Journal of Nuclear Agricultural Sciences ›› 2019, Vol. 33 ›› Issue (5): 894-901.DOI: 10.11869/j.issn.100-8551.2019.05.0894

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles     Next Articles

Relationship Between Two Resistance Gene and Diseases in High Oleic Acid Rapeseed

WANG Xiaodan, CHEN Hao, ZHANG Zhenqian*, GUAN Chunyun   

  1. Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance of Hunan Province/Southem Regional Collaborative Innovation Center for Grain and Oil Crops, Hunan Agricultural University, Changsha, Hunan 410128
  • Received:2018-09-03 Online:2019-05-10 Published:2019-03-06

2个抗病相关基因与高油酸油菜病害关系分析

王晓丹, 陈浩, 张振乾*, 官春云   

  1. 湖南农业大学南方粮油作物协同创新中心/水稻油菜抗病育种湖南省重点实验室,湖南 长沙 410128
  • 通讯作者: *张振乾,男,副教授,主要从事油菜育种研究。E-mail:770204@163.com
  • 作者简介:王晓丹,女,主要从事油菜育种研究。E-mail:13838378556@163.com
  • 基金资助:
    国家973计划(2015CB150206),高油酸油菜抗病相关基因筛选及其功能验证(SYKB201701)

Abstract: In order to rapid screen strongly resistant high oleic acid rapeseed varieties, In this study, 20 high oleic acid rapeseed varieties were used as materials,the disease resistance of different materials was investigated,and disease-resistance related genes expression of HSP90 and ATG3 which screened from microRNA sequencing and detected by RT-qPCR, then the relationship between related genes and disease index were analyzed.The results showed that there was a significantly linear positive correlation between incidence and disease index of 20 high oleic acid rapeseed varieties,the expression of HSP90 decreased gradually until the flowering stage, and there were two kinds of expression patterns in the pod stage with Low - high - low and gradually decreased. ATG3 gene expression increased gradually until the flowering stage, and also appeared the same expression patterns with HSP90 in pod stage. When the expression of HSP90 was more than 1.5 times of the reference gene in florescence-leaf, the disease-resistant materials were screened with 95% accuracy. Similarly, when the expression of ATG3 was more than 1.8 times of the reference gene in florescence-flowers, the disease-resistant materials were screened with 80% accuracy. In addition, to combine the expression of HSP90 in florescence-leaf and ATG3 in 5-6 leaf stage leaves can also be used to predict the disease-resistant materials (80% accuracy). The results of this study provids a certain theoretical foundation for screening high oleic acid rapeseed materials with strong disease-resistance in the early stages and promoting the breeding of high oleic acid rapeseed.

Key words: high oleic acid rapeseed, resistance gene, expression pattern, diseases

摘要: 为快速筛选抗病性强的高油酸油菜品种,本研究以20个高油酸油菜品系为试验材料,调查不同材料的抗病情况,并通过RT-qPCR测定经miRNA测序筛选得到的与抗病相关基因HSP90和ATG3的表达情况,同时分析其与病情指数的关系。结果表明,20个高油酸油菜品系的发病率与病情指数呈线性正相关关系,且差异极显著;HSP90基因从苗期至花期表达量呈逐渐降低的趋势,角果期有先升高后降低和逐渐升高2种表达模式;ATG3基因从苗期至花期表达量也呈逐渐升高的趋势,角果期表达模式与HSP90基因相同。用HSP90基因在花期-叶中表达量≥1.5倍内参时进行抗病材料筛选,有95%的准确率;用ATG3基因在花期-叶中表达量≥1.8倍内参时进行抗病材料筛选,有80%的准确率,此外,也可用HSP90在花期-叶结合ATG3在五~六叶期-叶中表达情况进行预测,有80%的准确率。本研究结果为高油酸油菜抗病材料的早期筛选和油酸油菜育种提供了一定的理论依据。

关键词: 高油酸油菜, 抗病基因, 表达规律, 病害