Journal of Nuclear Agricultural Sciences ›› 2015, Vol. 29 ›› Issue (4): 777-785.DOI: 10.11869/j.issn.100-8551.2015.04.0777

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Comprehensive Evaluation of Resistance to Co(Ⅱ) of Four Kinds of Lawn Plant in Adult Stage

RUAN Chen1,2, CHEN Xiaoming1,2, LIU Xiaoling1, SONG Shou1, XU Yan1, WANG Chao1, HAO Xichao1, ZHANG Qian1, TONG Ke1   

  1. 1. Southwest university of science and technology, college of life science and engineering, Mianyang Sichuan 621010;
    2. Key Laboratory for National Defence China, Southwest university of science and technology, Mianyang Sichuan 621010
  • Received:2014-08-01 Revised:2014-12-11 Online:2015-04-13 Published:2015-04-16

4种草坪植物成苗期Co(Ⅱ)耐受性综合评价

阮晨1,2, 陈晓明1,2, 刘小玲1, 宋收1, 许燕1, 王超1, 郝希超1, 张倩1, 仝科1   

  1. 1. 西南科技大学生命科学与工程学院, 四川 绵阳 621010;
    2. 西南科技大学国防重点学科实验室, 四川 绵阳 621010
  • 通讯作者: 陈晓明, 女, 教授, 主要从事核废物辐射生物效应研究.E-mail:877753845@qq.com
  • 作者简介:阮晨, 男, 主要从事放射性元素污染土壤修复研究.E-mail:787935635@qq.com
  • 基金资助:

    国家自然科学基金资助项目(11075134),国防科技项目资助(14ZG6101),四川省生物质资源利用与改性工程技术研究中心基金资助(13zxsk02)

Abstract:

In order to evaluate the resistance to Co(II) of lawn plant, to establish a comprehensive evaluation method for resistance to Co(II) of lawn plant. Four kinds of lawn plant which have some growth advantages in Co(II) polluted environment were selected as experimental materials in this research. Comprehensive evaluation index include the height, aboveground biomass, membrane permeability, chlorophyll content, soluble protein, soluble sugar, free proline, MDA, and O2-contents after Co(II) stress. A comprehensive evaluation of resistance to Co(II) of these lawn plants in adult stage was established by using principal component analysis and its membership function. The results showed that the comprehensive evaluation values of resistance to Co(II) of four kinds of lawn plant, the pecking order is tall fescue, timothy grass, inflorescences, annual ryegrass. In this research, the resistance to Co(II) of tall fescue is better than the other plants. Though the resistance to Co(II) of the annual ryegrass is the worst on some others, the annual ryegrass plays an important part in heavy metals contaminated soil remediation, because of the advantage of its huge biomass. In this research, some pattern has been found by physiological and biochemical analysis. For four kinds of lawn plant, the values of membrane permeability, free proline, MDA, O2- contents increased as the increase of Co(II) concentrations. For inflorescences and annual ryegrass, the values of height, aboveground biomass, chlorophyll content, soluble protein and soluble sugar significant decreased in high Co(II) concentrations. For tall fescue, the values of chlorophyll content, soluble protein and soluble sugar increased at first and decreased later with the increase of Co(II) concentrations. For timothy grass, the values of chlorophyll content and soluble protein increased at first and decreased later. With the increase of Co(II) concentrations, the values of soluble sugar, by contrast, decreased at first and increased later. By using principal component analysis, transform nine physiological and biochemical indexes into two principal components. The contribution of two principal components is 96.36%. It is worthy of trying to find the plant which the ability of resistance to Co(II) is relatively good, to provide amount of repair methods and basis for remediating the soil contaminated by Co(II).

Key words: lawn grass, cobalt tolerance, principal component analysis, subordinate function values analysis, phytoremediation

摘要:

为了评估草坪植株的Co(Ⅱ)耐受性, 本研究通过对4种草坪植物苗期的株高、地上生物量、相对电导率、叶绿素含量、可溶性蛋白、可溶性糖、游离脯氨酸、丙二醛, O2-含量等生理生化指标的测定分析, 利用主成分分析法和隶属函数法对4种植物进行综合评判, 建立了植株Co(Ⅱ)耐受性综合评价体系.结果表明, 4种草坪植物的相对电导率、脯氨酸、丙二醛、超氧阴离子自由基等指标随着Co(Ⅱ)处理浓度的增加而升高, 各处理间存在着显著差异, 其他指标的规律各具特点.高羊茅的Co(Ⅱ)耐受性明显高于其他3种植物, 一年生黑麦草虽然Co(Ⅱ)耐受性最低, 但由于其巨大的生物量优势, 也具有较大的Co(Ⅱ)污染土壤修复潜力.本研究通过建立植物Co(Ⅱ)耐受性综合评价方法, 为寻找Co(Ⅱ)抗性优良植株, 对土壤Co(Ⅱ)污染的植物修复提供了一定的技术手段和科学依据.

关键词: 草坪植物, Co(Ⅱ)耐受性, 主成分分析法, 隶属函数法, 植物修复