Journal of Nuclear Agricultural Sciences ›› 2022, Vol. 36 ›› Issue (1): 154-162.DOI: 10.11869/j.issn.100-8551.2022.01.0154

• Food Irradiation·Food Science • Previous Articles     Next Articles

The Bacterial Community Diversity of Traditional Fermented Yak Milk in Gannan of Gansu

MA Caixia1(), LIANG Qi1,*(), WANG Xiangzhu2, LIU Ying2   

  1. 1College of Food Science and Engineering, Gansu Agricultural University/Functional Dairy Product Engineering Laboratory of Gansu Province, Lanzhou, Gansu 730070
    2Gansu Linxia Liaoyuan Dairy Co., Ltd., Linxia, Gansu 731100
  • Received:2020-12-07 Accepted:2021-02-08 Online:2022-01-10 Published:2021-12-06
  • Contact: LIANG Qi

甘肃甘南传统发酵牦牛乳中细菌菌群的多样性研究

马彩霞1(), 梁琪1,*(), 王湘竹2, 刘瑛2   

  1. 1甘肃农业大学食品科学与工程学院/甘肃省功能乳品工程实验室,甘肃 兰州 730070
    2甘肃临夏州燎原乳业有限公司,甘肃 临夏 731100
  • 通讯作者: 梁琪
  • 作者简介:马彩霞,女,主要从事乳品微生物研究。E-mail: 280062500@qq.com
  • 基金资助:
    国家自然科学基金资助项目(31660468);校企合作科研项目(XZ20190612)

Abstract:

To investigate the bacterial diversity of the traditional fermented yak milk in the Gannan area of Gansu province, the V3-V4 regions of 16S rRNA from 13 fermented yak milks were sequenced using Illumina MiSeq high-throughput sequencing technology to analyze the bacterial flora diversity of traditional fermented yak milks in Gannan, Gansu Province. The results showed that 720 221 high-quality 16S rRNA gene sequences were generated and four different bacterial phyla and four different bacterial genera were identified. Firmicutes was the dominant phylum, Lactobacillus was the high abundance genus, and yak yogurt from different sources showed different abundance at the phylum level. PICRUSt analysis based on 16S rRNA sequencing data of each sample revealed that the basic predicted function accounted for 11.76% of all genes. This study can build a basis for the protection and utilization of microbial resources in the traditional fermented yak milk in Gannan, Gansu.

Key words: traditional fermented yak, high-throughput sequencing, bacterial diversity

摘要:

为了研究甘肃甘南传统发酵牦牛乳中细菌菌群的多样性,本试验以甘肃甘南地区传统发酵牦牛乳为研究对象,基于Illumina MiSeq高通量测序技术,对13份发酵牦牛乳的16S rRNA中V3-V4区进行测序,分析甘肃甘南地区传统发酵牦牛乳中细菌菌群的多样性。结果表明,最终获得720 221条高质量16S rRNA 基因序列,共鉴定出4种不同细菌门、4种不同细菌属,其中厚壁菌门(Firmicutes)为优势菌门,乳杆菌属(Lactobacillus)为高丰度菌属,不同来源的发酵牦牛乳在门属水平上呈现不同的丰度。基于每个样品的16S rRNA测序数据进行PICRUSt功能预测,分析发现,基本预测功能占主导地位,占所有基因的11.76%。本研究结果为今后甘肃甘南传统发酵牦牛乳中微生物资源的保护和利用提供了理论依据。

关键词: 传统发酵牦牛酸乳, 高通量测序, 细菌多样性