Journal of Nuclear Agricultural Sciences ›› 2015, Vol. 29 ›› Issue (4): 704-709.DOI: 10.11869/j.issn.100-8551.2015.04.0704

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Enzymatic Saccharication and Fermentation of Irradiation-pretreated Rice Straw for Ethanol

CHEN Liang1, SU Xiaojun2, XIONG Xingyao2,3, CHEN Jingping1, WU Xiaofen1, HU Qiulong2, Zhang Yong1, WANG Keqin1   

  1. 1. Institute of Nuclear Agricultural Science & Space Mutation Breeding, Changsha, Hunan 410125;
    2. Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, Hunan 410128;
    3. The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Received:2014-06-20 Revised:2015-01-06 Online:2015-04-13 Published:2015-04-16

辐照预处理水稻秸秆酶解糖化与发酵产乙醇工艺

陈亮1, 苏小军2, 熊兴耀2,3, 陈静萍1, 武小芬1, 胡秋龙2, 张勇1, 王克勤1   

  1. 1. 湖南省核农学与航天育种研究所, 湖南 长沙 410125;
    2. 湖南农业大学湖南省作物种质创新与资源利用重点实验室, 湖南 长沙 410128;
    3. 中国农业科学院蔬菜花卉研究所, 北京 100081
  • 通讯作者: 王克勤, 男, 研究员, 主要从事核技术在生物质能源中的应用研究.Email:wkq6412@163.com
  • 作者简介:陈亮, 男, 助理研究员, 主要从事核技术在生物质能源中的应用研究.Email:chenliang912@163.com
  • 基金资助:

    国家“863”计划课题(2012AA101804),湖南省科学技术厅科研条件创新专项(湘科条字{2013}68号),湖南省农业科学院科技创新专项

Abstract:

To investigate the feasibility of ethanol production from irradiation-pretreated lignocelluloses without detoxification, irradiation-pretreated rice straw was subjected to enzymatic hydrolysis or simultaneous saccharification and fermentation with comparison of water-washed substrate. The results revealed that there were no obvious difference of the cellulose conversion and ethanol conversion between detoxification treatment and control at a substrate concentration of 15%. After 72 h incubation, the cellulose conversion and ethanol conversion of irradiation-pretreated rice straw were 64.11% and 65.70%, respectively, with corresponding 63.50% and 67.45% of water-washed substrate. According to materials banlance, it can obtained 11.04 g ethanol from 100 g rice straw with irradiation pretreatment and 9.62 g ethanol with irradiation and water-washing pretreatment, which provides a guidance for the application of irradiation in fuel ethanol.

Key words: irradiation, rice straw, ethanol

摘要:

为探讨辐照预处理后木质纤维素在未脱毒的前提下发酵产乙醇的可行性, 本研究以水洗脱毒的水稻秸秆为对照, 对未采取脱毒处理的水稻秸秆进行酶解糖化与发酵产乙醇研究.结果表明:在15%底物浓度下, 水洗脱毒对辐照水稻秸秆在酶解转化率和乙醇转化率没有明显提高, 酶解72h后辐照秸秆和对照的纤维素转化率分别为64.11%和63.50%, 同步糖化发酵72h后乙醇转化率分别为65.70%和67.45%;经物料衡算, 100g水稻秸秆原料经射线辐照预处理或辐照水洗脱毒预处理后, 再进行糖化发酵, 可分别获得11.04g和9.62g乙醇.本研究为辐照在燃料乙醇领域的应用提供了参考.

关键词: 辐照, 水稻秸秆, 乙醇