Journal of Nuclear Agricultural Sciences ›› 2017, Vol. 31 ›› Issue (9): 1693-1699.DOI: 10.11869/j.issn.100-8551.2017.09.1693

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles     Next Articles

Effect of 60Co-γ Irradiation on Capsule of Epidendium secundum

ZHOU Yaqian1, YAO Na1, WEI Li2, LI Lubin1, LIU Lei1, *   

  1. 1Research institute of Forestry, Chinese academy of forestry, Beijing 100091;
    2 Environmental Monitoring Center of Handan, Handan, Hebei 056002
  • Received:2016-08-17 Online:2017-09-10 Published:2017-07-18

60Co-γ射线对树兰蒴果辐照生物学效应研究

周亚倩1, 姚娜1, 魏莉2, 李潞滨1, 刘蕾1, *   

  1. 1中国林业科学研究院林业研究所,北京 100091;
    2 邯郸市环境监测中心站,河北 邯郸 056002
  • 通讯作者: 刘蕾,女,助理研究员,主要从事园林植物与观赏园艺研究。E-mail:liulei0828@126.com
  • 作者简介:周亚倩,女,主要从事园林植物与观赏园艺研究。E-mail:382373549@qq.com
  • 基金资助:
    国家科技支撑计划(2012BAD01B0702)

Abstract: To investigate the effect of the 60Co-γ ray treatment on the Epidendrum secundum capsule, different radiation doses of 60Co-γ ray (0 to 200 Gy) were used in this study, and the effect of radiation on the seeds germination and the seedling growth were investigated. The results showed that 20 Gy radiation dose can promote the germination rate compared with the control group, and the germination time was shortened. Under the radiation dose of 200Gy, the seeds could not germinate. The semi-lethal dose (LD50) was 78.08 Gy. The biological statistics analysis indicated the radiation dose had larger impact on plant height and leaf length, and had little effect on blade quantity and less influence on the leaf width. These results will lay the theory basis for radiation breeding of Epidendium secundum.

Key words: 60Co-γ, ray, Epidendrum secundum, capsule, mutation breeding, semi-lethal dose

摘要: 为探讨辐照处理对树兰种子的生物学效应,以树兰蒴果为研究对象,60Co-γ射线为辐照源,设置不同剂量(0~200 Gy)进行辐照处理, 在组织培养条件下研究其生物学效应。结果表明,当辐照剂量为20 Gy时可提高种子萌发率,并缩短种子萌发时间,而当辐照剂量为200 Gy时,种子不再萌发;树兰种子的半致死剂量为78.08 Gy。辐照处理对树兰再生植株的影响明显,与辐照剂量呈正相关,对株高、叶长影响最大,叶片数量次之,叶宽最小。本研究结果为树兰属植物离体诱变育种提供了一定的科学依据。

关键词: 60Co-γ, 射线, 树兰, 蒴果, 诱变育种, 半致死剂量