Journal of Nuclear Agricultural Sciences ›› 2020, Vol. 34 ›› Issue (6): 1163-1177.DOI: 10.11869/j.issn.100-8551.2020.06.1163

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles     Next Articles

Transcriptome Sequencing and Analysis of Functional Genes in Young Leaves of Bottle Gourd

XU Duanxiang*, ZHAO Ruili, CHEN Zhongshan, DU Wenli, XU Tongwei, GAO Shan*   

  1. Fuzhou Institute of Vegetable Science, Fuzhou, Fujian 350111
  • Received:2019-03-26 Revised:2019-06-09 Online:2020-06-10 Published:2020-04-19

瓠瓜幼叶转录组功能基因测序及分析

许端祥*, 赵瑞丽, 陈中钐, 杜文丽, 徐同伟, 高山*   

  1. 福州市蔬菜科学研究所,福建 福州 350111
  • 通讯作者: 许端祥,男,副研究员,主要从事蔬菜育种及生物技术研究。E-mail:371055736@qq.com;
    高山,男,研究员,主要从事蔬菜育种及生物技术研究。E-mail: vegao@163.com。同为通讯作者。
  • 作者简介:许端祥,男,副研究员,主要从事蔬菜育种及生物技术研究。E-mail:371055736@qq.com
  • 基金资助:
    福建省星火项目(2018S0004),福建省农业科技重大专项专题项目(2018NZ0002-3)

Abstract: In order to explore the functional gene information of transcriptome in bottle gourd, the transcriptome of Fuzhou bottle gourd was sequenced by high-throughput non-parametric sequencing technology and 664 252 268 reads fragments were obtained. The reads gave 87 518 Unigenes with the total length of 91 405 320 bp, and 76.03% of Unigenes were concentrated in 1-1 000 bp. The results of functional annotations showed that 55 725 Unigenes were annotated in Nr database, among which 26 354 Unigenes were annotated in cucurbitaceae, accounting for 47.29% of the total Unigenes. GO database annotated 18 278 Unigenes, which closely related to molecular function, cell structure function and biological process function with 55 subgroups. 41 635 Unigenes were annotated in COG database, which were divided into information storage and processing, cell process and signal transmission, metabolism and non-characteristic genes with a total of 25 functional groups and the number of genes involved in amino acid transport and metabolism was the largest. There are 24 770 Unigenes annotated in the KEGG database, involving 220 KEGG metabolic pathways. SSR locus retrieval results showed that there were 11 029 SSR loci in 8 617 Unigenes containing SSR sequences, with a frequency of 9.846%. The results of this study would lay a foundation for the further development of metabolic pathways and SSR molecular markers in bottle gourd.

Key words: bottle gourd, transcriptome, functional analysis, SSR

摘要: 为探讨瓠瓜转录组功能基因信息,对福州芋瓠瓠瓜叶片进行了Illumina Hiseqxten高通量无参转录组测序分析,共获得664 252 268个reads片段,经序列组装共计获得87 518个Unigene,总长度高达91 405 320 bp,76.03%的Unigene长度主要集中在1~1 000 bp。功能注释结果显示,有55 725个Unigene在Nr数据库获得注释,注释到葫芦科的Unigene数量最多,有26 354个,占总Unigene的47.29%。GO数据库注释到18 278个Unigene,可以分为分子功能、细胞组分和生物学过程共三大类的55个亚类。COG数据库中有41 635个Unigene获得注释,分布在信息存储与处理、细胞过程和信号传递、新陈代谢、无特征基因四大类的25个功能区域,其中参与氨基酸运输和代谢的Unigene数量最多。KEGG数据库中有24 770个Unigene获得注释,涉及到220个KEGG代谢途径。SSR位点检索结果显示,包含SSR序列的8 617条Unigene中存在11 029个SSR位点,发生频率为9.846%。本研究结果为进一步深入开展瓠瓜功能基因研究和SSR分子标记开发奠定了重要基础。

关键词: 瓠瓜, 转录组, 功能分析, SSR