Journal of Nuclear Agricultural Sciences ›› 2020, Vol. 34 ›› Issue (8): 1855-1861.DOI: 10.11869/j.issn.100-8551.2020.08.1855

• Isotope Tracer Technique·Ecology & Environment·Physiology • Previous Articles     Next Articles

Effects of Phenanthrene on the Physiological and Biochemical Characteristics of Salix viminalis

LI Xia1,2, MA Xiaodong1, CHEN Yunhe3, ZHAI Feifei4, LIU Junxiang1, SUN Zhenyuan1, HAN Lei1,*   

  1. 1 Research Institute of Forestry/Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Chinese Academy of Forestry, Beijing 100091;
    2 College of Agriculture and Bioengineering(Peony Institute), Heze University, Heze, Shandong 274000;
    3 Beijing Academy of Forestry and Pomology Sciences, Beijing 100093;
    4 School of Architectural and Artistic Design, Jiaozuo, Henan 454000
  • Received:2019-10-09 Revised:2020-01-03 Online:2020-08-10 Published:2020-06-16

菲对蒿柳生理生化特性的影响

李霞1,2, 马晓东1, 程运河3, 翟飞飞4, 刘俊祥1, 孙振元1, 韩蕾1,*   

  1. 1 中国林业科学研究院林业研究所/国家林业局林木培育重点实验室,北京 100091;
    2 菏泽学院农业与生物工程学院(牡丹学院),山东 菏泽 274000;
    3 北京市林业果树科学研究院,北京 100093;
    4 河南理工大学建筑与艺术设计学院,河南 焦作 454000
  • 通讯作者: *韩蕾,女,研究员,主要从事观赏植物发育生理与分子生物学研究。E-mail:hdd@caf.ac.cn
  • 作者简介:李霞,女,讲师,主要从事观赏植物抗逆生理与分子生物学研究。E-mail:393362284@qq.com
  • 基金资助:
    中央级公益性科研院所基本科研业务费专项资金(CAFYBB2018ZB002),国家自然科学基金项目(31700533)

Abstract: To study theeffects of phenanthrene on physiology and biochemistry of Salix viminalis, a hydroponic experiment was conducted with cutting seedlings under different concentrations of phenanthrene(0.2, 0.5, 1.0 and 1.2 mg·mL-1), and 1.0 mg·L-1 phenanthrene was treated for 16days to measure the changes of photosynthetic characteristics and osmotic regulatory substances. The results showed that high concentration of phenanthrene significantly inhibited the plant height and root vigor of Salix viminalis, and increased conductivity and membrane permeability.Under the stress of 1.0 mg·L-1 phenanthrene, photosynthesis was inhibited with photochemical quenching (qP) decreased significantly, and the light reaction was influenced;stomatal conductance (Gs), intercellular CO2 concentration (Ci), and rubisco enzyme activity decreased, resulting in reduced CO2 supply and assimilation, thereby suppressing dark reactions. The biggest photochemical efficiency (Fv/Fm) and PSⅡ potential activity (Fv/F0) of leaves did not change significantly,indicating that the ability of using solar power and potential activity of PSⅡwas not affected. Among the osmoregulatory substances, proline and soluble sugar were important regulatory substances for plant to resist phenanthrene stress. The results would provide the basis for revealing the toxicity mechanism of PAHs on Salix viminalis and strengthening the research on plant environmental restoration.

Key words: Salix viminalis, phenanthrene, photosynthetic characteristics, osmoregulatory substance

摘要: 为研究菲对蒿柳生理生化的影响,以蒿柳扦插苗为试验材料,采用水培的方式,测定不同浓度(0.2、0.5、1.0和1.2 mg·L-1)菲对蒿柳株高及生理的影响,并选取对植物生长有显著抑制作用的最低浓度(1.0 mg·L-1)菲对蒿柳进行16 d的处理,测定其光合特性及渗透调节物质的变化。结果表明,高浓度菲处理显著抑制了蒿柳的株高和根系活力,使其电导率升高,膜透性增大。1.0 mg·L-1菲胁迫下,蒿柳的光合作用受到抑制,光化学淬灭系数(qP)显著下降,光反应受到影响;气孔导度(Gs)和胞间CO2浓度(Ci)下降、Rubisco活性降低,导致CO2供应和同化减少,暗反应受到抑制。叶片的最大光化学效率(Fv/Fm)和PSⅡ潜在活性(Fv/F0)无显著变化,说明PSⅡ利用光能的能力和潜在活性未受到影响。渗透调节物质中,脯氨酸和可溶性糖是植物抵御菲胁迫的重要调节物质。本研究结果为揭示多环芳烃(PAHs)对蒿柳产生的毒害机制及加强植物对环境的修复研究提供了依据。

关键词: 蒿柳, 菲, 光合特性, 渗透调节物质