Journal of Nuclear Agricultural Sciences ›› 2016, Vol. 30 ›› Issue (8): 1453-1459.DOI: 10.11869/j.issn.100-8551.2016.08.1453

• Induced Mutations for Plant Breeding·Agricultural Biotechnology •     Next Articles

Cloning and Functional Analysis of SEP-like Gene From Phyllostachys violascens

LIU Shinan1,2, QI Tiantian2, MA Jingjing2, MA Lyuyi1, LIN Xinchun2   

  1. 1 Forestry College, Beijing Forestry University, Beijing 100083;
    2The Nurturing Station for State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin'an, Zhejiang 311300
  • Received:2016-01-19 Online:2016-08-27 Published:2016-07-08

雷竹SEP-like基因的克隆及功能分析

刘世男1,2, 戚田田2, 马晶晶2, 马履一1, 林新春2   

  1. 1北京林业大学林学院,北京 100083;
    2浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江 临安 311300
  • 通讯作者: 林新春,男,教授,主要从事植物生物技术研究。E-mail: lxc@zafu.edu.cn
  • 作者简介:刘世男,女,主要从事森林培育及竹子生物技术研究。E-mail: lsn_smile8866@126.com
  • 基金资助:
    国家自然基金(31270715,31000295),国家科技部973项目(2012CB723008)

Abstract: To study the functions of SEP-like genes in bamboo, a SEP homolog was isolated from Phyllostachys violanscens, and named as PvMADS5. The full-length ORF of PvMADS5 is 687bp and encod a protein of 228aa. Sequence alignment and phylogenetic analysis showed that PvMADS5 belongs to OsMADS5 subfamily and was homologous to SEP-like protein from Fargesia nitida, Phyllostachys edulis and Dendrocalamus latiflorus. Real-time quantitative PCR revealed PvMADS5 was expressed in all the tested organs of flowering and non-flowering bamboo plants. Based on subcelluar localization assay PvMADS5 was shown to be a nuclear protein. Overexpression PvMADS5 in wild-type Arabidopsis delayed flowering, and caused narrow and small rosetta leaves, suggesting that PvMADS5 might be a floral repressor and involved in the leaf development. This study will provide valuable information for further disclosing the mechanism of bamboo flowering.

Key words: Phyllostachys violascens, PvMADS5, expression pattern, flowering

摘要: 为探究竹类植物中SEP-like基因的功能,利用同源克隆方法从雷竹中克隆出1个SEP-like基因,命名为PvMADS5。该基因的开放阅读框为687bp,可编码228个氨基酸。同源比对与系统进化树分析表明,该蛋白与箭竹、毛竹、麻竹SEP类蛋白同源,同属于禾本科OsMADS5亚类。实时荧光定量PCR结果表明,PvMADS5在开花和不开花雷竹中的幼叶、老叶、秆、竹笋及花中均有表达;亚细胞定位表明PvMADS5是核蛋白。在拟南芥中过表达PvMADS5,转基因植株比野生型开花晚并且莲座叶变小,表明PvMADS5可能是开花抑制基因且参与叶的发育。本研究为揭示竹子开花的分子机制提供了理论依据。

关键词: 雷竹, PvMADS5, 组织特异性, 开花