Effect of 60Co-γ Irradiation on Capsule of Epidendium secundum

doi:10.11869/j.issn.100-8551.2017.09.1693

Journal of Nuclear Agricultural Sciences ›› 2017, Vol. 31 ›› Issue (9): 1693-1699.DOI: 10.11869/j.issn.100-8551.2017.09.1693

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles Next Articles

Effect of ⁶⁰Co-γ Irradiation on Capsule of Epidendium secundum

ZHOU Yaqian¹, YAO Na¹, WEI Li², LI Lubin¹, LIU Lei^{1, *}

1Research institute of Forestry, Chinese academy of forestry, Beijing 100091;
2 Environmental Monitoring Center of Handan, Handan, Hebei 056002

Received:2016-08-17 Online:2017-09-10 Published:2017-07-18

⁶⁰Co-γ射线对树兰蒴果辐照生物学效应研究

周亚倩¹, 姚娜¹, 魏莉², 李潞滨¹, 刘蕾^{1, *}

1中国林业科学研究院林业研究所,北京 100091;
2 邯郸市环境监测中心站,河北邯郸 056002

通讯作者: 刘蕾,女,助理研究员,主要从事园林植物与观赏园艺研究。E-mail:liulei0828@126.com
作者简介:周亚倩,女,主要从事园林植物与观赏园艺研究。E-mail:382373549@qq.com
基金资助:
国家科技支撑计划(2012BAD01B0702)

Abstract

Abstract: To investigate the effect of the ⁶⁰Co-γ ray treatment on the Epidendrum secundum capsule, different radiation doses of ⁶⁰Co-γ ray (0 to 200 Gy) were used in this study, and the effect of radiation on the seeds germination and the seedling growth were investigated. The results showed that 20 Gy radiation dose can promote the germination rate compared with the control group, and the germination time was shortened. Under the radiation dose of 200Gy, the seeds could not germinate. The semi-lethal dose (LD₅₀) was 78.08 Gy. The biological statistics analysis indicated the radiation dose had larger impact on plant height and leaf length, and had little effect on blade quantity and less influence on the leaf width. These results will lay the theory basis for radiation breeding of Epidendium secundum.

Key words: 60Co-γ, ray, Epidendrum secundum, capsule, mutation breeding, semi-lethal dose

摘要： 为探讨辐照处理对树兰种子的生物学效应,以树兰蒴果为研究对象,⁶⁰Co-γ射线为辐照源,设置不同剂量(0~200 Gy)进行辐照处理, 在组织培养条件下研究其生物学效应。结果表明,当辐照剂量为20 Gy时可提高种子萌发率,并缩短种子萌发时间,而当辐照剂量为200 Gy时,种子不再萌发;树兰种子的半致死剂量为78.08 Gy。辐照处理对树兰再生植株的影响明显,与辐照剂量呈正相关,对株高、叶长影响最大,叶片数量次之,叶宽最小。本研究结果为树兰属植物离体诱变育种提供了一定的科学依据。

关键词: 60Co-γ, 射线, 树兰, 蒴果, 诱变育种, 半致死剂量

ZHOU Yaqian, YAO Na, WEI Li, LI Lubin, LIU Lei. Effect of ⁶⁰Co-γ Irradiation on Capsule of Epidendium secundum[J]. Journal of Nuclear Agricultural Sciences, 2017, 31(9): 1693-1699.

周亚倩, 姚娜, 魏莉, 李潞滨, 刘蕾. ⁶⁰Co-γ射线对树兰蒴果辐照生物学效应研究[J]. 核农学报, 2017, 31(9): 1693-1699.

References

[1] Pinheiro F, De Barros F. Morphometric analysis of Epidendrum secundu m (Orchidaceae) in southeastern Brazil [J].Nordic Journal of Botany,2007, 25(3/4): 129-136
[2] Givnish T J, Spalink D, Ames M, Hunter S J, Zuluaga A, Doucette A, Caro G G, McDaniel J, Clements M A, Arroyo M T, Endara L, Kriebel R, Williams N H, Cameron K M. Orchid historical biogeography, diversification, antarctica and the paradox of orchid dispersal[J]. Journal of Biogeography, 2016, 43(10):1905-1916
[3] 黄永相, 郭涛, 蔡金洋, 刘永柱, 张建国, 王慧, 陈志强. 空间环境和 60 Co-γ辐照对水稻稻米品质的诱变效应[J]. 核农学报, 2013, 27(6): 709-714
[4] 彭振英, 王兴军, 田海莹, 郑玲, 单雷, 范仲学, 边斐, 郭峰, 王莹莹, 万书波. 花生 60 Co-γ辐照诱变和突变体库的构建[J]. 核农学报, 2016, 30(3): 422-429
[5] 陆波, 郑玉红, 陈默, 彭峰, 束晓春, 高风. 60 Co-γ射线对彩色马蹄莲Parfait的辐照效应及其在高温高湿胁迫下的生理响应[J]. 核农学报, 2014, 28(8): 1353-1357
[6] 刘军丽, 沈红香, 高遐红, 姚允聪. 60 Co-γ辐照对苹果属观赏海棠诱变效应的研究初报[J]. 中国农学通报, 2009, 25(8): 223-226
[7] 何平, 李林光, 王海波, 李慧峰, 常源升, 程来亮. 辐照处理对苹果嫩枝变异效应研究[J]. 核农学报, 2016, 30(7): 1249-1254
[8] 李静, 卢晓鹏, 邓钢桥, 熊江, 曹雄军, 谢深喜. 60 Co-γ射线对枳生物性状和分子特性的影响[J]. 核农学报, 2015, 29(7): 1239-1245
[9] 陈睿, 鲜小林, 万斌, 秦帆. 60 Co-γ辐照对两个杜鹃品种主要性状的影响[J]. 北方园艺, 2015, 39(2): 46-50
[10] 王磊. 丰花月季组培苗辐照诱变研究及遗传变异研究[D]. 哈尔滨: 东北农业大学, 2007:26-27
[11] 蒋彧, 何俊蓉, 刘菲, 王海娥, 卓碧萍. 60 Co-γ辐照兰花春剑隆昌素根状茎分化苗的ISSR分析[J]. 核农学报, 2013, 27(9): 1247-1252
[12] 林兵, 陈诗林, 黄敏玲, 钟淮钦, 陈源泉, 吴建设, 叶秀仙. 60 Co-γ射线辐照对3种国兰生长的影响[J]. 核农学报, 2009, 23(2): 244-247
[13] 洪萨丽, 金青, 黄蓓,蔡永萍, 林毅. 60 Co-γ辐照对霍山石斛悬浮培养原球茎生长和生物碱积累的影响[J]. 核农学报, 2009, 23(4):592-596
[14] 张相锋, 任艳丽, 尚天翠, 张维. 60 Co-γ射线辐照对蝴蝶兰原球茎生长的影响[J]. 北方园艺, 2009, 33(3): 177-180
[15] 李婧嫄. 60 Co-γ射线对寒兰根状茎的辐照诱变效应研究[D].南昌: 南昌大学, 2011
[16] 任羽, 张银东, 徐世松, 黄少华, 张志群. 60 Co-γ射线对石斛兰辐照效应的影响[J]. 热带作物学报, 2013, 34(9): 1672-1675
[17] 王慧娟, 孟月娥, 赵秀山, 张强, 李艳敏. 60 Co-γ射线辐照万寿菊对发芽率及生长的影响[J]. 中国农学通报, 2009, 25(19): 161-163
[18] 苏美和, 赵兰勇, 房涛. 60 Co-γ射线对牡丹种子发芽率及幼苗生长的影响[J]. 农学学报, 2012, 2(2): 18-21
[19] 聂硕, 张林, 王峰, 孙忠奎, 朱翠翠, 谢宪, 王长宪. 紫薇种子辐照变异和抗性初步研究[J]. 农学学报, 2016, 6(5):47-52
[20] 蔡春菊, 高健, 牟少华. 60 Co-γ辐照对毛竹种子活力及早期幼苗生长的影响[J]. 核农学报, 2007, 21(5): 436-440
[21] 包建忠, 李风童, 孙叶, 刘春贵, 马辉, 张甜, 陈秀兰. 60 Co-γ射线辐照大花君子兰种子对其萌发特性其开花性状的影响[J]. 核农学报, 2013, 27(11):1681-1685
[22] 许燕, 卢丽平, 纪鹏伟,陈炳华. 兰科植物种子数的科学计数法[J]. 生物学通报, 2015, 50(8):49-50
[23] 詹忠根, 徐程, 席芳. 137 Cs-γ射线辐照对铁皮石斛种胚原球茎的诱变研究[J]. 核农学报, 2009, 23(5): 816-819
[24] 熊冬梅, 曾璐, 熊兴耀, 田开忠, 刘阳, 石琢, 苏小军. 基于 60 Co-γ射线辐照的木糖乙醇发酵差异性突变菌株筛选及其发酵特性[J]. 核农学报, 2015, 29(2): 290-295
[25] 孔福全, 王潇, 倪嵋楠, 隋丽, 杨明建, 赵葵. γ射线诱导DNA 损伤中DNA 浓度和剂量率的影响[J]. 原子核物理评论, 2007, 24(2): 103-107
[26] 耿兴敏, 王良桂, 李娜, 杨秀莲. 60 Co-γ辐照对桂花种子萌发及幼苗生长的影响[J]. 核农学报, 2016, 30(2): 216-233
[27] 王月华 ,韩烈保, 尹淑霞, 王金来, 程晓霞. 60 Co-γ射线辐照对高羊茅种子发芽及酶活性的影响[J]. 核农学报, 2006, 20(3): 199-201
[28] 任羽, 潘丙成, 陆顺教, 张志群, 孔霞. 60 Co-γ辐照石斛兰组培苗的SRAP分析[J]. 中国农学通报, 2016, 32(31):85-89

Metrics

Comments

创刊：1987年
主管部门：农业农村部
主办单位：中国原子能农学会与中国农业科学院农产品加工研究所（前中国农业科学院原子能利用研究所）
ISSN：1000-8551

访问统计
总访问：
今日访问：
当前在线：

Effect of ⁶⁰Co-γ Irradiation on Capsule of Epidendium secundum

⁶⁰Co-γ射线对树兰蒴果辐照生物学效应研究

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	XU Wenya, ZHU Yuyan, XU Qihang, LI Sheng'e, SHEN Shuling, YAO Lanying, ZHENG Xiaolin. Eeffect of Umbelliferone Treatment on Disease Resistance to Botrytis cinerea in Kiwifruit cv. Xuxiang During Storage [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(4): 846-853.
[2]	LIU Fengluan, QIN Mi, YU Sheng, LIU Qingqing, ZHANG Dasheng, TIAN Daike. Effects of ⁶⁰Co-γ Irradiation on Lotus Seeds and Morphological Analysis of a Mutant With Narrow Tepals [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(12): 2681-2687.
[3]	ZHANG Li, ZHANG Yong, LEI Xingyu, ZHANG Yuanhai, ZHANG Yiyan, PENG Xuanming, YANG Zhen. Analysis of Small RNA Sequencing Variation in Rice Irradiated by ⁶⁰Co-γ Ray [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(11): 2441-2450.
[4]	WU Ping, GUO Junxia, WANG Xiaoyu, LI Qingmiao, ZHANG Songlin, LI Weijian, PENG Yu. Research on the Mutagenic Effects on Rhizome of Curcuma longa L. by ⁶⁰Co-γ Rays [J]. Journal of Nuclear Agricultural Sciences, 2021, 35(1): 1-6.
[5]	XU Yuanfang, LI Wenge, DENG Gangqiao, CHEN Shixin, ZHANG Qiling, ZHOU Yiji, ZHANG Yong, PENG Ling. Effect of ⁶⁰Co-γ Ray and Electron Beam Irradiation Sterilization on Antioxidant Activity and Fingerprint of Pueraria Extract [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(8): 1713-1721.
[6]	ZHAN Jin, CHONG Peifang, XIE Huimin, JIA Xiangyang, SHEN Yongtao. Physiological Response of Walnut Seedlings to Simulated Rainfall Changes [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(6): 1311-1319.
[7]	QI Wenquan, JIANG Zhenwei, WANG Yaqin, YANG Biao, LIU Bin, HE Yanhong, GAO Hongsheng. Radiation Effects of ⁶⁰Co-γ Ray on the Cutting Seedlings of Nine Garden Rose [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(4): 681-690.
[8]	LUO Qin, LI Dongmei, HUANG Minmin, RAO Qiuhua, LIU Yang, WENG Boqi, PAN Wei, LUO Tuyan. Fatty Acid Compositions and Principal Component Analysis in Muscle of Murray Cod at Different Growth Stages [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(4): 788-795.
[9]	CHEN Huizhe, XIANG Jing, WANG Yuejun, XU Yicheng, CHEN Yeping, ZHANG Yuping, ZHANG Yikai, ZHU Defeng. Seedlings Emergence Characteristics and Transplanting Quality Using the Tray-Overlaying Seedlings Raising Mode in Rice Mechanized Transplanting Systems [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(12): 2823-2830.
[10]	SHI Mingwei, PAN Hong, YAN Jianing, JIN Qijiang, WANG Yanjie, XU Yingchun. Biological Effect of ⁶⁰Co-γ Ray on Water Lily Tubers [J]. Journal of Nuclear Agricultural Sciences, 2020, 34(10): 2125-2132.
[11]	LI Yirui, WANG Dan, ZHAN Xiaodie, XU Chenghang, XU Longshui, DENG Shanwen, CHEN Hao. Responds of Electron Beam to Target X-Ray Irradiation onBio-effect of Gladiolus gandavensis and Evaluation of Radiosensitivity [J]. Journal of Nuclear Agricultural Sciences, 2019, 33(6): 1049-1058.
[12]	WU Jianhui, NIU Zhe, LAN Feng, ZHANG Jing. Effect of ⁶⁰Co-γ Ray on Physiological and BiochemicalCharacteristics of Two Kinds of Chrysanthemum grandi florum leaves [J]. Journal of Nuclear Agricultural Sciences, 2019, 33(6): 1059-1064.
[13]	QI Hui, WU Xiaofen, CHEN Liang, DENG Ming, WANG Keqin. The Enzymatic Hydrolysis Conversion Rate of Cellulose in RiceStraw Improved by Using NaOH Solution Collaborated With Irradiation [J]. Journal of Nuclear Agricultural Sciences, 2019, 33(6): 1190-1198.
[14]	ZHOU Ranran, GAO Hong, FAN Xiuzhi, YIN Chaomin, CHEN Zheya, YAO Fen, CHENG Wei, SHI Defang. Preliminary Comparison of Preservation Effect of Fresh Lentinus Edodes by ⁶⁰Co-γ Ray and Electron Beam Irradiation [J]. Journal of Nuclear Agricultural Sciences, 2019, 33(3): 490-497.
[15]	SHI Xinjie, WU Xiao, CHEN Yangyang, CAO Peng, BAI Bing, LI Mingzhi, YIN Hao, ZHANG Shaoling. The Biological Damage Effects of ⁶⁰Co-γ Ray Radiation on Cuiguan and Yuluxiang Pear Branches [J]. Journal of Nuclear Agricultural Sciences, 2019, 33(11): 2095-2102.