Changes in Microbial Community Functional Diversity in Soil Aggregate of Black Soil Turned From Upland Into Paddy Tillage in Northeast China

doi:10.11869/j.issn.100-8551.2020.12.2831

Journal of Nuclear Agricultural Sciences ›› 2020, Vol. 34 ›› Issue (12): 2831-2838.DOI: 10.11869/j.issn.100-8551.2020.12.2831

• Isotope Tracer Technique·Ecology and Environment·Physiology • Previous Articles Next Articles

Changes in Microbial Community Functional Diversity in Soil Aggregate of Black Soil Turned From Upland Into Paddy Tillage in Northeast China

ZHAO Meichun¹, LI Xin², WANG Yue¹, ZHAO Hancheng³, ZHAO Dongxue¹, WANG Panpan¹, SUN Guangyu^1,*

¹College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang 150000;
²College of Resources and Environment, Northeast Agricultural University, Harbin, Heilongjiang 150000;
³State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, Heilongjiang 150000

Received:2019-05-13 Online:2020-12-10 Published:2020-10-13

东北黑土旱田改稻田对土壤团聚体微生物群落功能多样性的影响

赵美纯¹, 李鑫², 王月¹, 赵汉丞³, 赵冬雪¹, 王盼盼¹, 孙广玉^1,*

¹东北林业大学生命科学学院,黑龙江哈尔滨 150000;
²东北农业大学资源与环境学院,黑龙江哈尔滨 150000;
³东北林业大学林木遗传育种国家重点实验室,黑龙江哈尔滨 150000

通讯作者: ^*孙广玉,男,教授,主要从事植物营养生理研究。E-mail:sungy@nefu.edu.cn
作者简介:赵美纯,女,主要从事植物营养生理研究。E-mail:zmcsm2017@126.com
基金资助:
国家自然科学基金(41701289)

Abstract

Abstract: In order to understand the changes of microbial community functional diversity in the soil aggregates after turning from upland into paddy tillage in black soil region of Northeast China, taking the black soil turned from upland (mainly planting corn) into paddy tillage (abbreviated as “land to paddy”) in Northeast China as a study object, the changes in microbial diversity index and the ability of carbon source utilization in all soil aggregates after “land to paddy” were investigated by using the Biolog-Eco microplate technique. The results showed that there was significant increased in the soil water-stable macro-aggregates number after “land to paddy” increased the content of soil organic carbon (SOC) in all soil aggregates. The average colour change rate of soil microbial community in all soil aggregates of different grain sizes in upland and paddy fields (AWCD) followed the order of upland silt + clay > upland macro-aggregates > upland micro-aggregates > paddy tillage macro-aggregates > paddy tillage silt + clay > paddy tillage micro-aggregates. The microbial diversity index and the ability of carbon source utilization in all soil aggregates obviously decreased by “land to paddy”, especially the carbon source of amino acids and carbohydrates. Results of principal component analysis showed that the function of carbon source utilization in all soil aggregates in upland and paddy tillage had changed. In summary, “land to paddy” obviously changed the microbial functional diversity in soil aggregate of black soil, and decreased the ability of carbon source utilization for soil microbial metabolic activity. This study provides a theoretical reference for the sustainable land utilization of Northeast China.

Key words: black soil, soil aggregate microorganisms, diversity, upland, paddy tillage

摘要： 为了解东北黑土旱田改种稻田后土壤团聚体中微生物群落功能多样性的变化,将东北黑土旱田(种植玉米为主)改种为稻田(简称“旱改稻”),采用Biolog-Eco微平板技术,研究了“旱改稻”后不同粒径土壤团聚体微生物的多样性指数以及其对碳源利用能力的变化。结果表明,“旱改稻”显著增加了黑土土壤水稳性微团聚体数量,增加了不同粒径土壤团聚体内有机碳(SOC)的含量。旱田和稻田不同粒径土壤团聚体内的土壤微生物群落平均颜色变化率(AWCD)表现为旱田淤泥+粘土>旱田大团聚体>旱田微团聚体>稻田大团聚体>稻田淤泥+粘土>稻田微团聚体。“旱改稻”明显降低了各粒径土壤团聚体微生物的多样性指数以及碳源利用能力,尤其对氨基酸与碳水化合物最为明显。主成分分析结果表明,旱田和稻田不同粒径土壤团聚体微生物群落功能多样性差异显著。综上,“旱改稻”明显改变了黑土土壤团聚体微生物群落功能多样性,降低了以碳源为底物的土壤微生物代谢活性。本研究结果为东北黑土区土壤可持续利用提供了理论参考。

关键词: 黑土, 土壤团聚体微生物, 多样性, 旱田, 稻田

ZHAO Meichun, LI Xin, WANG Yue, ZHAO Hancheng, ZHAO Dongxue, WANG Panpan, SUN Guangyu. Changes in Microbial Community Functional Diversity in Soil Aggregate of Black Soil Turned From Upland Into Paddy Tillage in Northeast China[J]. Journal of Nuclear Agricultural Sciences, 2020, 34(12): 2831-2838.

赵美纯, 李鑫, 王月, 赵汉丞, 赵冬雪, 王盼盼, 孙广玉. 东北黑土旱田改稻田对土壤团聚体微生物群落功能多样性的影响[J]. 核农学报, 2020, 34(12): 2831-2838.

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创刊：1987年
主管部门：农业农村部
主办单位：中国原子能农学会与中国农业科学院农产品加工研究所（前中国农业科学院原子能利用研究所）
ISSN：1000-8551

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Changes in Microbial Community Functional Diversity in Soil Aggregate of Black Soil Turned From Upland Into Paddy Tillage in Northeast China

东北黑土旱田改稻田对土壤团聚体微生物群落功能多样性的影响

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