Journal of Nuclear Agricultural Sciences ›› 2022, Vol. 36 ›› Issue (1): 1-6.DOI: 10.11869/j.issn.100-8551.2022.01.0001

• Induced Mutations for Plant Breeding·Agricultural Biotechnology •     Next Articles

Production and Insect-Resistant Characterization of Hanhui 3T a Derivative Line of Huahui No.1

YE Shuifeng1(), LOU Jue2,*(), GAO Ningning3, YANG Xin1, DENG Jielou1   

  1. 1College of Life Sciences, Shangrao Normal University, Shangrao, Jiangxi 334001
    2Southern Zhejiang Key Laboratory of Crop Breeding,Wenzhou Vocational College of Science and Technology/Wenzhou Academy of Agricultural Sciences, Wenzhou, Zhejiang 325006
    3Shanghai Agrobiological Gene Center, Shanghai 201106
  • Received:2020-11-23 Accepted:2021-01-07 Online:2022-01-10 Published:2021-12-06
  • Contact: LOU Jue


叶水烽1(), 楼珏2,*(), 高宁宁3, 杨锌1, 邓接楼1   

  1. 1上饶师范学院生命科学学院,江西 上饶 334001
    2温州科技职业学院/温州市农业科学研究院 浙南作物育种重点实验室,浙江 温州 325006
    3上海市农业生物基因中心,上海 201106
  • 通讯作者: 楼珏
  • 作者简介:叶水烽,男,副教授,从事植物种质创新及功能基因研究。E-mail:
  • 基金资助:


As one of the major food crops of the world, rice is susceptible to major pests such as stem borers, rice planthoppers, rice weevil, that cause the reduction of yield. In order to improve the insect resistance of water-saving and drought resistant rice, Huahui No.1 (TT51) was used as Cry1Ab/Ac gene donor to cross with Hanhui No.3, a water-saving and drought-resistance rice restorer line. The target gene was screened by molecular marker assisted selection (MAS) technology, and BC3F4 homozygous and stable lines were obtained. Through qualitative and quantitative detection of Cry1Ab/Ac protein, combined with field agronomic traits and insect resistance observation, a new insect resistant restorer line Hanhui 3T was selected. The results showed that compared with the control Hanhui No.3, Hanhui 3T had similar agronomic traits, but obvious resistance to Cnaphalocrocis medinalis. The hybrid plants of Huhan 5A/Hanhui 3T were also highly resistant to Cnaphalocrocis medinalis, while the control hybrid plants of Huhan 5A/Hanhui No.3 were damaged by Cnaphalocrocis medinalis. The results of this study provide a material foundation for breeding new insect resistance rice varieties with water saving and drought resistance.

Key words: insect-resistance, Cry1Ab/Ac protein, water-saving and drought-resistance rice, hybrids



关键词: 抗虫, Cry1Ab/Ac蛋白, 节水抗旱稻, 杂交