Identification and Analysis of Aux/IAA Family Genes of Sanyueli (Prunus salicina Lindl.) and the Red-fleshed Mutant

doi:10.11869/j.issn.100-8551.2020.02.0247

Journal of Nuclear Agricultural Sciences ›› 2020, Vol. 34 ›› Issue (2): 247-255.DOI: 10.11869/j.issn.100-8551.2020.02.0247

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles Next Articles

Identification and Analysis of Aux/IAA Family Genes of Sanyueli (Prunus salicina Lindl.) and the Red-fleshed Mutant

FANG Zhizhen, JIANG Cuicui, ZHOU Danrong, PAN Shaolin, YE Xinfu^*

Fruit Research Institute, Fujian Academy of Agricultural Sciences/Fujian Engineering and Technology Research Center for Deciduous Fruit Trees, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013

Received:2019-05-26 Online:2020-02-10 Published:2019-12-13

三月李及其红肉突变体Aux/IAA家族基因鉴定及分析

方智振, 姜翠翠, 周丹蓉, 潘少霖, 叶新福^*

福建省农业科学院果树研究所/福建省落叶果树工程技术研究中心,福建福州 350013

通讯作者: 叶新福,男,研究员,主要从事作物品质遗传育种研究。E-mail:yexinfu@126.com
作者简介:方智振,男,副研究员,主要从事果树遗传育种与生物技术研究。E-mail:fzhzh2008@163.com
基金资助:
福建省自然科学基金项目(30471225),福建省省属公益类科研院所基本科研专项(2018R1013-1),福建省农业科学院青年英才计划项目(YC2015-9),福建省农业科学院创新团队项目(STIT2017-1-4)

Abstract

Abstract: To investigate the biological function of Aux/IAA family genes during fruit ripening in plum, bioinformatics methods were used to identify PsIAA family genes in fruit transcriptome of Sanyueli and its red-fleshed mutant. Additionally, the expression patterns of PsIAA genes were analyzed during ripening of plum fruits. Our results indicated that there were 26 Aux/IAA family members in fruit transcriptome of Sanyueli and its red-fleshed mutant. Most of them were predicted to be unstablely hydrophilic proteins located in the nucleus. Phylogenetic analysis revealed that PsIAA proteins were divided into two groups, A and B, containing 9 subgroups. Most of the PsIAA proteins contain 4 conserved domains. 16 PsIAA genes were differentially expressed during fruit ripening in Sanyueli and its red-fleshed mutant. The expression pattern of PsIAA11, PsIAA13, PsIAA14, PsIAA18, PsIAA19 and PsIAA25 in Sanyueli were significantly different from that in the red-fleshed mutant. Our results indicated that Aux/IAA genes were closely related to the ripening of plum fruits. These results may lay a foundation for further study on the function of Aux/IAA genes during ripening of plum fruits.

Key words: plum, transcriptome, Aux/IAA family, bioinformatics analysis, expression analysis

摘要： 为了解Aux/IAA家族基因在李果实成熟过程中的生物学功能,通过生物信息学鉴定三月李及其红肉突变体果实转录组中的Aux/IAA家族基因,并分析其在果实成熟过程中的表达模式。结果表明,三月李及其红肉突变体果实转录组中共有26个Aux/IAA家族成员,大部分为位于细胞核的不稳定亲水蛋白质。系统进化树分析表明,Aux/IAA家族成员可分为A、B 两组,共9个亚组。大部分Aux/IAA蛋白质含有4个高度保守的结构域。16个Aux/IAA家族基因在三月李及其红肉突变体果实成熟过程中差异表达。PsIAA11、PsIAA13、PsIAA14、PsIAA18、PsIAA19和PsIAA25在三月李及其红肉突变体果实成熟过程中的表达模式存在显著差异。上述结果表明,Aux/IAA家族基因与李果实成熟密切相关,这为深入研究Aux/IAA家族基因在李果实成熟过程中的功能奠定了基础。

关键词: 李, 转录组, Aux/IAA家族, 生物信息学分析, 表达分析

FANG Zhizhen, JIANG Cuicui, ZHOU Danrong, PAN Shaolin, YE Xinfu. Identification and Analysis of Aux/IAA Family Genes of Sanyueli (Prunus salicina Lindl.) and the Red-fleshed Mutant[J]. Journal of Nuclear Agricultural Sciences, 2020, 34(2): 247-255.

方智振, 姜翠翠, 周丹蓉, 潘少霖, 叶新福. 三月李及其红肉突变体Aux/IAA家族基因鉴定及分析[J]. 核农学报, 2020, 34(2): 247-255.

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创刊：1987年
主管部门：农业农村部
主办单位：中国原子能农学会与中国农业科学院农产品加工研究所（前中国农业科学院原子能利用研究所）
ISSN：1000-8551

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Identification and Analysis of Aux/IAA Family Genes of Sanyueli (Prunus salicina Lindl.) and the Red-fleshed Mutant

三月李及其红肉突变体Aux/IAA家族基因鉴定及分析

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