Journal of Nuclear Agricultural Sciences ›› 2022, Vol. 36 ›› Issue (1): 94-104.DOI: 10.11869/j.issn.100-8551.2022.01.0094

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles     Next Articles

Proteomic Analysis Reveals Mechanisms of Stropharia rugosoannulata Polysaccharides Promoting Kiwifruit Growth and High Temperature Resistance

XIAO Wenfei(), CHAI Weiguo, QIU Jieren, XIN Ya, RUAN Songlin*()   

  1. Hangzhou Academy of Agricultural Sciences, Hangzhou, Zhejiang 310024
  • Received:2020-08-07 Accepted:2020-10-04 Online:2022-01-10 Published:2021-12-06
  • Contact: RUAN Songlin


肖文斐(), 柴伟国, 裘劼人, 忻雅, 阮松林*()   

  1. 杭州市农业科学研究院, 浙江 杭州 310024
  • 通讯作者: 阮松林
  • 作者简介:肖文斐, 女, 高级农艺师, 主要从事植物生物技术研究。E-mail:
  • 基金资助:


In order to assess the effect of Stropharia rugosoannulata polysaccharides (SPS) on the growth and induced resistance of kiwifruit and unravel the regulation mechanism, leaf morphology and structure, physiological and proteomic analysis were carried out with the variety Hongyang. Our results showed that compared with the control, the area, thickness and chlorophyll content of its leaves increased after treatment with SPS. The cross section of blade showed that the palisade tissue and spongy tissue were closely arranged and thickened after being sprayed with SPS. Under high temperature stress, the chlorophyll fluorescence Fv/Fm ratio, peroxidase and polyphenoloxidase activity of the treated leaves increased significantly. A total of 326 differentially accumulated proteins (DAPs) were identified by label-free quantitative proteomics, including 166 up-regulated proteins (up-regulated by 1.5-fold and P<0.05) and 160 down-regulated proteins (down-regulated by 1.5-fold and P <0.05). Gene ontology annotation revealed that SPS dramatically changed the levels of proteins related to chloroplast, stress response and redox and the pathways involved in photosynthesis, hormone signaling and biosynthesis of secondary metabolites. S. rugosoannulata polysaccharides may improve photosynthetic efficiency and promote the growth of kiwifruit leaves via regulating the expression of proteins related to photosynthesis. The regulation on stress response and redox related proteins indicated that SPS may have a potential role in inducing system resistance of kiwifruit. Our results provide new resources and a theoretical basis for the development and utilization of polysaccharide resistance inducers.

Key words: kiwifruit, Stropharia rugosoannulata polysaccharides, promoting growth, high temperature stress, proteomics



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