20 June 1998, Volume 12 Issue 03
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J4. 1998, 12(03): 129-0. https://doi.org/10.11869/hnxb.1998.03.0129
Dry seeds of rice were irradiated by 4～8 MeV protons with various doses.The biological effect in M 1 was studied.The results show that protons were able to induce variation on chromosome structure in root tip cells.The rate of cells with chromosome aberration was increased with protons energy and dose added.The energy effects of protons were higher than dose effect; energy effects were showed between 8 MeV and 4,6 MeV.As compared with γ rays,the effects of protons were the same on chromosomal aberration and mitotic inhibition but higher on rate of cell with micronucleus and lower on rate of chromosomal bridge.The radiosensitivities of three rice varieties were various with different radio soures and references.
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J4. 1998, 12(03): 135-0. https://doi.org/10.11869/hnxb.1998.03.0135
Fresh keeping technology of ginseng by irradiation was studied and the results are as follows.The sutable dose is 0 4～0 6 kGy,and with this dose range,the ginseng is kept fresh for 12 months and the fresh keeping rate is over 97% .Comparing with the fresh ginseng,no notable difference in root hardness,colour and composition was found with irradiated ones.
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J4. 1998, 12(03): 139-0. https://doi.org/10.11869/hnxb.1998.03.0139
The mutagenic effect of Pingyangmycin on wheat was studied in this experiment and average root lengths of wheat plant treated by Pingyangmycin were 20 06cm,17 84cm and 17 07cm,respectively.lnhibiting effects on root length were stronger when treated by three concentration of Pingyangmycin than that with EMS treatment.Average root number of wheat treated by Pingyangmicin of 10μg/ml,30μg/ml and 50μg/ml were 4 59,3 97 and 3 53 respectively,obviously fewer than CK and 0 3% EMS treatment.M 1 seedling heights,were 7 39cm,6 93cm and 6 69cm when treated by Pingyangmycin of 10μg/ml,30μg/ml,50μg/ml,respctively,obviously lower than CK.Seedling emerging rates were 70%,69%,66%, when treated by Pingyangmycin of 10μg/ml, 30μg/ml,50μg/ml,respectively.Chromosomal aberration rates were 6 15%,7 36% and 5 18%, respectively.Mutation frequencies of M 2 were 2 25%,2 55% and 3 83%, respectively.The results indicate that Pingyangmycin has strong mutagenic effect and can be used as a new mutagenic agent.
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J4. 1998, 12(03): 146-0. https://doi.org/10.11869/hnxb.1998.03.0146
By mechanical grinding and sedimentating, the single cells and the fragments(less than 5 cells)of Spirulina platensis strains Sp Z were obtained.Then they were irradiaded with 60 Co gamma rays with their LD 50 of 1 2 kGy.After the single cells and the fragments were irradiaded with 2 4 kGy γ ray,a mutant [named Sp Z(E.L)] with extra long filaments was selected.The length of filaments,number of helix and length of helix of Sp Z(E.L) were 15,10 and 1 8 times of those of Sp Z,respectively.The growth curve of Sp Z was almost linear, while the growth curve of Sp Z(E.L) was S shaped.Moreover, the yield of Sp Z(E.L) was about 1 12 times of that of Sp Z cultured outdoors.
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J4. 1998, 12(03): 151-0. https://doi.org/10.11869/hnxb.1998.03.0151
An experiment was conducted to evaluate excretive tendency of zinc in feces and urine with 8 goats groups fed with diet Ⅰ (basal diet+ 65 Zn Met) and diet Ⅱ (basal diet+ 65 ZnSO 4) respectively. The results showed that 65 Zn concentration in feces of the goats fed with diet Ⅰ was lower than that of the goats fed with diet Ⅱ, and the absorptivity of zinc from Zn Met was significantly higher than that from 65 ZnSO 4(P<0 05).
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J4. 1998, 12(03): 157-0. https://doi.org/10.11869/hnxb.1998.03.0157
The dispersal ability of F 1 male Asian corn borer, Ostrinia furnacalis (Guenee), irradiated with 100, 150 and 200 Gy separately in parental generation were tested by marking(with calco oil red or Sudan blue internally) releasing recapturing (with synthesized sex pheromone) method in the field where the farthest distance from release point to pheromone trap was 550 m. The results showed that, as compared with the normal male moths, despite of the fact that a part of the irradiated F 1 males had lost dispersal ability or taxis to sex pheromone, there was no significant difference between the captured rates of irradiated F 1 males and normal males in the trap 550 m from release point, indicated that the dispersal ability or taxis to sex pheromone of irradiated F 1 males arrived at 550 m from release point are still well matched with the normal ones.
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J4. 1998, 12(03): 161-0. https://doi.org/10.11869/hnxb.1998.03.0161
Nutrient culture study was initiated to examine the synergistic mechanism of combinative application of bensulfuron and urea for weed control. The absorption of 14 C bensulfuron and their distribution in Lindernia procumbens (Krock.) Philcox were also investigated to determine the variation between two methods (combinative use of 14 C bensulfuron plus urea and 14 C bensulfuron alone). One hour after combinative application of 14 C bensulfuron plus urea, the highest amount of 14 C activity in L. procumbens were obtained. However, when 14 C bensulfuron applied alone, total absorption of 14 C activity was much lower in the 1st hour and then it slowly increased with time. The distribution of 14 C bensulfuron in root of L.procumbens plant was the highest and that in leaves was the lowest.
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J4. 1998, 12(03): 165-0. https://doi.org/10.11869/hnxb.1998.03.0165
Effects of the application of phosphorus and potassium fertilizer and deep rooting on reducing the absorption of 137 Cs by rice (especially in the seed) were estimated using pot and plot experiment. The results show that the available 137 Cs in soil decreased significantly by applying potassium fertilizer, which led to the lower accumulation of 137 Cs in rice stem and the most effective measure was to apply potassium sulphate of 922 5kg/ha . An unsteady effect with phosphate fertilizer existed. When P application was in a lower amount, the accumulation of 137 Cs in rice decreased. But following the increase of P application, the absorption of 137 Cs was promoted. The 137 Cs accumulation in rice decreased significantly by deep rooting.
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J4. 1998, 12(03): 171-0. https://doi.org/10.11869/hnxb.1998.03.0171
The leaching and vertical moving of 125 I in subtropics soils of south China were studied by soil volume. The results showed that after passing through 20cm soil volume, the content of 125 I in leachate of laterite was 5.26% of labelled amount and 11.05% in leachate of paddy soil. After leaching 88.03% of 125 I in laterite volume remained in the layer of 0～10 cm, while 92 21% in paddy soil volume. 27 days after vertical moving in soil volume, 125 I distributed in every layer, most of it remained in the topsoil, 91.67% of 125 I in laterite and 83.98% in paddy soil remained in the top layer (0～2cm).
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J4. 1998, 12(03): 175-0. https://doi.org/10.11869/hnxb.1998.03.0175
C 4 pyrimidinyl) bensulfuron methyl was synthesized from 14 C sodium cyanide with 6 steps. First, malonic acid ( 14 C carboxyl) was prepared, then changed to ethyl ( 14 C carboxyl) malonate by diazoethane. It reacted with guanidine nitrate, forming 14 C 2 amino 4,6 dihydroxy pyrimidine, which changed into 14 C 2 amino 4,6 dimethyloxy pyrimidine. This product reacted with methyl 2 (methylene sulfonyl isocyanate) benzoate, forming ( 14 C 4 pyrimidinyl) bensulfuron methyl. The radiochemical yield was 14 6%, radiochemical purity was more than 95%, and the specific activity was 28 9 kBq/mg.
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J4. 1998, 12(03): 179-0. https://doi.org/10.11869/hnxb.1998.03.0179
Milk samples were collected on 0, 10～12 and 22～24 days after service from 1224 dairy cows in 5 dairy farms. Progesterone concentration were examined by RIA. The reproductive status of the cows could be identified: pregnant(52 0%), nonfertilisation(22 7%), inactive ovary (5 8%), persistent CL, luteal cyst (3 8%),AI at inappropriate time (during luteal phase)(7 1%),abnormal oestrus cycles(8 6%),AI in pregnant animals etc. The examination results and our judgement were sent back to AI technician and veterinary of dairy farms as early as possible so that they could use these information (comparing with the rectal palpation results) to get correct diagnoses and to adopt appropriate remedial measures. So far 1801 oestrus cycles has been monitored.
