Journal of Nuclear Agricultural Sciences ›› 2018, Vol. 32 ›› Issue (8): 1466-1476.DOI: 10.11869/j.issn.100-8551.2018.08.1466

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles     Next Articles

Effect of Atmospheric Room Temperature Plasma on Seeds Germination and Seedling Physiological Characteritics of Oil Flax Under NaCl Stress

ZHAO Dongxiao**, DU Jianxun**, DONG Yaru, SUN Jingshi, LIANG Xiaoyan, WANG Xiangyu, SHI Xinqin, GUO Hong′en*   

  1. The Sericultural Research Institute of Shandong Province,Yantai,Shandong 264002
  • Received:2017-08-18 Online:2018-08-10 Published:2018-06-30

常压室温等离子体对NaCl胁迫下胡麻种子萌发及幼苗生理特性的影响

赵东晓**, 杜建勋**, 董亚茹, 孙景诗, 梁晓艳, 王向誉, 施新琴, 郭洪恩   

  1. 山东省蚕业研究所,山东 烟台 264002
  • 通讯作者: 郭洪恩,男,研究员,主要从事温室大棚与植物栽培研究。E-mail:guohongen163@163.com
  • 作者简介:赵东晓,女,助理研究员,主要从事植物逆境生理以及分子生物学研究。E-mail:zhaodongxiao001@163.com;
    杜建勋,男,副研究员,主要从事植物育种与栽培研究。E-mail:dujianxun1970@126.com。**同为第一作者。
  • 基金资助:
    山东省农业科学院农业科技创新工程项目(CXGC2016B10、CXGC2016A03),国家自然科学基金(31500016),山东省农业重大应用技术创新项目(201706)

Abstract: To explore the effects of atmospheric temperature plasma (ARTP) treatment on salt tolerance of oil flax, 2 varieties of oil flax(Tian Ya 10 and Long Ya 13)were used as materials in this experiment. ARTP was used to irradiate flax seeds for different times (0, 10, 20, 30 min). Based on the treatment, the germination index, seedling growth index, chlorophyll (chl) and carotenoid content, leaf membrane permeability, leaf malondialdehyde (MDA) content, soluble protein content and superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity under salt stress with different concentrations of NaCl (0,85,170 mmol·L-1) were determined, and the influence of ARTP on seed germination and seedling growth of oil flax under salt stress were analyzed. The results showed that the effects of ARTP irradiation on two oil flax varieties were different. ARTP treatment improved germination and seedling growth of Tian Ya 10 under salt stress, which showed the decrease of membrane permeability, chlorophyll content, carotenoid content, MDA content and the increase of SOD, POD, CAT activity and soluble protein content. After treated by ARTP for 10min under the stress condition of 170 mmol· L-1 NaCl stress, the germination rate of Tian Ya 10 increased 90.27%, while the germination and seedling growth of Long Ya 13 was reduced, showing the increase of membrane permeability, chlorophyll content, carotenoid content, MDA content and decrease of SOD, POD, CAT activity and soluble protein content. After treated by ARTP for 30 min under 170 mmol· L-1 NaCl stress, the germination rate of Long Ya 13 declined by 17.60%. The results of this study provide a theoretical reference for oil flax breeding and artificial cultivation of saline alkali land.

Key words: Linum usitatissimum L., atmospheric room temperature plasma, salt stress, seed germination, physiological characteristic

摘要: 为探究常压室温等离子体(ARTP)照射处理对胡麻耐盐性的影响,以2个胡麻品种天亚10号和陇亚13号为材料,利用ARTP对胡麻种子进行不同时长(0、10、20、30 min)照射,测定不同NaCl浓度(0、85、170 mmol·L-1)胁迫下胡麻的发芽指标、幼苗生长指标、叶绿素(Chl)和类胡萝卜素含量、叶片质膜相对透性、叶片丙二醛(MDA)含量、可溶性蛋白含量及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,分析ARTP处理对NaCl胁迫下胡麻种子萌发及幼苗生长的影响。结果表明,ARTP处理对2个胡麻品种的影响存在差异,ARTP处理可以促进天亚10号在NaCl胁迫下种子萌发和幼苗生长,降低质膜相对透性、叶绿素含量、类胡萝卜素含量、MDA含量,提高POD活性及可溶性蛋白含量;在170 mmol·L-1 NaCl胁迫下ARTP处理10 min,天亚10号发芽率可以提高90.27%;但抑制了陇亚13号在NaCl胁迫下种子萌发和幼苗生长,增加了质膜相对透性、叶绿素含量、类胡萝卜素含量、MDA含量,降低了POD活性及可溶性蛋白含量,在170 mmol·L-1 NaCl胁迫下ARTP处理30 min,陇亚13号的发芽率下降了17.60%。本研究结果为胡麻育种及盐碱地人工栽培提供了一定的理论参考。

关键词: 胡麻, 常压室温等离子体, 盐胁迫, 种子萌发, 生理特性