Journal of Nuclear Agricultural Sciences ›› 2019, Vol. 33 ›› Issue (3): 482-489.DOI: 10.11869/j.issn.100-8551.2019.03.0482

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles     Next Articles

Analysis of WD40 Protein Family Based on Transcriptome Sequencing in Lycium ruthenicum Murr.

YAN Li1, 2, CHEN Jianwei2, WANG Cuiping2, *, TONG Qian1, WANG Chen1, QIAO Gaixia2, LI Jian2   

  1. 1 College of Life Science, Ningxia University, Yinchuan, Ningxia 750021;
    2 Ningxia Forestry Institute Key Laboratory of the Seedling Bioengineering, Yinchuan, Ningxia 750004
  • Received:2017-11-13 Revised:2018-02-02 Online:2019-03-10 Published:2019-01-23

基于转录组信息的黑果枸杞WD40蛋白质家族分析

严莉1, 2, 陈建伟2, 王翠平2, *, 仝倩1, 王晨1, 乔改霞2, 李健2   

  1. 1 宁夏大学生命科学学院,宁夏 银川 750021;
    2 宁夏林业研究院种苗生物工程国家重点实验室,宁夏 银川 750004
  • 通讯作者: 王翠平,女,助理研究员,主要从事枸杞遗传育种研究。E-mail:wangcuipingcas@163.com
  • 作者简介:严莉,女,主要从事逆境植物生理生态学研究。E-mail:18295085526@163.com
  • 基金资助:
    国家自然科学基金(31701878、31660045),国际合作专项项目(2015DFA90900),宁夏林业科技重大公益枸杞专项(2016-328)

Abstract: WD40 protein is one of the most common transcription factor families in eukaryotes. It is widely involved in many biological processes and plays an important role in protein-protein and protein-DNA interactions. In order to systematically analyze the WD40 protein family members of Lycium ruthenicum Murr., the transcriptome sequencing data of the fruit in the green, colouring, black periods of L. ruthenicum Murr.. was analyzed with the Nr、Nt、Pfam、KOG/COG、Swiss-prot、KEGG and GO database and NCBI website to screen and annotate the encoding genes of WD40 protein in L. ruthenicum Murr. We have screened 77 members of WD40 protein family in L. ruthenicum Murr.. The structural domain of WD40 protein in L. ruthenicum Murr. is diverse, and which is highly conserved among different species. The results of the subcellular localization demonstrated WD40 protein family in L. ruthenicum Murr. was mostly located in the nucleus and cytoplasm. The phylogenetic trees of WD40 protein family of L. ruthenicum Murr. and Arabidopsis thaliana showed that WD40 protein family of L. ruthenicum Murr. contained five subfamilies. The expression WD40 genes of L. ruthenicum Murr. dependent furit development indicated that WD40 protein family members of L. ruthenicum Murr. may be involved in the regulation of anthocyanin in their fruit. This study systematically analyzed the members of WD40 protein family of L. ruthenicum Murr., which provide the basis for the study on the function and isolation of WD40 protein from Lycium barbarum Murr..

Key words: Lycium ruthenicum Murr., transcriptome sequencing, WD40 protein, hierarchical clustering analysis, expression pattern

摘要: WD40蛋白质为真核生物中一种常见蛋白质,广泛参与植物生长发育的多种生物过程,在蛋白质-蛋白质及蛋白质-DNA的相互作用中发挥重要作用。为系统分析黑果枸杞WD40蛋白质家族成员,本研究以黑果枸杞青色期、转色期和成熟期3个发育时期的果实转录组测序数据为基础,结合Nr、Nt、Pfam、KOG/COG、Swiss-prot、KEGG及GO 7个数据库和NCBI网站,对黑果枸杞WD40蛋白质编码基因进行筛选注释。结果表明,共得到77个黑果枸杞WD40蛋白质家族成员,黑果枸杞WD40蛋白质结构域组成多样,且在不同物种间高度保守;亚细胞定位预测分析表明,黑果枸杞WD40蛋白质家族成员多位于细胞核及细胞质中;黑果枸杞和拟南芥WD40蛋白质共建的系统进化树可分为五个亚家族;WD40蛋白质家族成员编码基因随果实发育的表达模式表明,黑果枸杞WD40蛋白质家族成员可能参与了其果实花青素的变化调控。本研究结果为枸杞WD40蛋白质的分离及功能研究奠定了一定的理论基础。

关键词: 黑果枸杞, 转录组测序, WD40蛋白, 分层聚类分析, 表达模式