Correlation Between the Accumulations of 9 Steviol Glycosides and the Expressions of the Key Genes Involved in Their Biosynthesis in Stevia rebaudiana

doi:10.11869/j.issn.100-8551.2022.01.0075

Journal of Nuclear Agricultural Sciences ›› 2022, Vol. 36 ›› Issue (1): 75-82.DOI: 10.11869/j.issn.100-8551.2022.01.0075

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles Next Articles

Correlation Between the Accumulations of 9 Steviol Glycosides and the Expressions of the Key Genes Involved in Their Biosynthesis in Stevia rebaudiana

ZHANG Hong(), LU Guodong, YUAN Chunchun, LANG Sirui, CHEN Ren^*()

School of Life Science, NingxiaUniversity/Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China/Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia, Yinchuan, Ningxia 750021

Received:2020-10-29 Accepted:2021-01-07 Online:2022-01-10 Published:2021-12-06
Contact: CHEN Ren

甜叶菊中9种甜菊醇糖苷积累与其生物合成关键基因表达量的相关性

张虹(), 路国栋, 袁春春, 郎思睿, 陈任^*()

宁夏大学生命科学学院/西部特色生物资源保护与利用教育部重点实验室/宁夏优势特色作物现代分子育种重点实验室, 宁夏银川 750021

通讯作者: 陈任
作者简介:张虹,女,副教授,主要从事植物基因工程研究。E-mail: zhhong1225@aliyun.com
基金资助:
国家自然科学基金资助项目(31860065);宁夏自然科学基金项目(2020AAC03106)

Abstract

Abstract:

In order to study the correlation between the accumulations of main steviol glycosides (SGs) and the expressions of the key genes that are involved in their biosynthesis in Stevia rebaudiana, three cultivars (Pusaike3, Zhongshan2, and Tongxin) were selected as materials. The upper three layer leaves were harvested at 3 growth stages, including the young seedling stage, vegetative stage, and flower budding stage. The contents of 9 SGs, such as rebaudioside A, B, C, D, E, F (RA, RB, RC, RD, RE, RF), stevioside (ST), rubusoside (RBS), dulcoside A (DA), were test by liquid chromatography mass spectrometry (LC-MS), and the expression levels of 3 key genes encoding uridine diphosphate-glycosyltransferases (UGTs), SrUGT91D2e, SrUGT74G1 and SrUGT76G1, were analyzed by quantitative real-time PCR. The results showed that the contents of the sweet SGs, RA was the highest at vegetative stage (1.60 mg·g^-1), and RD was the highest at flower budding stage (1.58 mg·g^-1) in Pusaike3; the contents of bitter SGs, St (0.50 mg·g^-1), RC (0.26 mg·g^-1) and DA (0.01 mg·g^-1) were significant lower in Pusaike3 than those in Zhongshan 2 or Tongxin, thus Pusaike3 was considered as a candidate cultivar for high yield of sweet SGs. The correlation analysis between the contents of SGs and the expressions levels of the key genes involved in their biosynthesis showed that the contents of sweet SGs, RD and RA were remarkably correlated with the expression levels of SrUGT91D2e and SrUGT76G1, while the contents of bitter SGs, RC, ST, DA were remarkably correlated with the expression level of SrUGT74G1. This research provides a scientific basis for Stevia rebaudiana breeding and suitable harvest time selection.

Key words: Stevia rebaudiana, steviol glycosides, gene expression, correlation analysis

摘要：

为了研究甜叶菊中主要甜菊醇糖苷积累特点与其生物合成关键基因表达量的关系,本研究选取3个品种甜叶菊(普赛科3号、中山2号、同心)为试验材料,定期在幼苗期、营养生长期、花蕾期采收植株的上位3对叶片,采用液相色谱-质谱法(LC-MS)检测9种甜菊醇糖苷,即莱宝迪苷A、B、C、D、E、F(RA、RB、RC、RD、RE、RF)、甜菊糖苷(ST)、甜茶糖苷(RBS)和杜尔可苷A(DA)含量,同时利用实时荧光定量PCR(qRT-PCR)分析糖苷生物合成的3个UDP-糖基转移酶(UGTs)关键基因,即SrUGT91D2e、SrUGT74G1和SrUGT76G1的表达水平。结果表明,甜味糖苷RA在普塞科3号营养生长期含量最高(1.60 mg·g^-1), RD在普塞科3号的花蕾期含量最高(1.58 mg·g^-1);苦味糖苷ST、RC、DA在普塞科3号中的积累量显著低于中山2号和同心(普塞科3号ST为0.50 mg·g^-1、RC为0.26 mg·g^-1、DA为0.01 mg·g^-1),因此普塞科3号可作为甜味糖苷高产选育品种。对甜菊醇糖苷生物合成关键基因表达量与糖苷含量进行相关性分析,甜味糖苷RD和RA的含量与其关键影响因子SrUGT91D2e和SrUGT76G1的表达量显著相关,苦味糖苷RC、ST、DA的含量与其关键影响因子SrUGT74G1的表达量显著相关。本试验为甜叶菊不同甜度糖苷的品种选育及采收时间的确定提供了依据。

关键词: 甜叶菊, 甜菊醇糖苷, 基因表达, 相关性分析

ZHANG Hong, LU Guodong, YUAN Chunchun, LANG Sirui, CHEN Ren. Correlation Between the Accumulations of 9 Steviol Glycosides and the Expressions of the Key Genes Involved in Their Biosynthesis in Stevia rebaudiana[J]. Journal of Nuclear Agricultural Sciences, 2022, 36(1): 75-82.

张虹, 路国栋, 袁春春, 郎思睿, 陈任. 甜叶菊中9种甜菊醇糖苷积累与其生物合成关键基因表达量的相关性[J]. 核农学报, 2022, 36(1): 75-82.

Figures/Tables 6

References 28

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创刊：1987年
主管部门：农业农村部
主办单位：中国原子能农学会与中国农业科学院农产品加工研究所（前中国农业科学院原子能利用研究所）
ISSN：1000-8551

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基因名称 Gene name	引物序列(5'-3') Primer sequence (5'-3')	扩增长度 Amplication length/bp
SrGAPDH	CGGTGTCAATGAGCACGAAT	58
	TGCAGCTAGCGTTTGAAACAA
SrUGT76G1	TGGTCCGCTCGCTGGTAT	55
	TCGTCAGCTCCGTGTTCGT
SrUGT74G1	GAGCAAATACAGAGCCCTTGGT	69
	CCAACGTGCTTGATCAATATTAGC
SrUGT91D2e	ACGGACGTCCACACTCATGA	59
	GGCTGAAGTCCGTCAAAAGC

基因名称 Gene name	引物序列(5'-3') Primer sequence (5'-3')	扩增长度 Amplication length/bp
SrGAPDH	CGGTGTCAATGAGCACGAAT	58
	TGCAGCTAGCGTTTGAAACAA
SrUGT76G1	TGGTCCGCTCGCTGGTAT	55
	TCGTCAGCTCCGTGTTCGT
SrUGT74G1	GAGCAAATACAGAGCCCTTGGT	69
	CCAACGTGCTTGATCAATATTAGC
SrUGT91D2e	ACGGACGTCCACACTCATGA	59
	GGCTGAAGTCCGTCAAAAGC

品种 Cultivar	时期 Stage	甜菊醇糖苷含量SGs content/(mg·g^-1)
品种 Cultivar	时期 Stage	RA	RB	RC	RD	RE	RF	ST	RBS	DA
Pu3	S1	1.44±0.10b	0.16±0.01a	0.22±0.05b	1.13±0.03c	0.03±0.01a	0.02±0.00a	0.54±0.03a	0.04±0.02a	0.01±0.00a
	S2	1.60±0.04a	0.25±0.00a	0.41±0.02a	1.41±0.04b	0.05±0.00a	0.04±0.00a	0.43±0.02a	0.02±0.00a	0.01±0.00a
	S3	1.27±0.12c	0.22±0.02a	0.27±0.05b	1.58±0.04a	0.11±0.01a	0.02±0.01a	0.50±0.02a	0.01±0.00a	0.01±0.00a
ZS2	S1	1.17±0.04a	0.12±0.05b	0.49±0.07b	0.54±0.05c	0.11±0.01b	0.28±0.01c	0.93±0.03a	0.09±0.04a	0.40±0.03a
	S2	1.26±0.14a	0.24±0.08a	0.68±0.00a	0.79±0.00b	0.14±0.09b	0.49±0.00b	0.77±0.05b	0.04±0.01b	0.26±0.00b
	S3	1.23±0.10a	0.23±0.05a	0.70±0.05a	0.85±0.05a	0.29±0.01a	0.79±0.07a	0.91±0.07a	0.15±0.03a	0.41±0.02a
TX	S1	1.20±0.06a	0.08±0.02b	0.51±0.04b	0.38±0.05b	0.14±0.01b	0.43±0.03c	0.98±0.03a	0.16±0.05a	0.49±0.09b
	S2	1.14±0.02a	0.17±0.03a	0.71±0.02a	0.70±0.11a	0.50±0.01a	1.25±0.06a	1.05±0.02a	0.01±0.01b	0.61±0.02a
	S3	1.08±0.03a	0.19±0.03a	0.59±0.00b	0.68±0.08a	0.46±0.04a	0.96±0.06b	1.02±0.01a	0.21±0.00a	0.63±0.02a

品种 Cultivar	时期 Stage	甜菊醇糖苷含量SGs content/(mg·g^-1)
品种 Cultivar	时期 Stage	RA	RB	RC	RD	RE	RF	ST	RBS	DA
Pu3	S1	1.44±0.10b	0.16±0.01a	0.22±0.05b	1.13±0.03c	0.03±0.01a	0.02±0.00a	0.54±0.03a	0.04±0.02a	0.01±0.00a
	S2	1.60±0.04a	0.25±0.00a	0.41±0.02a	1.41±0.04b	0.05±0.00a	0.04±0.00a	0.43±0.02a	0.02±0.00a	0.01±0.00a
	S3	1.27±0.12c	0.22±0.02a	0.27±0.05b	1.58±0.04a	0.11±0.01a	0.02±0.01a	0.50±0.02a	0.01±0.00a	0.01±0.00a
ZS2	S1	1.17±0.04a	0.12±0.05b	0.49±0.07b	0.54±0.05c	0.11±0.01b	0.28±0.01c	0.93±0.03a	0.09±0.04a	0.40±0.03a
	S2	1.26±0.14a	0.24±0.08a	0.68±0.00a	0.79±0.00b	0.14±0.09b	0.49±0.00b	0.77±0.05b	0.04±0.01b	0.26±0.00b
	S3	1.23±0.10a	0.23±0.05a	0.70±0.05a	0.85±0.05a	0.29±0.01a	0.79±0.07a	0.91±0.07a	0.15±0.03a	0.41±0.02a
TX	S1	1.20±0.06a	0.08±0.02b	0.51±0.04b	0.38±0.05b	0.14±0.01b	0.43±0.03c	0.98±0.03a	0.16±0.05a	0.49±0.09b
	S2	1.14±0.02a	0.17±0.03a	0.71±0.02a	0.70±0.11a	0.50±0.01a	1.25±0.06a	1.05±0.02a	0.01±0.01b	0.61±0.02a
	S3	1.08±0.03a	0.19±0.03a	0.59±0.00b	0.68±0.08a	0.46±0.04a	0.96±0.06b	1.02±0.01a	0.21±0.00a	0.63±0.02a

基因名称 Gene name	相关系数/Correlation coefficient
基因名称 Gene name	RA	RB	RC	RD	RE	RF	ST	RBS	DA
SrUGT76G1	0.797^*	0.528	-0.223	0.621	-0.279	-0.289	-0.667^*	-0.602	-0.574
SrUGT91D2e	0.225	0.570	-0.485	0.812^**	-0.144	-0.366	-0.571	-0.692^*	-0.508
SrUGT74G1	-0.767^*	-0.248	0.800^**	-0.763^*	0.714^*	0.808^**	0.906^**	0.529	0.885^**

Correlation Between the Accumulations of 9 Steviol Glycosides and the Expressions of the Key Genes Involved in Their Biosynthesis in Stevia rebaudiana

甜叶菊中9种甜菊醇糖苷积累与其生物合成关键基因表达量的相关性

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