Optimization of the Extraction Process of Soluble Protein From Peanut Leaf by Response Surface Methodology and Its Antioxidant Activity

doi:10.11869/j.issn.100-8551.2020.04.0796

Journal of Nuclear Agricultural Sciences ›› 2020, Vol. 34 ›› Issue (4): 796-804.DOI: 10.11869/j.issn.100-8551.2020.04.0796

• FoodIrradiation·FoodScience • Previous Articles Next Articles

Optimization of the Extraction Process of Soluble Protein From Peanut Leaf by Response Surface Methodology and Its Antioxidant Activity

GAO Qi^1,2, LIU Ziheng¹, GENG Yi¹, JIN Xiaoyi¹, WU Jiale¹, LU Yating¹, ZHANG Qian¹, XUE Youlin^1,*

1 College of Light Industry, Liaoning University, Shenyang, Liaoning 110036;
2 Party School of Liaoning Provincial Party Committee, Shenyang, Liaoning 110161

Received:2019-01-02 Online:2020-04-10 Published:2020-02-12

响应面优化花生叶可溶性蛋白提取工艺及抗氧化活性分析

高琦^1,2, 刘梓蘅¹, 耿艺¹, 金小乂¹, 吴嘉乐¹, 卢雅婷¹, 张倩¹, 薛友林^1,*

1 辽宁大学轻型产业学院,辽宁沈阳 110036;
2 中共辽宁省委党校, 辽宁沈阳 110161

通讯作者: ^*薛友林,男,副教授,主要从事农产品加工研究。E-mail:xueyoulin@lnu.edu.cn
作者简介:高琦,女,讲师,主要从事农产品加工研究。E-mail:gaoqi0925@163.com
基金资助:
辽宁省“兴辽英才计划”项目(XLYC1807270),辽宁省教育厅科学技术研究服务地方项目(LFW201704),辽宁大学大学生创新创业训练计划项目(X201810140247、X201810140252、X201810140253、X201810140257)

Abstract

Abstract: The effects of extraction pH, solid-liquid ratio and extraction together with the alkalidissolution and acid precipitation method, time on the protein extraction rate were investigated for the extraction process of peanut soluble leaf proteins. Based on the results of single factor tests, the Box-Behnken design and response surface method (RSM) were applied to optimize the extraction rate and the antioxidant activities of peanut leaf soluble proteins were also investigated. The results showed that the order of factors affecting the extraction rate of peanut leaf proteins was pH>extraction time>solid-liquid ratio, and the optimal conditions were: pH9, liquid-solid ratio 1:20(w/v)and extraction time 40 min. The regression model obtained was highly significant (R²=0.992 7), showing excellent goodness-of-fit. The predicted peanut soluble leaf protein extraction rate was 52.8% and the actual protein extraction rate was 54.2% with 63.8% purity, which showed strong DPPH and superoxide anion radical scavenging abilities and good reducing power. The results of this study could provide a theoretical support for the development and utilization of peanut leaves and the extension of the value chain peanut industry.

Key words: peanut leaves protein, response surface method, extraction process, antioxidant

摘要： 为确定花生叶可溶性蛋白提取最佳工艺,本试验以花生叶为材料,利用碱溶酸沉法提取花生叶可溶性蛋白,分析浸提pH值、料液比及提取时间对蛋白提取率的影响;在单因素试验的基础上,采用Box-Behnken响应面分析法(RSM)优化花生叶可溶性蛋白提取条件,并对所得蛋白进行抗氧化活性分析。结果表明,各影响因素主次顺序为浸提pH值>提取时间>料液比,最佳提取条件为料液比1∶20(w/v)、提取时间40 min、浸提pH值 9.0,以此条件建立的响应面试验回归模型拟合性好(R²=0.992 7),花生叶可溶性蛋白理论提取率为52.8%,实际提取率为54.2%,纯度为63.8%。花生叶可溶性蛋白具有较强的DPPH自由基、超氧阴离子自由基清除能力,以及较好的还原能力。本研究结果为花生叶的开发利用及花生产业链的延长提供了一定的数据支持。

关键词: 花生叶蛋白, 响应面法, 提取工艺, 抗氧化

GAO Qi, LIU Ziheng, GENG Yi, JIN Xiaoyi, WU Jiale, LU Yating, ZHANG Qian, XUE Youlin. Optimization of the Extraction Process of Soluble Protein From Peanut Leaf by Response Surface Methodology and Its Antioxidant Activity[J]. Journal of Nuclear Agricultural Sciences, 2020, 34(4): 796-804.

高琦, 刘梓蘅, 耿艺, 金小乂, 吴嘉乐, 卢雅婷, 张倩, 薛友林. 响应面优化花生叶可溶性蛋白提取工艺及抗氧化活性分析[J]. 核农学报, 2020, 34(4): 796-804.

References

[1] 施曼, 高岩, 易能, 张维国, 严少华. 植物叶蛋白提取及脱色研究进展[J]. 食品工业科技, 2017, 38(21):342-346, 351
[2] 柳青海, 张唐伟, 李天才. 叶蛋白提取分离及应用研究进展[J]. 食品工业科技, 2011, 32(9):468-471
[3] 王震, 乔天磊, 霍乃蕊, 高文伟. 植物叶蛋白提取及应用前景[J]. 山西农业科学, 2015, 43(12):1727-1731
[4] 刘娟, 汤丰收, 张俊, 臧秀旺, 董文召, 易明林, 郝西. 国内花生生产技术现状及发展趋势研究[J]. 中国农学通报, 2017, 33(22):13-18
[5] 金小乂. 富硒花生秧蛋白的提究取优化及抗氧化活性研究[D]. 沈阳:辽宁大学, 2017
[6] Katam R, Sakata K, Suravajhala P, Pechan T, M. Kambiranda D, Naik K S, Guo B Z, Basha S M. Comparative leaf proteomics of drought-tolerant and -susceptible peanut in response to water stress[J]. Journal of Proteomics, 2016, 143:209-226
[7] Chen N, Yang Q L, Hu D Q, Pan L J, Chi X Y, Chen M N, Yang Z, Wang T, Wang M, He Y N, Yu S L.Gene expression profiling and identification of resistance genes to low temperature in leaves of peanut (Arachis hypogaea L. )[J]. Scientia Horticulturae, 2014, 169:214-225
[8] Cossetin J F, Brum E S, Casoti R, Camponogara C, Dornelles R C, Maziero M, Antoniazzi C T D, Guex C G, Ramos A P, Ramos A P, Engelmann A M, Andrade C M, Manfron M P, Oliveira S M, Bauermann L F, Sagrillo M R, Machado A K, Santos A R S, Trevisan G. Peanut leaf extract has antioxidant and anti-inflammatory activity but no acute toxic effects[J]. Regulatory Toxicology and Pharmacology, 2019, 107: 104407
[9] Gao Q, Liu Z H, Wu J L, Geng Y, Zhang Q, Tie M, Gu X J, Tanokuar M, Xue Y L.Foliar application is an effective method for incorporating selenium into peanut leaf proteins with antioxidant activities[J]. Food Research International, 2019, 126: 108617
[10] Yang X, Li Y L, Li S Y, Oladejo A O, Wang Y C, Huang S F, Zhou C S, Ye X F, Ma H L, Duan Y Q.Effects of ultrasound-assisted α-amylase degradation treatment with multiple modes on the extraction of rice protein[J]. Ultrasonics sonochemistry, 2018, 40: 890-899
[11] Tenorio A T, Gieteling J, De Jong G A H, Boom R M, van derGoot A J. Recovery of protein from green leaves: Overview of crucial steps for utilisation[J]. Food Chemistry, 2016, 203: 402-408
[12] Ngoh Y Y, Gan C Y.Enzyme-assisted extraction and identification of antioxidative and α-amylase inhibitory peptides from Pinto beans (Phaseolus vulgaris cv. Pinto)[J]. Food Chemistry, 2016, 190: 331-337
[13] 李加兴, 向东, 周炎辉, 黄诚. 碱提酸沉法提取黄秋葵籽蛋白的工艺条件优化[J]. 食品科学, 2013, 34(20):23-26
[14] Hou F R, Ding W H, Qu W J, Oladejo A O, Xiong F, Zhang W W, He R H, Ma H L.Alkali solution extraction of rice residue protein isolates: Influence of alkali concentration on protein functional, structural properties and lysinoalanine formation[J]. Food Chemistry, 2017, 218: 207-215
[15] 中华人民共和国国家卫生和计划生育委员会, 国家食品药品监督管理总局. GB 5009.5-2016 食品安全国家标准食品中蛋白质的测定[S]. 北京:中国标准出版社, 2016
[16] 张晶晶, 李萧, 姚广龙, 曹献英, 陈健. 响应面法优化腰果蛋白提取工艺[J]. 食品工业科技, 2016, 37(22):305-308, 314
[17] 张小林, 陈博文, 孙晓瑞, 骆燕, 杜红霞, 孟俊龙, 常明昌, 徐丽婧. 响应面法优化超声波辅助灵芝黑色素提取工艺[J]. 核农学报, 2018, 32(3):532-538
[18] 马武生, 孙长花, 张占军, 王君, 蔡长春, 张夏华. 响应面法优化可溶性花生粕蛋白提取工艺[J]. 食品研究与开发, 2017, 38(21):39-45
[19] 邓祥元, 成婕, 刘孟姣, 高坤, 崔思宇. 响应面法优化小球藻叶绿素提取工艺及其稳定性研究[J]. 东北农业大学学报, 2015, 46(7):40-49
[20] 赵月, 高琦, 王晓文, 贾有峰, 张俊伟, 艾辛梓, 薛友林. 响应面试验优化碱溶酸沉法提取山药储藏蛋白工艺[J]. 食品科学, 2015, 36(16):7-11
[21] Czyzowska A, Klewicka E, Pogorzelski E, Nowak A.Polyphenols, vitamin C and antioxidant activity in wines from Rosa canina L. and Rosa rugosa Thunb[J]. Journal of Food Composition and Analysis, 2015, 39: 62-68
[22] 黄越, 周春晖, 黄惠华. 不同提取方法猴头菇粗多糖的表征及其抗氧化活性的比较[J]. 食品工业科技, 2017, 38(3):80-86
[23] 陈慧, 盛丹丹, 王文君. 黄金茶醇提取物成分及抗氧化活性的研究[J]. 现代食品科技, 2017, 33(1):26-32
[24] Choudhary A, Kumar R, Srivastava R B, Surapaneni S K, Tikoo K, Singh Ⅰ P.Isolation and characterization of phenolic compounds from Rhodiola imbricata, a Trans-Himalayan food crop having antioxidant and anticancer potential[J]. Journal of Functional Foods, 2015, 16: 183-193
[25] 黄浩, 秦高一鑫, 陈贵堂, 綦国红, 程抒劼, 杨志萍. 响应面法优化黄芪下脚料蛋白提取工艺[J]. 食品工业科技, 2017, 38(23):170-176
[26] 谢蓝华, 陈佳, 张淑谊, 林茂森, 陈云峰, 陈军, 杜冰. 美藤果蛋白的提取工艺及氨基酸组成分析[J]. 中国油脂, 2017, 42(5):40-44
[27] 周茜, 韩雪, 韩晓梅, 闫晨静, 白冰瑶, 王唯霖, 赵文. 响应面试验优化乌梅熊果酸提取工艺及其对大肠杆菌的抑制作用[J]. 食品科学, 2016, 37(8):67-73
[28] 洪晶, 陈涛涛, 唐梦茹, 汪少芸. 响应面法优化韭菜籽蛋白质提取工艺[J]. 中国食品学报, 2013, 13(12):89-96
[29] Cui Q Y, Ni X H, Zeng L, Tu Z, Li J, Sun K, Chen X, Li X H.Optimization of protein extraction and decoloration conditions for tea residues[J]. Horticultural Plant Journal, 2017, 3(4): 172-176
[30] 吴桂玲, 刘立品, 李文浩, 张伟, 刘晓雪, 张国权. 碱溶酸沉法提取青稞蛋白质的工艺研究[J]. 食品研究与开发, 2015, 36(5):19-24
[31] 于士婷, 尹翌秋, 孙天霞, 张丽轩, 王敏, 赵大庆, 王思明. 响应面法优化枸杞水溶性蛋白提取工艺[J]. 食品研究与开发, 2017, 38(9):79-84
[32] 李佳, 徐慧, 刘建军, 李博, 高淑娟. 响应面法优化芝麻蛋白的提取工艺[J]. 中国酿造, 2016, 35(4):140-144
[33] 赵节昌. 响应面法优化酸枣仁蛋白提取工艺[J]. 食品科学, 2013, 34(16):134-138
[34] 郭志华, 曹稳根, 廖红艳. 叶蛋白的研究进展[J]. 宿州学院学报, 2007(6):113-115
[35] 王震, 乔天磊, 霍乃蕊, 高文伟. 植物叶蛋白提取方法及研究进展[J]. 山西农业科学, 2016, 44(1):126-130
[36] Ningappa M B, Srinivas L.Purification and characterization of~35 kDa antioxidant protein from curry leaves (Murraya koenigii L. )[J]. Toxicology in Vitro, 2008, 22(3):699-709
[37] 孙朦, 王鸿飞, 李艳霞, 王凯凯, 宋佳敏, 许凤, 邵兴锋, 李和生. 辣木叶总黄酮提取工艺优化及抗氧化能力研究[J]. 核农学报, 2018, 32(9):1772-1780
[38] 陶阿丽, 冯学花, 曹殿洁, 华芳, 杨梅兰. 豆腐柴叶蛋白闪式提取工艺优化计及其体外抗氧化活性研究[J]. 长江大学学报(自然科学版), 2018, 15(10):50-56, 6
[39] 文静, 解新安, 李雁, 李璐. 微波辅助碱法提取茶渣蛋白工艺及抗氧化活性研究[J]. 粮食与油脂, 2017, 30(5):41-44
[40] 赵立娜, 汪少芸, 饶平凡, 江勇, 黄家炽, 周绍迁. 茶渣蛋白的提取、有限酶解及抗氧化性质研究[J]. 中国食品学报, 2012, 12(2):53-60
[41] 韩雅利, 高丹丹, 马洪鑫, 余琼, 李明生. 苜蓿蛋白抗氧化肽的研究[J]. 农产品加工, 2018(23):15-18

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创刊：1987年
主管部门：农业农村部
主办单位：中国原子能农学会与中国农业科学院农产品加工研究所（前中国农业科学院原子能利用研究所）
ISSN：1000-8551

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Optimization of the Extraction Process of Soluble Protein From Peanut Leaf by Response Surface Methodology and Its Antioxidant Activity

响应面优化花生叶可溶性蛋白提取工艺及抗氧化活性分析

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