Journal of Nuclear Agricultural Sciences ›› 2020, Vol. 34 ›› Issue (8): 1621-1630.DOI: 10.11869/j.issn.100-8551.2020.08.1621

• Induced Mutations for Plant Breeding·Agricultural Biotechnology •     Next Articles

Effects of 137Cs-γ Rays Irradiation on Germination and Seedling Growth of Clematis chinensis

YE Ying, LIU Zhigao*, LIANG Lijun, JI Mengcheng   

  1. School of Landscape Architecture, Zhejiang A&F University, Hangzhou, Zhejiang 311300
  • Received:2019-08-26 Revised:2019-11-12 Online:2020-08-10 Published:2020-06-16

137Cs-γ射线辐射对威灵仙种子发芽及幼苗生长的影响

叶盈, 刘志高*, 梁立军, 季梦成   

  1. 浙江农林大学风景园林与建筑学院,浙江 杭州 311300
  • 通讯作者: *刘志高,男,副教授,主要从事园林植物栽培与应用研究。E-mail:vzhigao@zafu.edu.cn
  • 作者简介:叶盈,女,主要从事园林植物遗传育种与种质创新研究。E-mail:hz_yy94@foxmail.com
  • 基金资助:
    浙江省花卉新品种选育重大科技专项重点项目(2012C12909-16),浙江省重点研发计划项目(2018C02G2100757)

Abstract: In order to cultivate Clematis chinensis species, the seeds of Clematis chinensis were used as experimental materials to conduct radiation treatment with different dose (0, 50, 100, 150, 200 Gy) of 137Cs-γ rays the influence of radiation on seed germination and seedling growth of Clematis chinensis were studied. The results showed that 50 Gy could promote seed germination and seedling formation. The LD50 of Clematis chinensis was found to be 109.5 Gy. After radiation treatment, 7 kinds of mutation in leaf shapes were appeared including the heart shape, among which 50 and 100 Gy treatment induced the most mutation, with five and four types respectively. Seedling height, tiller number, ground diameter and internode length decreased with the increase of radiation doses. With the increase of radiation doses, relative chlorophyll content decreased, Fv/Fm, Fv/Fo and ETR overall increased first and then decreased, reaching the maximum value at 50 Gy. Higher radiation doses inhibited the activity of the photosynthetic system. qPincreased with the increase of radiation doses. Both of ETR and NPQ showed a downward trend with the increase of radiation doses, dectreased to the lowest at the treatment of 100 Gy. Experiments showed that, the seedlings irradiated by 50-100 Gy rays have rich variations in morphology, proportion of variation, high quality growth and well-performed photosynthetic system. Considering LD50of Clematis chinensis, the appropriate doses range of 137Cs-γ irradiation for Clematis chinensis breeding is 50-109.5Gy. The result provided a scientific basis for radiation breeding in Clematis. L.

Key words: Clematis chinensis, 137Cs-γ radiation, seed germination, seedling morphology, chlorophyⅡ fluorescence

摘要: 为培育威灵仙(Clematis chinensis)品种,以威灵仙种子为试验材料,用不同剂量(0、50、100、150、200 Gy)的137Cs-γ射线进行辐射处理,研究辐射对威灵仙种子发芽及幼苗生长的影响。结果表明,50 Gy辐射剂量可促进种子发芽和成苗;威灵仙种子辐射半致死剂量(LD50)为109.5 Gy。辐射处理后植株的叶形呈现心形等7种变异,其中50和100 Gy辐射剂量的变异叶形种类较多,分别出现了5种和4种变异。植株苗高、分蘖数、地径和节间长度随着辐射剂量的增加而降低。此外,随着辐射剂量的增加,相对叶绿素含量降低; Fv/FmFv/Fo和ETR总体呈先升后降的趋势,均在50 Gy剂量下达到最大值;qP呈增加趋势;NPQ均呈下降趋势,100 Gy剂量时,两者降低最显著。试验表明辐射剂量为50~100 Gy时,威灵仙幼苗茎、叶变异比例较高,植株生长和光合系统运转状况良好。结合威灵仙种子半致死剂量认为,137Cs-γ辐射威灵仙育种的适宜剂量范围是50~109.5 Gy。本文研究结果为铁线莲辐射育种提供了科学依据。

关键词: 威灵仙, 137Cs-γ辐射, 种子萌发, 幼苗形态, 叶绿素荧光