Bioinformatics and Expression Analysis of Alternative Oxidase Genes in Sweetpotato

doi:10.11869/j.issn.100-8551.2022.02.0270

Journal of Nuclear Agricultural Sciences ›› 2022, Vol. 36 ›› Issue (2): 270-281.DOI: 10.11869/j.issn.100-8551.2022.02.0270

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles Next Articles

Bioinformatics and Expression Analysis of Alternative Oxidase Genes in Sweetpotato

ZHOU Shuqian¹(), CHEN Lu¹, CHEN Huiyun², LI Yongxin¹, CHEN Gang¹, LU Guoquan¹, YANG Huqing¹^,^*()

¹School of Argriculture and Food Science, Zhejiang Agriculture & Forestry University, Lin'an,Zhejiang 311300
²Agricultural Products Processing Research Institute, Ningbo Academy of Agricultural Sciences, Ningbo, Zhejiang 315040

Received:2020-12-30 Accepted:2021-02-08 Online:2022-02-10 Published:2022-01-17
Contact: YANG Huqing

甘薯交替氧化酶基因家族生物信息学与表达分析

周淑倩¹(), 陈璐¹, 陈惠云², 李永新¹, 陈刚¹, 陆国权¹, 杨虎清¹^,^*()

¹浙江农林大学农业与食品科学学院,浙江临安 311300
²宁波市农业科学研究院农产品加工研究所,浙江宁波 315040

通讯作者: 杨虎清
作者简介:周淑倩,女,主要从事农产品质量与安全控制研究。E-mail: 873306241@qq.com
基金资助:
宁波市公益性计划重点项目(202002N3081);国家自然科学基金项目(31871857);浙江省自然科学基金项目(LQ20C200002)

Abstract

Abstract:

This study was aimed to identify the members of alternative oxidase (AOX) gene family in sweetpotato [Ipomoea batatas (L.) Lam.], and characterize their expression patterns in response to biotic and abiotic stresses. Based on genome wide screening and gene cloning and sequencing, three members of AOX gene family were obtained from Xinxiang sweetpotato. The gene structure analysis showed that the cDNA of three AOX genes in sweetpotato were 963 bp, 1080 bp and 1032 bp in length, encoded 320, 359 and 343 amino acids, respectively. Three AOX genes all contained 4 exons and 3 introns, and were named as IbAOX1a (GenBank accession number:MT992313), IbAOX1b (GenBank accession number:MT992314) and IbAOX2 (GenBank accession number:MG450566.1), respectively. Protein homology analysis showed that the protein sequences of IbAOX were very conservative at the C-terminus, only minor differences existed at the N-terminus, and there were special Fe-binding conserved domains of EXXH, FXHR and EEE-Y in the sequences. Subcellular localization prediction showed that IbAOX were mainly located in mitochondria. Protein secondary structure prediction showed that the primary secondary structure of the gene family was α-helix, β-turn, extended chain and irregular coils. Real-time fluorescence quantitative PCR (qRT-PCR) analysis showed that the expression of IbAOXs gene was tissue-specific. The transcript level of IbAOX1a in leaves was the highest, while IbAOX1b and IbAOX2 were highly expressed in tuberous roots. The expression of IbAOX1a and IbAOX2 in buds were hardly detected. The expression of IbAOX1a and IbAOX1b in seedlings was stimulated under cold, drought and salt stresses. The transcript level of IbAOX1a was significantly higher than that of IbAOX1b in tuberous roots at 4℃, while the expression IbAOX1b in tuberous roots infected by Fusarium solani was markedly higher than that of IbAOX1a. However, the expression of IbAOX2 in sweetpotato seedlings or tuberous root was not altered under various stresses, showing a constitutive expression pattern. This study provides a theoretical basis for studying the functions of AOX genes and breeding sweetpotato varieties with stress resistances.

Key words: sweetpotato, alterative oxidase, bioinformatics analysis, expression analysis, stress

摘要：

为分离甘薯[Ipomoea batatas (L.) Lam.]交替氧化酶(AOX)基因家族成员,分析其在甘薯应答生物和非生物胁迫中的响应模式,本试验在筛选甘薯基因组的基础上,进行基因克隆和测序,最终从心香甘薯中获得3个AOX基因家族成员。基因结构分析结果表明,3个甘薯AOX基因的cDNA全长依次为963、1 080 和1 032 bp,分别编码320、359和343个氨基酸,均含4个外显子和3个内含子,将其分别命名为IbAOX1a(GenBank登录号:MT992313)、IbAOX1b(GenBank登录号:MT992314)和IbAOX2 (GenBank登录号:MG450566.1)。蛋白同源性分析结果显示,甘薯AOX蛋白序列在C端十分保守,仅在N端有较小差异,且序列中都存在特有的EXXH、FXHR和EEE-Y铁离子结合保守结构域。亚细胞定位预测结果表明该基因家族成员主要定位在线粒体。蛋白质二级结构预测表明,该基因家族成员主要以α-螺旋、β-转角、延伸链和不规则卷曲为主。实时荧光定量PCR(qRT-PCR)分析结果表明,IbAOXs基因的表达具有组织特异性,IbAOX1a在叶片中表达最高,IbAOX1b和IbAOX2在块根中高表达,IbAOX1a和IbAOX2在花蕾中几乎不表达。IbAOX1a和IbAOX1b在低温、干旱和盐胁迫的幼苗中均表达上调。IbAOX1a在低温块根中的表达高峰显著高于IbAOX1b,而在腐皮镰刀菌(Fusarium solani)侵染的块根中IbAOX1b的表达量显著高于IbAOX1a。无论在甘薯幼苗或块根中,IbAOX2对各种胁迫都没有响应,呈现组成型表达模式。本研究为甘薯AOX基因的功能挖掘及甘薯抗逆品种筛选提供了一定的理论依据。

关键词: 甘薯, 交替氧化酶, 生物信息学分析, 表达分析, 胁迫

ZHOU Shuqian, CHEN Lu, CHEN Huiyun, LI Yongxin, CHEN Gang, LU Guoquan, YANG Huqing. Bioinformatics and Expression Analysis of Alternative Oxidase Genes in Sweetpotato[J]. Journal of Nuclear Agricultural Sciences, 2022, 36(2): 270-281.

周淑倩, 陈璐, 陈惠云, 李永新, 陈刚, 陆国权, 杨虎清. 甘薯交替氧化酶基因家族生物信息学与表达分析[J]. 核农学报, 2022, 36(2): 270-281.

Figures/Tables 15

Table 1 Full-length primers of AOX gene family in sweetpotato

基因名称 Gene name	引物序列(5'→3') Primer sequence(5'→3')	产物大小 Product size/bp
IbAOX1a	F:ATGAGTCATAACGGGGCG R:CTAATGATACCCAATTGGAGCA	963
IbAOX1b	F:ATGATGAGCCGTGGCG R:TTAGTGATATCCAAGAGGGGC	1 080
IbAOX2	F:ATGAATCAGTTGCTGGCTAG R:TCAGTGGTACCCAATAGGA	1 040

Table 2 qRT-PCR primers for AOX gene family in sweetpotato

基因名称 Gene name	引物序列(5'→3') Primer sequence(5'→3')	产物大小 Product size/bp
IbAOX1a	F:TCCTGGAAGAAGCGGAGAAC R:GGTGAAGAAATAGGCGTTGAA	128
IbAOX1b	F:AGTGCTGGTTGCTTGGATGA R:CCATTCCGTGCCATCCTCTT	199
IbAOX2	F:GTTGCCTCCAATGATTACCA R:AACTCTCGTGAATAGCCCTCA	93

Fig.1 PCR amplification of IbAOXs gene and pMD19-T-IbAOXs recombinant vector Note: A: M: Marker. 1: IbAOX1a. 2: IbAOX1b. 3: IbAOX2. B: M: Marker. 1: PMD19-T-IbAOX1a recombinant carrier bacterial solution.2: PMD19-T-IbAOX1b recombinant carrier bacterial solution. 3: PMD19-T-IbAOX2 recombinant carrier bacterial solution.

Fig.2 Structure analysis of AOX genes from sweetpotato

Table 3 Basic physicochemical characteristics and subcellular localization prediction of AOX gene family in sweetpotato

基因名称 Gene name	编码区序列长度 Coding sequence length/bp	蛋白长度 Protein length/aa	分子式 Formula	分子量 Molecular weight/Da	等电点 pI	不稳定指数 Instability index	脂溶指数 Aliphatic index	总平均亲水性 GRAVY	亚细胞定位 Subcellular localization
IbAOX1a	963	320	C₁₆₄₉H₂₅₀₀ N₄₄₈O₄₅₈S₁₁	36 281.38	8.24	42.25	78.12	-0.290	线粒体
IbAOX1b	1 080	359	C₁₈₁₅H₂₇₉₄ N₅₀₈O₅₀₇S₁₆	40 356.21	8.79	43.75	75.29	-0.292	线粒体
IbAOX2	1 032	343	C₁₇₅₅H₂₇₅₁ N₄₈₇O₄₈₉S₂₃	39 234.50	8.91	39.07	82.48	-0.206	线粒体

Fig.3 Hydrophilic and hydrophobic analysis of AOX protein in sweetpotato

Fig.4 Multiple sequence alignment of AOX protein in sweetpotato Note: The color from dark to light represents 100%, 75%, 50% and less than 50% homology. IbAOXs: Ipomoea batatas (MT992313, MT992314, MG450566.1). AtAOXs: Arabidopsis thaliana (NP_188876.1, NP_188871.1, NP_189399.1, NP_564395.1, AAY78882.1). SlAOXs: Solanum lycopersicum(NP_001234117.2, NP_001234120.1, NP_001309890.1). DhAOX: Dorcoceras hygrometricum (KZV31497.1).

Fig.5 Phylogenetic analysis of AOX protein in sweetpotato

Fig.6 Analysis of AOX protein Motif1~Motif20 in sweetpotato

Table 4 Conserved sequence of AOX protein in sweetpotato

Motif	长度 Length/bp	蛋白保守序列 Protein conserved sequence
Motif1	50	WKWNCFRPWETYKADVSIDLKKHHVPKTFMDKFAYWTVKALRFPTDIFFQ
Motif2	50	PGMVGGMLLHCKSLRRFEHSGGWIKALLEEAENERMHLMTFIELSKPRWY
Motif3	50	IENIPAPAIAIDYWRLPPDARLKDVITVIRADEAHHRDVNHFASDIRFQG
Motif4	50	LVFAVQGVFFNAYFVAYLASPKLAHRIVGYLEEEAIHSYTEFLNDIDNGK
Motif5	15	RRYGCRAMMLETVAA
Motif6	18	VSSYWGVDPPKVSKEDGS
Motif7	11	LKEAPAPIGYH
Motif8	10	ZKEKDEKNGG
Motif9	7	WLDEPVM
Motif10	6	PRIFSV
Motif11	6	SRRMMS
Motif12	7	MSRGCCR
Motif13	6	GEGGIG
Motif14	6	HGGPVK
Motif15	10	NQAAGEAVDR
Motif16	6	RRWATA
Motif17	6	SRYDSA
Motif18	6	RSLYGN
Motif19	6	ASEEKK
Motif20	7	SGTLLRK

Table 5 Analysis of the secondary structure of AOX proteins in sweetpotato/%

蛋白名称 Protein name	α-螺旋 Alpha helix	延伸链 Extended strand	β-转角 Beta turn	不规则卷曲 Random coil
IbAOX1a	47.19	14.06	7.50	31.25
IbAOX1b	46.80	12.53	5.85	34.82
IbAOX2	60.64	7.00	4.57	27.99

Fig.7 Prediction of the secondary and tertiary structure of AOX protein in sweetpotato

Fig.8 Expression analysis of IbAOXs genes in tuberous roots, fibrous roots, stems, leaves and buds of sweetpotato Note: Different lowercase letters indicate significant differences at 0.05 level.

Fig.9 Analysis of IbAOXs gene expression in sweetpotato seedlings in response to low temperature drought and high salt stress Note: The difference between the two points is greater than LSD indicate significant differences at 0.05 level. The same as following.

Fig.10 Analysis of IbAOXs gene in tuberous roots in response to low temperature and F. solani

References 39

[1]	张立新, 冯志宏, 王春生. 影响红薯贮藏保鲜的因素及其保鲜技术[J]. 保鲜与加工, 2010, 10(4):51-54
[2]	Saha B, Borovskii G, Panda S K. Alternative oxidase and plant stress tolerance[J]. Plant Signaling and Behavior, 2016, 11(12):1-11
[3]	Borecky J, Nogueira F T S, De Oliveira K A P, Maia I G, Vercesi A E, Arruda P. The plant energy-dissipating mitochondrial systems: Depicting the genomic structure and the expression profiles of the gene families of uncoupling protein and alternative oxidase in monocots and dicots[J]. Journal of Experimental Botany, 2006, 57(4):849-864 PMID
[4]	Wanniarachchi V R, Dametto L, Sweetman C, Shavrukov Y, Day D A, Jenkins C L D, Soole K L. Alternative respiratory pathway component genes (AOX and ND) in rice and barley and their response to stress[J]. International Journal of Molecular Sciences, 2018, 19(3):915-936 DOI URL
[5]	Brew-Appiah R A T, York Z B, Krishnan V, Roalson E H, Sanguinet K A. Genome-wide identification and analysis of the ALTERNATIVE OXIDASE gene family in diploid and hexaploid wheat[J]. PLoS One, 2018, 13(8):1-43
[6]	Vanlerberghe G C, McIntosh L. Lower growth temperature increases alternative pathway capacity and alternative oxidase protein in tobacco[J]. Plant Physiology, 1992, 100(1):115-119 PMID
[7]	敬媛媛, 阿塔吾拉·铁木尔, 胡江伟,. 魏佳, 张平, 吴斌. 外源NO对伽师瓜AOX基因克隆表达及抗氰呼吸的影响[J]. 食品工业科技, 2017, 38(16):296-302
[8]	刘慧春, 田丹青, 刘建新, 马广莹, 邹清成, 朱祝军. 红掌交替氧化酶基因克隆及其在低温胁迫下的表达分析[J]. 核农学报, 2013, 27(10):1464-1472
[9]	Mróz T L, Havey M J, Bartoszewski G. Cucumber possesses a single terminal alternative oxidase gene that is upregulated by cold stress and in the mosaic (MSC) mitochondrial mutants[J]. Plant Molecular Biology Reporter, 2015, 33(6):1893-1906 DOI URL
[10]	Sweetman C, Soole K L, Jenkins C L D, Day D A. Genomic structure and expression of alternative oxidase genes in legumes[J]. Plant, Cell and Environment, 2019, 42(1):71-84 DOI
[11]	宗亚仙, 郝自远, 王曦, 温少莹, 李火根. 鹅掌楸AOX家族基因克隆与组织表达分析[J]. 广西植物, 2020, 40(7):988-997
[12]	Vanlerberghe G C. Alternative oxidase: A mitochondrial respiratory pathway to maintain metabolic and signaling homeostasis during abiotic and biotic stress in plants[J]. International Journal of Molecular Sciences, 2013, 14(4):6805-6847 DOI PMID
[13]	Wang J, Rajakulendran N, Amirsadeghi S, Vanlerberghe G C. Impact of mitochondrial alternative oxidase expression on the response of Nicotiana tabacum to cold temperature[J]. Physiologia Plantarum, 2011, 142(4):339-351 DOI URL
[14]	郝候艳. 拟南芥AOX的功能分析[D]. 西安: 西北大学, 2019
[15]	Li C R, Liang D D, Xu R F, Li H, Zhang Y P, Qin R Y, Li L, Wei P C, Yang J B. Overexpression of an alternative oxidase gene, OsAOX1a, improves cold tolerance in Oryza sativa L[J]. Genetics and Molecular Research, 2013, 12(4):5424-5432 DOI PMID
[16]	Murik O, Tirichine L, Prihoda J, Thomas Y, Araújo WLAllen A E, Fernie A R, Bowler C. Downregulation of mitochondrial alternative oxidase affects chloroplast function, redox status and stress response in a marine diatom[J]. New Phytologist, 2019, 221(3):1303-1316 DOI PMID
[17]	丁长庆. 西瓜交替氧化酶ClAOX及其相关基因ClCASPL在低温胁迫下的功能研究[D]. 杭州: 浙江大学, 2016
[18]	刘金亮, 王庆文, 冯汉青, 梁烨, 贾凌云. 盐和过氧化氢胁迫下交替氧化酶调节根生长和细胞死亡[J]. 西北大学学报(自然科学版), 2015, 45(6):974-978
[19]	Zhao C Z, Wang X M, Wang X, Wu K L, Li P, Chang N, Wang J F, Wang F, Li J L, Bi Y R. Glucose-6-phosphate dehydrogenase and alternative oxidase are involved in the cross tolerance of highland barley to salt stress and UV-B radiation[J]. Journal of Plant Physiology, 2015, 181:83-95 DOI URL
[20]	Sun Y J, Liu X H, Zhai H, Gao H Y, Yao Y X, Du Y P. Responses of photosystem Ⅱ photochemistry and the alternative oxidase pathway to heat stress in grape leaves[J]. Acta Physiologiae Plantarum, 2016, 38(10):232-237 DOI URL
[21]	Zalutskaya Z, Lapina T, Ermilova E. The chlamydomonas reinhardtii alternative oxidase 1 is regulated by heat stress[J]. Plant Physiology and Biochemistry, 2015, 97:229-234 DOI PMID
[22]	徐秀玉. 干旱胁迫下线粒体交替氧化酶途径在平邑甜茶光破坏防御中的作用[D]. 秦安: 山东农业大学, 2016
[23]	王庆文, 冯汉青, 戎富虎, 管冬冬, 孙坤, 贾凌云. 交替氧化酶对AlCl_3胁迫下烟草BY-2悬浮细胞死亡发生的影响[J]. 兰州大学学报(自然科学版), 2015, 51(4):564-568
[24]	Demircan N, Cucun G, Uzilday B. Mitochondrial alternative oxidase (AOX1a) is required for the mitigation of arsenic-induced oxidative stress in Arabidopsis thaliana[J]. Plant Biotechnology Reports, 2020, 14(2):235-245 DOI URL
[25]	Kumari A, Pathak P K, Bulle M, Igamberdiev A U, Gupta K J. Alternative oxidase is an important player in the regulation of nitric oxide levels under normoxic and hypoxic conditions in plants[J]. Journal of Experimental Botany, 2019, 70(17):4345-4354 DOI URL
[26]	Erdal S, Genisel M, Turk H, Dumlupinar R, Demir Y. Modulation of alternative oxidase to enhance tolerance against cold stress of chickpea by chemical treatments[J]. Journal of Plant Physiology, 2015, 175:95-101 DOI URL
[27]	梁五生, 徐婷, 姚飞, 李永红, 王政逸. 交替氧化酶可增强核盘菌对多菌灵的敏感性[J]. 中国农学通报, 2016, 32(6):193-197
[28]	Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.[J]. Molecular Biology and Evolution, 2011, 28(10):2731-2739 DOI URL
[29]	Livak K, Schmittgen T. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method[J]. Methods, 2001, 25(4):402-408 PMID
[30]	Considine M J, Holtzapffel R C, Day D A, Whelan J, Millar A H. Molecular distinction between alternative oxidase from monocots and dicots[J]. Plant Physiology, 2002, 129(3):949-953 PMID
[31]	Yang J, Moeinzadeh M H, Kuhl H, Helmuth J, Xiao P, Haas S, Liu G, Zheng J, Sun Z, Fan W, Deng G, Wang H, Hu F, Zhao S, Fernie A R, Boerno S, Timmermann B, Zhang P, Vingron M. Haplotype-resolved sweet potato genome traces back its hexaploidization history[J]. Nature Plants, 2017, 3(18):696-703. DOI URL
[32]	Fung R W M, Wang C Y, Smith D L, Gross K C, Tao Y, Tian M. Characterization of alternative oxidase (AOX) gene expression in response to methyl salicylate and methyl jasmonate pre-treatment and low temperature in tomatoes[J]. Journal of Plant Physiology, 2006, 163(10):1049-1060 DOI URL
[33]	Djajanegara I, Finnegan P, Mathieu C, McCabe T, Whelan J, Day D A. Regulation of alternative oxidase gene expression in soybean[J]. Plant Molecular Biology, 2002, 50:735-742 PMID
[34]	Saisho D, Nambara E, Naito S, Tsutsumi N, Hirai A, Nakazono M. Characterization of the gene family for alternative oxidase from Arabidopsis thaliana[J]. Plant Molecular Biology, 1997, 35(5):585-596 PMID
[35]	Feng H Q, Houa X L, Li X, Sun K, Wang R F, Zhang T G, Ding Y P. Cell death of rice roots under salt stress may be mediated by cyanide-resistant respiration[J]. Zeitschrift für Naturforschung C, 2014, 68(1/2):39-46 DOI URL
[36]	Feng H Q, Guan D D, Sun K, Wang Y F, Zhang T G, Wang R F. Expression and signal regulation of the alternative oxidase genes under abiotic stresses[J]. Journal of Biochemistry and Biophysics: English Edition, 2013, 46(12):985-994
[37]	Smith C A, Melino V J, Sweetman C, Soole K L. Manipulation of alternative oxidase can influence salt tolerance in Arabidopsis thaliana[J]. Physiologia Plantarum, 2009, 137(4):459-472 DOI URL
[38]	Chen H, Zhou S, Li X, Yang H. Exogenous progesterone alleviates chilling injury by upregulating IbAOX1 to mediate redox homeostasis and proline accumulation in postharvest sweetpotato tuberous root[J]. Postharvest Biology and Technology, 2022, 183:1-11
[39]	Zhang H, Zhou S, Pristijono P, Golding J B, Yang H, Chen G, Li Y. Role of AOX in low-temperature conditioning induced chilling tolerance in sweetpotato roots[J]. Scientia Horticulturae, 2021, 288:1-9

Metrics

Comments

创刊：1987年
主管部门：农业农村部
主办单位：中国原子能农学会与中国农业科学院农产品加工研究所（前中国农业科学院原子能利用研究所）
ISSN：1000-8551

访问统计
总访问：
今日访问：
当前在线：

Bioinformatics and Expression Analysis of Alternative Oxidase Genes in Sweetpotato

甘薯交替氧化酶基因家族生物信息学与表达分析

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 15

References 39

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YAO Rui, MA Xinlei, GU Pengpeng, LIN Xiaohu, GAO Hui. Genome Wide Identification and Expression Analysis of Thiolase Gene Family in Foxtail Millet(Setaria italica L.) [J]. Journal of Nuclear Agricultural Sciences, 2022, 36(2): 259-269.
[2]	HUANG Xin, FANG Yuanpeng, YUE Ningbo, ZHANG Long, WU Dan, LI Yunzhou. Identification of Double RNA Binding Protein(DRB)Gene Family and Analysis of Defense Response to TYLCV in Tomato [J]. Journal of Nuclear Agricultural Sciences, 2022, 36(2): 291-301.
[3]	ZHONG Huaiqin, KONG Lan, FAN Ronghui, FANG Nengyan, LIN Rongyan, LIN Bing. Cloning and Expression Analysis of Terpene Synthase Gene OnTPS From Oncidium Twinkle Red Fantasy [J]. Journal of Nuclear Agricultural Sciences, 2022, 36(2): 313-321.
[4]	BAN Wenhui, WANG Xingqiang, LIU Qiangjuan, SUN Jianbo, LYU Kaiyuan, KANG Jianhong. Effects of Nitrogen Application Rate on Potato Yield, Starch Content and Related Enzyme Metabolic During Tuber Formation After High Temperature Stress [J]. Journal of Nuclear Agricultural Sciences, 2022, 36(2): 422-434.
[5]	WANG Qingbin, LIU Zhiguo, PENG Chun'e, MENG Hui, ZHAO Hongling, WANG Hongfeng, ZHANG Min. Mechanism of Paecilomyces variotii Extract in Inducing Pakchoi Resistance to Low-Temperature Stress [J]. Journal of Nuclear Agricultural Sciences, 2022, 36(2): 473-480.
[6]	CAI Xiaoxi, SHEN Yang, HU Bingshuang, WANG Yan, CHEN Yue, SUN Mingzhe, JIA Bowei, SUN Xiaoli. Overexpression of A Glycine soja S-adenosylmethionine Synthetase GsSAMS in Rice Increases Salt-alkaline Tolerance [J]. Journal of Nuclear Agricultural Sciences, 2022, 36(1): 50-56.
[7]	XIAO Wenfei, CHAI Weiguo, QIU Jieren, XIN Ya, RUAN Songlin. Proteomic Analysis Reveals Mechanisms of Stropharia rugosoannulata Polysaccharides Promoting Kiwifruit Growth and High Temperature Resistance [J]. Journal of Nuclear Agricultural Sciences, 2022, 36(1): 94-104.
[8]	DENG Haodong, YU Meixia, WU Guangliang, LUO Xin, SONG Guiting, CHEN Liping, HE Haohua, BIAN Jianmin. The Detection of QTL Confers Cold Tolerance at the Bud Bursting Period in Rice Using CSSLs Population [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(9): 1971-1978.
[9]	LI Sen, WANG Qingjie, CHEN Xiude, ZHANG Rui, LI Ling, DU Guiying, FU Xiling. Cloning and Expression Analysis of Transcription Factor PpWRKY18 in Peach [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(9): 1987-1993.
[10]	TAN Zhengwei, LI Lei, YU Yongliang, XU Lanjie, YANG Hongqi, DONG Wei, MA Xinming, LIANG Huizhen. Cloning and Expression Analysis of S-adenosylmethionine Synthetase Gene From Carthamus tinctorius L. [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(9): 1994-2001.
[11]	FENG Yalan, YIN Fei, XU Ke, JIA Xiaoyi, ZHOU Shuang, MA Chao. Role of Sucrose Metabolism and Signal Transduction in Plant Development and Stress Response [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(9): 2044-2055.
[12]	ZHANG Shikai, WANG Min, CHENG Xinxin, ZHANG Qiyue, WU Peng. Ultrahigh Pressure Extraction of Polygonatum sibiricum Polysaccharide and Mechanism of Improving Exercise Endurance [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(9): 2094-2101.
[13]	YU Minglong, JIN Dan, LIU Meiling, LI Yao, ZHENG Dianfeng, FENG Naijie, LIANG Xilong. Effects of Prohexadione-Calcium on Root Growth and Physiological Responses of Different Salt-Tolerance Soybean Varieties Under Saline-Alkali Stress [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(9): 2154-2164.
[14]	YANG Min, LI Xiangling, HAN Jinling, YANG Qing, WANG Jian. Nicosulfuron Stress on Active Oxygen Accumulation, Antioxidant System and Related Gene Expression in Sweet Maize Seedlings [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(9): 2182-2193.
[15]	WEN Hongwei, YANG Bin, WANG Dongsheng. Research Progress on Promoting Growth and Drought Resistance of Wheat by Plant Growth Promoting Rhizobacteria [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(9): 2194-2203.