Journal of Nuclear Agricultural Sciences ›› 2020, Vol. 34 ›› Issue (3): 669-679.DOI: 10.11869/j.issn.100-8551.2020.03.0669

• Isotope Tracer Technique·Ecology & Environment·Physiology • Previous Articles    

Establishment of Evaluation System for Antioxidant Index of Heat Tolerance of Green Stalks of Non-heading Chinese Cabbage

ZOU Kaixi1, YANG Yan2, TIAN Libo1,*, SHANG Sang1, ZENG Liping1, GUO Xuesong1, LIU Ziji2, ZHU Guopeng1   

  1. 1 College of Horticulture, Hainan University/The Key Laboratory of Tropical Horticultural Crop Quality Reyulation of Hannan Province,Haikou, Hainan 570228;
    2 Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs,Danzhou, Hainan 571737
  • Received:2018-09-12 Online:2020-03-10 Published:2020-01-20

青梗不结球白菜耐热性的抗氧化指标评估体系建立

邹凯茜1, 杨衍2, 田丽波1,*, 商桑1, 曾丽萍1, 郭雪松1, 刘子记2, 朱国鹏1   

  1. 1 海南大学园艺学院/海南省热带园艺作物品质调控重点实验室,海南 海口 570228;
    2 中国热带农业科学院热带作物品种资源研究所/农业农村部华南作物基因资源与种质创制重点开放实验室,海南 儋州 571737
  • 通讯作者: 田丽波,女,副教授,主要从事蔬菜遗传育种研究。E-mail: faiy7play@163.com
  • 作者简介:邹凯茜,女,主要从事园艺植物抗逆性研究。E-mail:1046615125@qq.com
  • 基金资助:
    2017年海南省重大科技计划项目(ZDKJ2017001),农业农村部财政专项项目(NFZX2018),2018年海南省研究生创新科研课题(Hys2018-34)

Abstract: In order to explore the germplasm resources and recommend excellent variety of heat-tolerant non-heading Chinese cabbage for production, this study applied multivariate statistical method to comprehensively analyze the antioxidant related indexes of 19 green stalks of non-heading Chinese cabbage with high temperature stress treated. Results showed that after 5 days of high temperature stress, the average coefficient of variation of each index for different cultivar was 33.26%. The rate of superoxide anion radical generation is significantly different among varieties, and the other indicators are significantly different. The correlations of these indexes reached significant level and six of them are most significant; the rate of superoxide anion radical (O2·-) production was significant and positively correlated with the content of hydrogen peroxide (H2O2). Thirteen indicators were converted into 5 domain components which explained 74.5% of the source of difference. According to the principal component score, the membership function was calculated to obtain the comprehensive index D value, and the tested materials were ranked. The top three heat-tolerance varieties were Qing 16, Qing 20 and Qing 2, and the last three were Qing 1 and Qing 8 and Qing 15. According to the D value, 19 kinds of green stalks non-heading Chinese cabbage were clustered into three clusters. In the comprehensive evaluation of clustering results, APX, AsA, MDA, POD heat-tolerance cofficience, and HII are inversely proportional to heat-tolerance. The comprehensive evaluation model D=0.752 75-0.021 90APX-0.042 48MDA-0.034 08AsA-0.079 84POD-0.22561HII is obtained by multiple regression analysis which can be used as a comprehensive evaluation model for heat tolerance of green stalks non-heading Chinese cabbage. These results provided a scientific and accurate evaluation system for heat tolerance resources exploitation and cultivars selected breeding of non-heading Chinese cabbage.

Key words: non-heading Chinese cabbage, green stalk, principal component analysis, membership function, heat-tolerance

摘要: 为发掘耐热性不结球白菜种质资源并为生产推荐耐热性优良品种,本试验采用多元统计的方法对高温胁迫处理的19个青梗不结球白菜品种的幼苗抗氧化相关指标进行综合分析。结果表明,高温胁迫5 d后,不同品种各指标的平均变异系数为33.26%,品种间除超氧阴离子自由基(O2·-)产生速率差异显著,其他指标均差异极显著。各指标相关性达显著、极显著的有6个,其中O2·-产生速率与过氧化氢(H2O2)含量呈极显著正相关。将13个指标降维成5个主成分,解释了74.5%的差异来源。根据主成分得分计算隶属函数获得综合指标D值,对供试材料进行排序,筛选出耐热性前三位的品种分别为青16、青20、青2,后三位品种为青1、青8、青15。并根据D值将19种青梗不结球白菜聚为三类,聚类结果综合评价中APX、AsA、MDA、POD耐热系数和HII均与耐热性成反比,将各指标进行多元回归分析,获得精度高的综合评价模型D=0.752 75-0.021 90APX-0.042 48MDA-0.034 08AsA-0.079 84POD -0.225 61HII,可作为青梗不结球白菜耐热性综合评价模型。本研究结果为不结球白菜耐热性和品种选育的科学准确的评价体系的建立提供了一定的理论基础。

关键词: 不结球白菜, 青梗, 主成分分析, 隶属函数, 耐热性