Journal of Nuclear Agricultural Sciences ›› 2015, Vol. 29 ›› Issue (4): 637-642.DOI: 10.11869/j.issn.100-8551.2015.04.0637

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Research on the Factors Influencing Regeneration of Plantlets From Resistant Calli of Transgenic Maize

ZHANG Zhiyong1,2, LI Wanchen2, FU Fengling2   

  1. 1. Key laboratory of Regional Charactoristic Agricultral Resources, Depatment of Education/College of Life Science, Neijiang Normal University, Neijiang, Sichuan 641100;
    2. Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130
  • Received:2014-03-04 Revised:2014-12-21 Online:2015-04-13 Published:2015-04-16

转基因玉米抗性愈伤再生植株的影响因素研究

张志勇1,2, 李晚忱2, 付凤玲2   

  1. 1. 内江师范学院生命科学学院/四川省高等学校特色农业资源研究与利用重点实验室, 四川 内江 641100;
    2. 四川农业大学玉米研究所, 四川 成都 611130
  • 通讯作者: 付凤玲, 女, 教授, 研究方向为玉米遗传育种与生物技术研究.E-mail:ffl@sicau.edu.cn
  • 作者简介:张志勇, 男, 副研究员, 研究方向为植物分子生物学.E-mail:zhangzyong219@126.com
  • 基金资助:

    国家转基因生物新品种培育科技重大专项项目(2009ZX08003012B,2013ZX08003-004),四川省教育厅高校科研创新团队项目(14TD0025),四川省科技厅基础研究项目(2015JY0242),四川省教育厅重点项目(13ZA0007,14ZA0250),内江师范学院自然科学重点项目(13ZA01),内江师范学院博士科研基金(13B03)

Abstract:

Culture of regenerated plants is one of the most important procedures in transgenic maize research. In this paper, basic culture medium, the size of embryonic calli, subculture times and hormone supplements affecting differentiation and regeneration of transgenic maize were discussed. The results showed that basic N6 medium had an improvement in the differentiation of resistant calli. The highest differentiation rate was achieved when the resistant calli size was about 2.5 to 3.5 mm and with subculture times 2 to 3 times. Addition of ABT root-promoting powder (0.5 mg·L-1) and uniconazole S3307 (0.25 mg·L-1) in regeneration medium could promote rooting and seedling strengthening of regenerated plants. This research laid a basis for establishing transgenic maize regeneration system.

Key words: maize, transgene, Agrobacterium tumfacions, culture medium, regenerated plants

摘要:

再生植株的培养是玉米转基因重要环节之一.本文比较了基本培养基、愈伤组织大小、继代次数、激素添加物对转基因抗性愈伤组织分化和再生植株的影响.结果表明:以N6为基本培养基有利于抗性愈伤组织分化;抗性愈伤组织大小在2.5~3.5mm之间、继代2~3次的分化率最高;再生培养基中添加ABT 0.5 mg·L-1和S3307 0.25 mg·L-1能促进生根壮苗.本研究为转基因玉米再生体系建立提供了依据.

关键词: 玉米, 转基因, 农杆菌, 培养基, 再生植株