Journal of Nuclear Agricultural Sciences ›› 2018, Vol. 32 ›› Issue (7): 1338-1348.DOI: 10.11869/j.issn.100-8551.2018.07.1338

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles     Next Articles

Genome-wide Characterization of MYB Transcription Factors and Response to Abiotic Stresses in Ipomoea triloba

SHI Xiaowen1, HE Liheng1, JIAO Jinhua2, LIU Xiayu1, WANG Ting1, LIU Shifang1, JIA Xiaoyun1, *, LI Runzhi1, *   

  1. 1 College of Life Science, Shanxi Agricultural University, Taigu, Shanxi 030801;
    2 Pomology Institute, Shanxi Academy of Agricultural Sciences, Taigu, Shanxi 030815
  • Received:2018-03-05 Revised:2018-05-02 Online:2018-07-10 Published:2018-05-16

甘薯二倍体近缘野生种三裂叶薯MYB转录因子全基因组分析及逆境胁迫响应

石晓雯1, 贺立恒1, 焦晋华2, 刘霞宇1, 王婷1, 刘世芳1, 贾小云1, *, 李润植1, *   

  1. 1 山西农业大学生命科学学院,山西 太谷 030801;
    2山西省农业科学院果树研究所,山西 太谷 030815
  • 作者简介:石晓雯,女,主要从事MicroRNA在植物花青素代谢及逆境胁迫响应中的功能研究。E-mail: 291789619@qq.com
  • 基金资助:
    山西省人才引进与开发专项(614191),山西省农业科技成果转化和推广示范项目(2018-5),2018年山西农业大学科技创新基金,山西省煤基重点科技攻关项目(FT-2014-01)

Abstract: MYB is one of the most important transcription factors in eukaryotic, which is involved in regulating plant growth and development, primary and secondary metabolites and biotic and abiotic stress. In order to comprehensive genome-weide analysis of the MYB transcription fator in sweet potato. In this study, genome-wide classification and analysis of MYB transcription factor family were conducted based on the genome sequences of Ipomoea triloba (I.triloba), a wild the diploid progenitor of sweet potato. The conservative structure domain, gene structure, conserved motif, chromosome localization and phylogenetic relation were identified with multiple bioinformatic tools and databases online. The expression patterns of 10 R2R3-MYB genes under different treatments of drought and salt stresses were detected by qRT-PCR. The results showed a total of 160 ItbMYB were identified, which were distributed unequally along 15 chromosomes, MYB genes on the 7th was most (21 genes), and that on the 8th was least(6 genes). According to repeat number of the MYB domain, the 160 ItbMYB transcription factors were classified into 4 groups, including 1R-MYB (36 genes), R2R3-MYB (120 genes), 3R-MYB (4 genes) and 4R-MYB (1 gene). The result of phylogenetic analysis revealed that the 161 ItbMYB genes were divided into 18 subfamilies. In the same ItbMYB subfamily, the composition and number of motif were highly conserved, but the number of exons and introns varied highly. Based on the classification criteria of Arabidopsis R2R3-MYB, the 120 R2R3-MYB transcription factors were further divided into 30 subgroups. The qRT-PCR results showed that ItbMYB genes responded to drought and salt stress, and the expression levels were different in different tissues under salt and drought stresses. The study provides a foundation for further function identification of MYB genes in sweet potato.

Key words: Ipomoea triloba, MYB transcription factor, genome-wide analysis, abiotic stresses, expression analysis

摘要: MYB是真核生物中一类重要的转录因子,参与调控植物生长发育、初生次生代谢和生物或非生物胁迫响应。为全面解析甘薯基因组MYB转录因子信息,本研究基于甘薯二倍体近缘野生种三裂叶薯的全基因组测序数据,利用多种生物学软件和在线工具对三裂叶薯MYB转录因子的结构域、基因结构、保守基序、染色体定位和系统进化等进行鉴定和分析。利用qRT-PCR检测其中10个R2R3-MYB基因在干旱和盐胁迫下的表达情况。结果表明,在三裂叶薯的全基因组中共鉴定出不均匀分布于15条染色体的160个ItbMYB基因,其中第7号染色体上分布最多(21个基因),第8号染色体上分布最少(6个基因)。根据MYB结构域所含MYB重复个数,160个ItbMYB家族转录因子被分为4类,其中1R类包含36个基因、R2R3类120个基因、3R类4个基因、4R类1个基因。系统进化分析显示,160个ItbMYB聚为18个亚家族,且同一亚家族的ItbMYB转录因子的motif类型和数目相似,但所含外显子和内含子的数目不同。根据拟南芥R2R3-MYB转录因子的分类标准,进一步将三裂叶薯R2R3-MYB类分为30个亚类。qRT-PCR检测结果表明,R2R3-MYB响应干旱和盐胁迫,且不同胁迫时间下不同组织中的表达量存在差异。本试验为进一步研究甘薯MYB转录因子的功能奠定了一定的理论基础。

关键词: 三裂叶薯, MYB转录因子, 基因组鉴定, 逆境胁迫, 表达分析