Journal of Nuclear Agricultural Sciences ›› 2020, Vol. 34 ›› Issue (2): 281-287.DOI: 10.11869/j.issn.100-8551.2020.02.0281

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles     Next Articles

Expression Characteristics of miR-29a in Different Tissues of Youzhou Dark Goats and Its Dynamic Expression in B16 Cells

CHEN Cancan1,2, LI Jie2, FU Lin2, SUN Xiaoyan2, WANG Gaofu2, ZHOU Peng2, MA Youji1,*, REN Hangxing2,*   

  1. 1College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070;
    2Chongqing Academy of Animal Science, Chongqing 402460
  • Received:2018-08-23 Online:2020-02-10 Published:2019-12-13

miR-29a在酉州乌羊不同体组织中表达特征及其在B16细胞中的动态表达分析

陈灿灿1,2, 李杰2, 付琳2, 孙晓燕2, 王高富2, 周鹏2, 马友记1,*, 任航行2,*   

  1. 1甘肃农业大学动物科学技术学院,甘肃 兰州 730070;
    2重庆市畜牧科学院,重庆 402460
  • 通讯作者: 马友记,男,教授,主要从事绵山羊繁育与疫病防治研究。E-mail: yjma@gsau.edu.cn;任航行,男,副研究员,主要从事羊遗传育种研究。E-mail: rhxe@163.com。同为通讯作者。
  • 作者简介:陈灿灿,男,主要从事动物遗传育种与繁殖研究。E-mail:491892115@qq.com
  • 基金资助:
    国家自然科学基金项目(31672393),重庆市社会民生类重点研发项目(cstc2017-zdyfX0045)

Abstract: To investigate the expression characteristics of miR-29a in different tissues of Youzhou dark goat and its expression level in B16 cells. In this study, RT- qPCR was used to detect the expression level of miR-29a in different tissues of Youzhou dark goat and B16 cells at different developmental stages. It showed that miR-29a expressed in different tissues of Youzhou dark goats,with the highest relative expression level in the brain and the lowest level in lung and skin. The relative expression of miRNA-29a in the skin of Yudong white goat was 3.44 times(P<0.01) the expression in the skin of dark goat and displayed significant differences between them. In general, the expression of miRNA-29a increased significantly (P<0.01) in the process of cell proliferation, and significantly increased in the early differential period then become stable with non-significance (P>0.05). Furthermore,the cell morphology was changed and melanin secretion was increased after adding the differentiation medium. In conclusion, miR-29a is expressed widely in different tissues of Youzhou dark goat. The relative expression of miR-29a increased with the proliferation of B16 cells, but decreased with the differentiation of melanocytes,which suggested that miR-29a was closely related to the proliferation and differentiation of B16 cells.The results provided some theoretical evidences for clarifying the expression characteristics of miRNA-29a in Youzhou dark goat tissues and its’ involvement involved in the regulation of melanocyte behavior.

Key words: miR-29a, Youzhou dark goat, B16 cell, expression characteristics, morphology

摘要: 为探究miR-29a在酉州乌羊不同体组织表达特征及在B16细胞中的表达特性,本研究采用RT-qPCR方法检测miR-29a在酉州乌羊不同体组织以及B16细胞不同发育时期的表达水平。结果表明,miR-29a在酉州乌羊各体组织均有表达,其中在大脑中的相对表达量最高,肺和皮肤中的相对表达量最低;同时在渝东白羊皮肤中的相对表达量是酉州乌羊皮肤的3.44倍,且差异极显著(P<0.01);在细胞增殖过程中,miR-29a相对表达量极显著增加(P<0.01);在分化初期miR-29a相对表达量较高,之后趋于稳定且差异不显著(P>0.05)。在加入分化培养基后,细胞的形态发生变化,黑色素分泌增多。综上所述,miR-29a在酉州乌羊各组织中广泛表达,miR-29a的相对表达量随着B16细胞的增殖而递增,但随着B16细胞的分化而降低,推测miR-29a与B16细胞的增殖与分化密切相关。本研究结果为明确miR-29a在酉州乌山羊各组织中的表达特性及其是否参与了黑色素细胞行为学的调控提供了一定的理论证据。

关键词: miR-29a, 酉州乌羊, B16细胞, 表达规律, 形态学