The Gynura bicolor Transcriptome as A Source for Anthocyanidin Gene Sequence Analysis

doi:10.11869/j.issn.100-8551.2018.04.0639

Journal of Nuclear Agricultural Sciences ›› 2018, Vol. 32 ›› Issue (4): 639-645.DOI: 10.11869/j.issn.100-8551.2018.04.0639

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The Gynura bicolor Transcriptome as A Source for Anthocyanidin Gene Sequence Analysis

ZHANG Shaoping^{1, 3}, ZHANG Shaohua², QIU Shanlian^{1, *}, ZHANG Shuai¹, WU Songhai¹, HE Yansen¹, ZHENG Kaibin^{1, 3}, ZHENG Jiaxie¹

1 Subtropical Agriculture Research Institute, Fujian Academy of Agricultural Sciences, Zhangzhou, Fujian 363005;
2 Ningbo Institute for Food Control, Ningbo, Zhejiang 315048;
3 Crop Sciences Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013

Received:2017-04-12 Online:2018-04-10 Published:2018-02-07
Supported by:
福建省公益类科研院所专项(2016R1012-4,2017R1024-3),福建省农业科学院青年英才计划项目(YC2015-19),福建省农业科学院青年创新团队项目(STIT2017-3-4)

基于转录组测序的紫背天葵花青素相关基因分析

张少平^{1, 3}, 张少华², 邱珊莲^{1, *}, 张帅¹, 吴松海¹, 何炎森¹, 郑开斌^{1, 3}, 郑加协¹

1 福建省农业科学院亚热带农业研究所,福建漳州 363005;
2 宁波市食品检验检测研究院,浙江宁波 315048;
3 福建省农业科学院作物研究所,福建福州 350013

通讯作者: 邱珊莲,女,副研究员,主要从事特色植物功能成分相关研究。E-mail:slqiu79@163.com
作者简介:张少平,男,农艺师,主要从事特色蔬菜相关研究。E-mail:zspnc@163.com

Abstract

Abstract:

In order to explore Gynura bicolor correlated genes in biosynthesis pathway of anthocynidin under the normal conditions, this study selected all part of Gynura bicolor as experimental materials, the transcriptome of Gynura bicolor was sequenced by Illumina HiSeq 2500 platform. 29 Unigenes related to anthocyanidin were obtainded by 3 databases including GO, Swiss-Prot and NR, which belonged to 4 kinds of genes involved 17 anthocyanidin relevant glucosyltransferases,3 anthocyanidin relevant acyltransferases, 6 anthocyanidin relevant reductases and 3 leucoanthocyanidin dioxygenases. The clustering analysis from 29 anthocyanidin Unigenes containing 4 kins of genes showed that anthocyanin acyltransferases had the closest relationship, followed by leucoanthocyanidin dioxygenase anthocyanin acyltransferases, then anthocyanin relevant reductases,and the genetic relation among anthocyanidin relevant glucosyltransferases is the farthest.The 29 unigenes related to anthocyanins biosynthesis are helpful for further research to anthocyanin biosynthesis process and gene cloning from Gynura bicolor.

Key words: Gynura bicolor, transcriptome sequencing, function annotation, anthocyanidin gene

摘要：

为获取正常生长状态下紫背天葵花青素合成相关基因,本研究以全株紫背天葵为试验材料,采用高通量测序技术(Illumina HiSeq 2500)对紫背天葵全转录功能基因组测序后组装,再通过GO、Swiss-Prot和NR三大数据库进行花青素相关功能基因筛选,共获得29个花青素相关Unigene,包括17个花青素相关糖基转移酶、3个花青素酰基转移酶、6个花青素相关还原酶以及3个无色花色素双加氧酶(合酶)。聚类分析研究表明,涉及到花青素合成相关的四类酶成员的29个Unigene中,花青素酰基转移酶家族内部之间亲缘关系最近,无色花色素双加氧酶次之,花青素相关还原酶亲缘关系稍远,而花青素相关糖基转移酶家族成员亲缘关系最远。本研究获取的29个紫背天葵花青素相关Unigene信息,为进一步研究紫背天葵花青素生物合成过程及基因克隆等奠定了理论基础。

关键词: 紫背天葵, 转录组测序, 功能注释, 花青素基因

ZHANG Shaoping, ZHANG Shaohua, QIU Shanlian, ZHANG Shuai, WU Songhai, HE Yansen, ZHENG Kaibin, ZHENG Jiaxie. The Gynura bicolor Transcriptome as A Source for Anthocyanidin Gene Sequence Analysis[J]. Journal of Nuclear Agricultural Sciences, 2018, 32(4): 639-645.

张少平, 张少华, 邱珊莲, 张帅, 吴松海, 何炎森, 郑开斌, 郑加协. 基于转录组测序的紫背天葵花青素相关基因分析[J]. 核农学报, 2018, 32(4): 639-645.

References

[1] 张少平, 邱珊莲, 邓源, 郑云云, 赖正锋, 陈玉水, 郑加协.紫背天葵花青素相关研究与应用[J]. 中国农学通报, 2015, 31(22): 157-162
[2] Chen J, Mangelinckx S, Lü H, Wang Z T, Li W L, De Kimpe N. Profiling and elucidation of Gynura bicolor and Gynura divaricata collected from different Chinese origins[J]. Chemistry and Biodiversity, 2015, 12(1): 96-115
[3] Miyazawa M, Nakahashi H, Usami A, Matsuda N. Chemical composition, aroma evaluation, and inhibitory activity towards acetylcholinesterase of essential oils from Gynura bicolor D C[J]. Journal of Natural Medicine, 2016, 70(2): 282-289
[4] Teoh W Y, Tan H P, Ling S K, Abdul W N, Sim K S. Phytochemical investigation of Gynura bicolor leaves and cytotoxicity evaluation of the chemical constituents against HCT 116 cells[J]. Natural Product Research, 2016, 30(4): 448-451
[5] Springob K, Nakajima J, Yamazaki M, Saito K. Recent advances in the biosynthesis and accumulation of anthocyanins[J]. Natural Product Reports, 2003, 20(3): 288-303
[6] Bagchi D, Sen C K, Bagchi M, Atalay M.Anti-angiogenic, antioxidant, and anti-carcinogenic properties of a novel anthocyanin-rich berry extract formula[J]. Biochemistry Biokhimiia, 2004, 69(1): 75-80
[7] Chao C Y, Liu W H, Wu J J, Yin M C. Phytochemical profile,antioxidative and anti-inflammatory potentials of Gynura bicolor D C[J]. Journal of the Science of Food and Agriculture, 2015, 95(5): 1088-1093
[8] Wu C C, Chang Y P, Wang J J, Liu C H, Wong S L, Jiang C M, Hsieh S L. Dietary administration of Gynura bicolor (Roxb. Willd.) D C water extract enhances immune response and survival rate against Vibrio alginolyticus and white spot syndrome virus in white shrimp Litopeneaus vannamei [J]. Fish and Shellfish Immunology, 2015, 42(1): 25-33
[9] Wang M, Liu H, Dong C, Fu Y, Liu H. Elevated CO 2 enhances photosynthetic efficiency, ion uptake and antioxidant activity of Gynura bicolor D C. grown in a porous-tube nutrient delivery system under simulated microgravity[J]. Plant Biology, 2016, 18(3): 391-399
[10] Shimizu Y, Maeda K, Kato M, Shimomura K.Isolation of anthocyanin-related MYB gene, GbMYB2, from Gynura bicolor l eaves[J]. Plant Biotechnology, 2010, 27(7): 481-487
[11] Shimizu Y, Maeda K, Kato M, Shimomura K.Co-expression of GbMYB1 and GbMYC1 induces anthocyanin accumulation in roots of cultured Gynura bicolor D C. plantlet on methyl jasmonate treatment[J]. Plant Physiology and Biochemistry, 2011, 49(2): 159-167
[12] Zhang Y,Liu Z,Liu J,Lin S,Wang J,Lin W,Xu W.GA-DELLA pathway is involved in regulation of nitrogen deficiency-induced anthocyanin accumulation[J].Plant Cell Reports,2017, 36(4): 557-569
[13] Butelli E, Garcia-Lor A, Licciardello C, Las Casas G, Hill L, Recupero G R, Keremane M L,Ramadugu C,Krueger R, Xu Q, Deng X, Fanciullino A L, Froelicher Y, Navarro L, Martin C.Changes in anthocyanin production during domestication of Citrus [J].Plant Physiology,2017, 173(4): 2225-2242
[14] Shimizu Y, Maeda K, Kato M, Shimomura K. Methyl jasmonate induces anthocyanin acculation in Gynura bicolor culture roots[J]. In Vitro Cellular and Developmental Biology Plant, 2010, 46(46): 460-465
[15] Gao C, Li D, Jin C, Duan S, Qi S, Liu K, Wang H, Ma H, Hai J, Chen M.Genome-wide ide.pngication of GLABRA3 downstream genes for anthocyanin biosynthesis and trichome formation in Arabidopsis [J].Biochemical and Biophysical Research Communication, 2017, 485(2): 360-365
[16] Li S,Wang W,Gao J,Yin K,Wang R,Wang C,Petersen M, Mundy J,Qiu J L.MYB75 phosphorylation by MPK4 is required for light-induced anthocyanin accumulation in Arabidopsis [J].Plant Cell, 2016, 28(11): 2866-2883
[17] Jin X,Huang H, Wang L,Sun Y,Dai S.Transcriptomics and metabolite analysis reveals the molecular mechanism of anthocyanin biosynthesis branch pathway in different senecio cruentus cultivars[J].Frontiers in Plant Science, 2016, 7(9): 1301-1307
[18] Sangwan R S, Tripathi S, Singh J, Narnoliya L K, Sangwan N S. De novo sequencing and assembly of Centella asiatica leaf transcriptome for mapping of structural, functional and regulatory genes with special reference to secondary metabolism[J]. Gene, 2013, 525(2): 58-76
[19] Campos-Parra A D, Padua-Bracho A, Pedroza-Torres A, Figueroa-González G, Fernández-Retana J, Millan-Catalan O, Peralta-Zaragoza O, Cantú de León D, Herrera L A, Pérez-Plasencia C. Comprehensive transcriptome analysis ide.pngies pathways with therapeutic potential in locally advanced cervical cancer[J]. Gynecol Oncol, 2016, 143(2): 406-413
[20] Xu L, Yang P, Yuan S, Feng Y, Xu H, Cao Y, Ming J. Transcriptome analysis ide.pngies key candidate genes mediating purple ovary coloration in Asiatic Hybrid Lilies[J]. International Journal of Molecular Sciences, 2016, 17(11): 1881-1888
[21] Nguyen C T, Lim S, Lee J G, Lee E J.VcBBX, VcMYB21 and VcR2R3MYB transcription factors are involved in UV-B-induced anthocyanin biosynthesis in the peel of harvested blueberry fruit[J].Journal Agriculture and Food Chemistry, 2017, 65(10): 2066-2073
[22] 张少平, 洪建基, 邱珊莲, 郑云云, 张帅, 吴松海, 何炎森, 郑开斌, 郑加协. 紫背天葵高通量转录组测序分析[J]. 园艺学报, 2016, 43(5): 935-946
[23] 张少平, 邱珊莲, 郑云云, 张帅, 吴松海, 何炎森, 郑开斌. 紫色黄秋葵转录组功能基因测序及分析[J].核农学报, 2017, 31(4): 643-653
[24] Jia X, Shen J, Liu H, Li F, Ding N, Gao C, Pattanaik S, Patra B, Li R, Yuan L. Small tandem target mimic-mediated blockage of microRNA858 induces anthocyanin accumulation in tomato[J]. Planta, 2015, 242(1): 283-293
[25] Chen Q,Man C,Li D,Tan H,Xie Y,Huang J.Arogenate dehydratase isoforms differentially regulate anthocyanin biosynthesis in Arabidopsis thaliana [J].Molecular Plant, 2016, 9(12): 1609-1619
[26] Li W, Wang B, Wang M, Chen M, Yin J M, Kaleri G M, Zhang R J, Zuo T N, You X, Yang Q. Cloning and characterization of a potato StAN 11 gene involved in anthocyanin biosynthesis regulation[J]. Journal Integrative Plant Biology, 2014, 56(4): 364-372
[27] Hsu Y H,Tagami T,Matsunaga K,Okuyama M,Suzuki T,Noda N,Suzuki M,Shimura H.Functional characterization of UDP-rhamnose-dependent rhamnosyltransferase involved in anthocyanin modification,a key enzyme determining blue coloration in Lobelia erinus[J]. Plant Journal, 2017, 89(2): 325-337
[28] Lim S H, You M K, Kim D H, Kim J K, Lee J Y, Ha S H. RNAi-mediated suppression of dihydroflavonol 4-reductase in tobacco allows fine-tuning of flower color and flux through the flavonoid biosynthetic pathway[J]. Plant Physiology and Biochemistry, 2016, 109(10): 482-490
[29] Morita Y, Ishiguro K, Tanaka Y, Iida S, Hoshino A. Spontaneous mutations of the UDP-glucose: flavonoid 3-O-glucosyltransferase gene confers pale- and dull-colored flowers in the Japanese and common morning glories[J]. Planta, 2015, 242(3): 575-587
[30] Huai G, Qi P, Yang H, Wang Y. Characteristics of α-Gal epitope, anti-Gal antibody, α-1,3 galactosyltransferase and its clinical exploitation[J]. Internation Journal of Molecular Medicine, 2016, 37(1): 11-20

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创刊：1987年
主管部门：农业农村部
主办单位：中国原子能农学会与中国农业科学院农产品加工研究所（前中国农业科学院原子能利用研究所）
ISSN：1000-8551

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The Gynura bicolor Transcriptome as A Source for Anthocyanidin Gene Sequence Analysis

基于转录组测序的紫背天葵花青素相关基因分析

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