Journal of Nuclear Agricultural Sciences ›› 2022, Vol. 36 ›› Issue (1): 1-6.DOI: 10.11869/j.issn.100-8551.2022.01.0001

• Induced Mutations for Plant Breeding·Agricultural Biotechnology •     Next Articles

Production and Insect-Resistant Characterization of Hanhui 3T a Derivative Line of Huahui No.1

YE Shuifeng1(), LOU Jue2,*(), GAO Ningning3, YANG Xin1, DENG Jielou1   

  1. 1College of Life Sciences, Shangrao Normal University, Shangrao, Jiangxi 334001
    2Southern Zhejiang Key Laboratory of Crop Breeding,Wenzhou Vocational College of Science and Technology/Wenzhou Academy of Agricultural Sciences, Wenzhou, Zhejiang 325006
    3Shanghai Agrobiological Gene Center, Shanghai 201106
  • Received:2020-11-23 Accepted:2021-01-07 Online:2022-01-10 Published:2021-12-06
  • Contact: LOU Jue

华恢1号衍生系旱恢3T的选育及抗虫表现

叶水烽1(), 楼珏2,*(), 高宁宁3, 杨锌1, 邓接楼1   

  1. 1上饶师范学院生命科学学院,江西 上饶 334001
    2温州科技职业学院/温州市农业科学研究院 浙南作物育种重点实验室,浙江 温州 325006
    3上海市农业生物基因中心,上海 201106
  • 通讯作者: 楼珏
  • 作者简介:叶水烽,男,副教授,从事植物种质创新及功能基因研究。E-mail: shuifengye@sru.edu.cn
  • 基金资助:
    转基因生物新品种培育重大专项(2016ZX08001001-001);上海市自然科学基金(18ZR1433400);浙南作物育种重点实验室开放课题研究基金(2020SZCB02);上饶师范学院博士科研启动项目(K6000330)

Abstract:

As one of the major food crops of the world, rice is susceptible to major pests such as stem borers, rice planthoppers, rice weevil, that cause the reduction of yield. In order to improve the insect resistance of water-saving and drought resistant rice, Huahui No.1 (TT51) was used as Cry1Ab/Ac gene donor to cross with Hanhui No.3, a water-saving and drought-resistance rice restorer line. The target gene was screened by molecular marker assisted selection (MAS) technology, and BC3F4 homozygous and stable lines were obtained. Through qualitative and quantitative detection of Cry1Ab/Ac protein, combined with field agronomic traits and insect resistance observation, a new insect resistant restorer line Hanhui 3T was selected. The results showed that compared with the control Hanhui No.3, Hanhui 3T had similar agronomic traits, but obvious resistance to Cnaphalocrocis medinalis. The hybrid plants of Huhan 5A/Hanhui 3T were also highly resistant to Cnaphalocrocis medinalis, while the control hybrid plants of Huhan 5A/Hanhui No.3 were damaged by Cnaphalocrocis medinalis. The results of this study provide a material foundation for breeding new insect resistance rice varieties with water saving and drought resistance.

Key words: insect-resistance, Cry1Ab/Ac protein, water-saving and drought-resistance rice, hybrids

摘要:

水稻作为世界上主要的粮食作物之一,容易受到螟虫、稻飞虱、稻象甲等主要害虫为害造成减产。为改良节水抗旱稻的抗虫性,以华恢1号(TT51)为cry1Ab/Ac基因供体,与节水抗旱稻恢复系品种旱恢3号杂交,利用分子标记辅助选择技术对目标基因进行筛选,最终获得BC3F4纯合、稳定株系。通过对Cry1Ab/Ac蛋白的定性和定量检测,结合田间农艺性状及抗性观察,筛选出抗虫新恢复系旱恢3T。结果显示,与对照旱恢3号相比,旱恢3T的农艺性状无显著差异,但对稻纵卷叶螟具有明显抗性。与不育系沪旱5A配组的杂交稻组合沪旱5A/旱恢3T也高抗稻纵卷叶螟,采用对照旱恢3号恢复系与沪旱5A配组的组合沪旱5A/旱恢3号则受稻纵卷叶螟为害。本研究结果为培育既节水抗旱又抗虫的水稻新品种奠定了材料基础。

关键词: 抗虫, Cry1Ab/Ac蛋白, 节水抗旱稻, 杂交