Journal of Nuclear Agricultural Sciences ›› 2015, Vol. 29 ›› Issue (4): 629-636.DOI: 10.11869/j.issn.100-8551.2015.04.0629

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Screening and Identification of a Small Round Seed and Semi-dwarf Mutant srd-1 of Native Rice Variety Lailong in Guizhou

LI Lei, ZENG Xiaofang, ZHAO Degang   

  1. Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education/Guizhou Collaborative Innovation Center for Mountain Ecology & Agro-Bioongineering, Guizhou University, Guiyang, Guizhou 550025
  • Received:2014-04-04 Revised:2014-10-21 Online:2015-04-13 Published:2015-04-16

贵州地方稻种来拢小圆粒半矮秆突变体srd-1筛选及鉴定

李磊, 曾晓芳, 赵德刚   

  1. 贵州大学山地植物资源保护与种质创新省部共建教育部重点实验室/贵州省山地生态与农业生物工程协同创新中心, 贵州 贵阳 550025
  • 通讯作者: 赵德刚, 男, 教授, 主要从事植物基因工程与转基因生物安全研究, Email:dgzhao@gzu.edu.cn
  • 作者简介:李磊, 男, 主要从事水稻基因工程方面研究.Email:lilei3390@126.com;曾晓芳, 女, 主要从事植物生物调控研究.Email:zengxiaofang@foxmail.com
  • 基金资助:

    国家转基因生物新品种培育科技重大专项“安全转基因技术研究”(2013 ZX 08010-003,2014 ZX 08010-003),贵州省科技重大专项“贵州特有水稻种质资源优异基因克隆及育种技术研究应用”(黔科合重大专项字[2012]6005号)

Abstract:

In this paper, the mutant library was built from a Guizhou native rice variety‘Lailong’using EMS treatment, from which the small round seed and semi-dwarfism characteristics of mutant srd-1 was identified, and the molecular mechanism of small round seed and semi-dwarf traits were studied.The skotomorphogenesis showed that the dwarf trait of srd-1 mutant was not related to the BR. srd-1 mutant performance was sensitive to GA. Exogenous GA\-3 could promote the growth of seedling's second leaf sheath of srd-1, induce the activity of α- amylase and rescue the tilt arrangement of cell microtubules skeleton back to wild type transverse arrangement, which indicated that the dwarf character was not related to GA signal transduction pathway. The expressions of key enzyme genes OsKS1 and OsGA2ox1 in GA synthesis pathway had no significant differences compared with wild type, but the gene expressions OsCPSOsKO2、OsKAOOsGA20ox2 and OsGA3ox2 were down-regulated, which preliminarily indicated that the dwarf trait of srd-1 mutant might be due to the activity decrease of GA biosynthesis. In addition, the expressions of small round seed control genes SRS1、SRS3 and SRS5 exhibited down-regulated. This suggested that the mutant gene of srd-1 mutant simultaneously involved in the regulation of grain size and plant height, affected activity GA biosynthesis and the expressions of small round seed key enzyme regulated genes, and then led tothe dwarf and small round seed phenotype. The study provided a reference for the mutant gene mapping and using research.

Key words: ‘Lailong’, Srd-1 mutant, screening and identification

摘要:

以贵州地方稻种来拢突变体库中能稳定遗传的小圆粒半矮秆突变体srd-1为材料, 对其半矮化和小圆粒性状分子机制进行研究.暗形态建成表明srd-1突变体矮化性状与BR无关.srd-1突变体表现对GA敏感, 外源GA3对突变体幼苗第2叶鞘有促进生长作用, 能诱导α-淀粉酶活性, 可以使倾斜方向排列的细胞微管骨架恢复至野生型横向排列方向, 表明srd-1突变体矮化性状与GA信号传导途径无关.GA合成途径中关键酶基因OsKS1和OsGA2ox1表达相对于野生型差异不显著, OsCPSOsKO2、OsKAOOsGA20ox2和OsGA30ox2基因表达下调, 初步说明srd-1突变体的矮化性状可能是由活性GA生物合成降低所致.此外, 小圆粒调控基因SRS1、SRS3和 SRS5表达下调, 表明srd-1突变体突变基因同时参与了籽粒大小和株高的调控, 突变基因影响了活性GA的生物合成和小圆粒调控关键酶基因的表达, 进而导致了矮化和小圆粒表型的产生.该研究为突变体突变基因的定位及利用研究提供了参考.

关键词: 来拢, srd-1突变体, 筛选与鉴定