Journal of Nuclear Agricultural Sciences ›› 2015, Vol. 29 ›› Issue (4): 643-650.DOI: 10.11869/j.issn.100-8551.2015.04.0643

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Genome-wide Analysis of DGAT Gene Family in Maize

LI Shuxia1, LIU Wei1, LI Wei1,2, ZHU Shuijin1, WU Dianxing1, CHEN Jinhong1   

  1. 1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310058;
    2. Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan 455000
  • Received:2014-04-14 Revised:2014-10-21 Online:2015-04-13 Published:2015-04-16

玉米DGAT基因家族的全基因组分析

李书霞1, 刘伟1, 李威1,2, 祝水金1, 吴殿星1, 陈进红1   

  1. 1. 浙江大学农业与生物技术学院, 浙江 杭州 310058;
    2. 中国农业科学院棉花研究所, 河南 安阳 455000
  • 通讯作者: 陈进红, 男, 副教授, 主要从事作物品质调控与种质创新研究.E-mail:jinhongchen@zju.edu.cn
  • 作者简介:李书霞, 女, 主要从事作物种质创新研究.E-mail:lishuxia2008qau@163.com
  • 基金资助:

    转基因生物新品种培育科技专项(2013ZX08005-005),中央高校基本科研业务费专项资金(2014FZA6011)

Abstract:

Three acyl glycerin is the main storage in plant oil, and DGAT (Diacylglycerol acyltransferase) gene plays an important role in three acyl glycerol synthesis. With the widely application of the plant oil, maize oil research was paid more and more attentions. In order to further understand DGAT gene family, the identification and classification were done using bioinformatics methods, and moreover the system evolution, gene structure and expression patterns were also analyzed. The results showed that 7 DGAT genes in maize could be divided into 3 subgroups, ZmDGAT1, ZmDGAT2 and ZmDGAT3, distributed on chromosome 4, 5, 6, 9 and 10. The gene structure and transmembrane domains of different subgroups had significant difference and the gene expressions had also certain differences. ZmDGAT1 gene was mainly expressed in the endosperm, ZmDGAT2 gene expression had a wider range, and the expression of ZmDGAT3 gene was mainly in the blade. In this study, the gene structure and expression of DGAT gene family were definitely known, and laid the foundation for understanding DGAR gene function and further improving the plant oil content.

Key words: maize, DGAT gene family, gene structure, gene expression

摘要:

为了进一步了解玉米中DGAT基因家族, 本文利用生物信息学方法对玉米DGAT基因家族进行了鉴定和分类, 并对其系统进化、基因结构以及表达模式进行了分析.结果表明:玉米中7个DGAT基因可以分成ZmDGAT1、ZmDGAT2和ZmDGAT3 3个亚组, 分布在第4、5、6、9和10这5条染色体上, 不同亚组的基因结构和跨膜区有明显差别, 其表达也有一定差异, 其中ZmDGAT1主要在胚乳中表达, ZmDGAT2表达范围较广, 而 ZmDGAT3的表达主要集中在叶片.研究明确了玉米中DGAT基因家族的基因结构和表达情况, 为了解DGAT基因功能以及进一步提高植物油分含量提供了理论基础.

关键词: 玉米, DGAT基因家族, 基因结构, 基因表达