] Zheng W, Clifford M N.Profiling the chlorogenic acids of sweet potato (Ipomoea batatas) from China[J]. Food Chemistry, 2008, 106(1): 147-152
[46] Carvalho I S, Cavaco T, Brodelius M.Phenolic composition and antioxidant capacity of six artemisia species[J]. Industrial Crops and Products, 2011, 33(2): 382-388
[47] Zengin G, Locatelli M, Stefanucci A, Macedonio G, Adriano M. Chemical characterization, antioxidant properties, anti-inflammatory activity and enzyme inhibition of Ipomoea batatas L. leaf extracts[J]. International Journal of Food Properties, 2017(1): 1907-1919
[48] Luo C Y, Wang X X, Gao G, Wang L, Li Y X, Sun C J.Identification and quantification of free, conjugate and total phenolic compounds in leaves of 20 sweetpotato cultivars by HPLC-DAD and HPLC-ESI-MS/MS[J]. Food Chemistry, 2013, 141(3): 2697-2706
[49] Su X Y, Griffin J, Xu J W, Ouyang P, Zhao Z H, Wang W Q.Identification and quantification of anthocyanins in purple-fleshed sweet potato leaves[J]. Heliyon, 2019, 5: 1-6
[50] Tanaka M, Ishiguro K, Oki T, Okuno S.Functional components in sweet potato and their genetic improvement[J]. Breeding Science, 2019, 67(1): 52-61
[51] 罗建光, 孔令义. 巴西甘薯叶黄酮类成分的研究[J]. 中国中药杂志, 2005, 30(7): 516-518
[52] Lako J, Trenerry Ⅴ C, Wahlovist M, Wattanapenpaiboon N, Sotheeswaran S, Premier R.Phytochemical flavonols, carotenoids and the antioxidant properties of a wide selection of Fijian fruit, vegetables and other readily available foods[J]. Food Chemistry, 2007, 101(4): 1727-1741
[53] 江玉洁, 李美凤, 陈艳. 红薯叶中黄酮类化合物的研究进展[J]. 轻工科技, 2018(1): 8-10
[54] Khanam U K S, Oba S, Yanase E, Murakami Y. Phenolic acids, flavonoids and total antioxidant capacity of selected leafy vegetables[J]. Journal of Functional Foods, 2012, 4(4): 979-987
[55] Chu Y W, Chang L C, Hsu H F.Flavonoid content of several vegetables and their antioxidant activity[J]. Journal of the Science of Food and Agriculture, 2000, 80(5): 561-566
[56] 刘冉, 程霜, 王雷, 曾庆华, 张书贤. 不同品种甘薯叶提取物抗氧化及对α-葡萄糖苷酶抑制活性的研究[J].食品工业科技, 2019, 40(23):283-289
[57] Peng X, Zhang G W, Liao Y J, Gong D M.Inhibitory kinetics and mechanism of kaempferol on α-glucosidase[J]. Food Chemistry, 2016, 190: 207-215
[58] Zhang L, Tu Z C, Yuan T, Wang H, Xie X, Fu Z F.Antioxidants and α-glucosidase inhibitors from Ipomoea batatas leaves identified by bioassay-guided approach and structure-activity relationships[J]. Food Chemistry, 2016, 208(10): 61-67
[59] Zeng L, Zhang G W, Lin S Y, Lin S, Gong D.Inhibitory mechanism of apigenin on α-glucosidase and synergy analysis of flavonoids[J]. Journal of Agricultural and Food Chemistry, 2016, 64: 6939-6949
[60] 许乐, 逄晓阳, 芦晶, 张书文, 张瑞华, 刘鹭, 吕加平. HepG2细胞胰岛素抵抗模型的建立及其应用[J].核农学报, 2017, 31(9): 1775-1781
[61] Eid H M, Nachar A, Thong F, Sweeney G, Haddad P S.The molecular basis of the antidiabetic action of quercetin in cultured skeletal muscle cells and hepatocytes[J]. Pharmacognosy Magazine, 2015, 11(41): 74-81
[62] Chen L, Teng H, Cao T.Chlorogenic acid and caffeic acid from Sonchus oleraceus Linn synergistically attenuate insulin resistance and modulate glucose uptake in HepG2 cells[J]. Food and Chemical Toxicology, 2019, 127: 182-187
[63] Lee S L, Lee H K, Chin T Y, Tu S C, Kuo H M, Kao C M, Wu C Y.Inhibitory effects of purple sweet potato leaf extract on the proliferation and lipogenesis of the 3T3-L1 preadipocytes[J]. The American Journal of Chinese Medicine, 2015, 43(5): 1-11
[64] Lee S L, Chin T Y, Tu S C, Wang Y J, Hsu T Y, Kao M C, Wu Y C.Purple sweet potato leaf extract induces apoptosis and reduces inflammatory adipokine expression in 3T3-L1 differentiated adipocytes[J]. Evidence-based Complementary and Alternative Medicine, 2015, 2015: 1-9
[65] Lee L C, Lee S L, Chen C J, Chen H C, Kao M C, Liu C H, Chen J Y, Lai Y T, Wu Y C.Characterization of secondary metabolites from purple Ipomoea batatas leaves and their effects on glucose uptake[J]. Molecules, 2016, 21(6): 745-759
[66] Toong L J, Yi C C, Chia C L, Ting C L, Su Y L, Tzu C L, Jih M S.Sweet potato leaf extract inhibits the simulated in vitro gastrointestinal digestion of native starch[J]. Journal of Food and Drug Analysis, 2015, 23(3): 399-406
[67] Lin K H, Low P Y, Chao P Y, Shih M C, Chiang M C, Lai Y C, Wu S B.Antioxidant properties and glucose uptake effect of ethanol extracts from different sweet potato leaves prepared by lyophilization and oven-drying at 40℃[J]. Current Nutrition and Food Science, 2017, 13(999): 227-236
[68] 简磊, 符策岗, 揭勇. 2型糖尿病小鼠模型构建的研究进展[J]. 生命科学研究, 2019, 23(3): 237-244
[69] 祁秀茹, 王红杰. 高脂饮食联合链脲佐菌素诱导2型糖尿病大鼠模型的研究进展[J]. 河北医药, 2015, 37(21): 3308-3310
[70] Nagamine R, Ueno S, Tsubata M, Yamaguchi K, Takagaki K, Hira T, Hara H, Tsuda T.Dietary sweet potato (Ipomoea batatas L.) leaf extract attenuates hyperglycaemia by enhancing the secretion of glucagon-like peptide-1 (GLP-1)[J]. Food and Function, 2014, 5(9): 2309-2316
[71] Chang W C, Kuo P L, Chen C W, Wu J S, Shen S C.Caffeic acid improves memory impairment and brain glucose metabolism via ameliorating cerebral insulin and leptin signaling pathways in high-fat diet-induced hyperinsulinemic rats[J]. Food Research International, 2015, 77: 24-33
[72] Ogunrinola O O, Fajana O O, Olaitan S N, Adu O B, Akinola M O.Anti-diabetic activity of Ipomoea batatas leaves extract: Effects on hepatic enzymes in alloxan-induced diabetic rats[J]. Research Journal of Medicinal Plant, 2015, 9(5): 227-233
[73] Vivek K P, Alpana M, Mohammad F K, Poonam K.Activation of PERK-eIF2α-ATF4 pathway contributes to diabetic hepatotoxicity: Attenuation of ER stress by morin[J]. Cellular Signalling, 2019, 59: 41-52

Metrics

Comments

创刊：1987年
主管部门：农业农村部
主办单位：中国原子能农学会与中国农业科学院农产品加工研究所（前中国农业科学院原子能利用研究所）
ISSN：1000-8551

访问统计
总访问：
今日访问：
当前在线：

Research Progress in Separation, Purification and Antihyperglycemic Activity of Sweet Potato Leaf Polyphenols

甘薯茎叶多酚分离纯化及降血糖活性研究进展

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Yangping, WANG Jianhui, LIU Dongmin, LIU Yongle, HUANG Yiqun, WANG Faxiang, LI Xianghong, YU Jian. Optimization of Polyphenols Extraction From Lotus Seed Peel Waste and Its in Vitro Antioxidant Activities [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(6): 1376-1384.
[2]	HE Dan, JIANG Hongrui, YE Yafeng, YANG Yang, REN Yan, TAO Liangzhi, WU Yuejin, LIU Binmei. Analysis of the Mechanism of Early Senescence Degeneration in Rice Mutant ad1 [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(4): 769-779.
[3]	QIU Junkai, SUI Weice, MU Taihua, SUN Hongnan, CAO Qinghe. Comparative Study on the Nutritional and Functional Components of Sweet Potato Leaves From Fifty-Eight Cultivars [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(4): 911-922.
[4]	CAO Xi, YANG Dawei. Identification of Polyphenolic Compounds in Daylily and Influence of Blanching and Storage on Their Stability [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(12): 2787-2798.
[5]	XIE Tian, SUN Lin, FAN Wei, GUO Shiyin, XIAO Hang, TANG Zhonghai. Effect of Bound Polyphenols in Psidium Guajava on Mice Intestinal Flora Structure and Diversity [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(11): 2589-2597.
[6]	WANG Jin, WEI Saijin, WEI Limei. Effect of Straw Decomposition Agent on Rice Seedling Quality and the Substrate Microorganism [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(10): 2413-2422.
[7]	XU Yaozhao, SUN Wancang, FANG Yan, SUN Bolin. Changes of Endogenous Hormones and Total Polyphenols for Winter Turnip Rape (Brassica rapa L.) Subjected to Low Temperature [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(7): 1551-1560.
[8]	WANG Na, LI Ping, ZONG Yuzheng, ZHANG Dongsheng, HAO Xingyu. Study on the Decomposition Mechanism of Wheat Straw on Rain-fed Croplands in Northern China Under Different Patterns of Straw Returning Practice [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(7): 1613-1619.
[9]	WANG Xinyu, QIAN Jiamin, ZHANG Jinjie, YANG Wenge, LOU Qiaoming, XU Dalun. Effects of Cooking Methods on Nutritional Value of Wild and Cultured Freshwater Shrimp [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(6): 1257-1265.
[10]	CHEN Shengjun, YU Jiao, HU Xiao, YANG Xianqing, LI Laihao, QI Bo. Nutritional Analysis and Evaluation of Porphyra haitanensis in Shantou Area at Different Harvesting Stages [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(3): 539-546.
[11]	LU Junyu, ZHU Rui, WU Hejun, LEI Yanlin, XIAO Di, JIAO Chun, WEI Xueying, LIU Denghong. Effect of Tea Polyphenols on the Properties of Edible Potato Starch/Sodium Alginate Composite Films [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(10): 2226-2234.
[12]	YAO Yuehua, WANG Yaqin, JIA Xin, TANG Ning, CHENG Yongqiang. Effects of Tea Polyphenols Addition on the Quality and Antioxidant Properties of Noodles [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(10): 2261-2270.
[13]	MIAN Youming, LI Rong, HOU Xianqing, LI Peifu, WANG Xi'na. Effects of Straw Returning Combined With Decomposition Agents on Sandy Soil Properties and Growth of Maize Under Drip Irrigation [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(10): 2343-2351.
[14]	JIANG Mingjin, WANG Haiyue, HE Yan, WANG Chunyu, LI Na, YANG Zhiyuan, SUN Yongjian, MA Jun. Effects of Nitrogen Management on Lodging Resistance of Direct-seeded Rice [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(1): 157-168.
[15]	XU Caihong, JIN Weiquan, JIANG Zhongli, MIN Zhongman. Optimization of Extraction Technique of Polyphenols From Maize Bran and Its Antioxidant Activity [J]. Journal of Nuclear Agricultural Sciences, 2019, 33(9): 1774-1782.