Journal of Nuclear Agricultural Sciences ›› 2019, Vol. 33 ›› Issue (4): 759-765.DOI: 10.11869/j.issn.100-8551.2019.04.0759

• Food Irradiation·Food Science • Previous Articles     Next Articles

Comparative Analysis of Fatty Acid Compositions of Laminaria japonica From Different Origins

XU Hua, ZHANG Ru, ZHANG Jinjie, LOU Qiaoming*, YANG Wenge, XU Dalun   

  1. School of Marine Science, Ningbo University, Ningbo, Zhejiang 315211
  • Received:2017-08-18 Revised:2018-02-02 Online:2019-04-10 Published:2019-01-29

不同产地海带脂肪酸组成的比较分析

徐华, 张茹, 张进杰, 楼乔明*, 杨文鸽, 徐大伦   

  1. 宁波大学海洋学院,浙江 宁波 315211
  • 通讯作者: *楼乔明, 男, 副教授, 主要从事生物活性脂质研究。E-mail: louqm2005@163.com
  • 作者简介:徐华, 男, 主要从事生物资源开发利用研究。E-mail: xuhua1994@yeah.net
  • 基金资助:
    国家自然科学基金项目(31201283),国家星火计划项目(2014GA701010),“水产”浙江省重中之重学科开放基金项目(xkzsc1531)

Abstract: In order to analyze comparatively the fatty acid compositions of Laminaria japonica from difference origins, the total lipid extraction conditions of Laminaria japonica from three origins (Fuzhou, Ningbo and Qingdao) were optimized by solvent methods, and the fatty acid compositions were analyzed by GC-MS in this study. The results indicated that extraction with dichloromethane-methanol was an ideal method for lipid extraction from Laminaria japonica with high extraction efficiency. The total lipid contents of Laminaria japonica from Fuzhou, Ningbo and Qingdao were 0.75%, 0.69% and 0.86%, respectively, with no significant difference. Additionally, thirty kinds of fatty acids were identified from total lipids of Laminaria japonica, which were mainly composed of C16:0, C18:1n-9, C20:4n-6 (AA) and C20:5n-3 (EPA). Meanwhile, significant differences were existed in fatty acid compositions from different origins. The contents of C20:4n-6 and C20:5n-3 in Fuzhou samples were 10.12% and 6.18%, respectively, and the content of C20:4n-6 was significantly higher than those in Ningbo (9.41%) and Qingdao (8.77%), while the content of C20:5n-3 was significantly lower than those in Ningbo (8.13%) and Qingdao (9.40%). Hence, the indexes (n-3 PUFA, n-6 PUFA, n-3/n-6, EPA+AA and EPA/AA) of Laminaria japonica from three origins were significantly different. This research provided theoretical basis for total lipid extraction, fatty acid analysis and nutritional evaluation of Luminaria japonica rom different origins.

Key words: Luminaria japonica, total lipids, fatty acids, origin

摘要: 为比较分析不同产地海带的脂肪酸组成,本研究采用溶剂法对海带总脂提取条件进行优化,并通过气相色谱-质谱技术对福州、宁波和青岛3个产地海带的总脂脂肪酸组成进行比较分析。结果表明,二氯甲烷-甲醇法提取海带总脂的效率较高,是海带总脂提取的理想方法;福州、宁波和青岛海带的总脂含量依次为0.75%、0.69%和0.86%,且无显著差异。从海带总脂中共鉴定出30种脂肪酸,以 C16:0、C18:1n-9、C20:4n-6(AA)和C20:5n-3(EPA)为主,且3个产地海带在脂肪酸组成上存在显著差异。福州产地海带中的C20:4n-6和C20:5n-3含量分别为10.12%、6.18%,其中C20:4n-6含量显著高于宁波(9.41%)和青岛(8.77%),C20:5n-3含量显著低于宁波(8.13%)和青岛(9.40%),进而使3个产地海带中的n-3 PUFA、n-6 PUFA、n-3/n-6、EPA+AA和EPA/AA等指标存在显著差异。本研究结果为不同产地海带总脂提取、脂肪酸比较分析及营养评价提供了一定的理论依据。

关键词: 海带, 总脂, 脂肪酸, 产地