J4 ›› 2010, Vol. 24 ›› Issue (1): 187-191.DOI: 10.11869/hnxb.2010.01.0187

• 论文 • Previous Articles     Next Articles

UTILIZATION OF TRITIATED WATER DILUTION TECHNIQUE IN DETERMINATION OF NITROGEN PARTITIONING IN CASHMERE GOATS

  

  1. 1.College of Animal Science and Veterinary Medicine,Henan Agricultural University, Zhengzhou, Henan, 450002; 2. Life Science Research Center of Henan Agricultural University, Zhengzhou, Henan, 450002; 3. Yong Cheng Animal Husbandry Bureau, Yong Cheng , Henan, 476600;4.Henan Hongbang Biotechnology Co., Ltd, Henan, Xin Zheng, Henan, 451162
  • Received:2009-05-30 Online:2010-02-20 Published:2010-03-22

氚水稀释技术在测定绒山羊氮分配中的应用研究

王林枫  杨改青  刘 平  张世君   

  1. 1. 河南农业大学牧医工程学院,河南 郑州 450002;2. 河南农业大学生命研究中心,河南 郑州 450002;3. 河南省永城市畜牧局,河南 永城 476600;4. 河南宏邦生物科技有限公司,河南  新郑 451162
  • 通讯作者: 王林枫(1968-),男,河南汝阳人,博士,副教授,从事动物营养生理与泌乳研究。Tel:13938484563
  • 作者简介:王林枫(1968-),男,河南汝阳人,博士,副教授,从事动物营养生理与泌乳研究。Tel:13938484563;E-mail:wanglf1968@126.com
  • 基金资助:

    河南农业大学博士科研启动基金(30300098)

Abstract:

In order to investigate nitrogen partitioning in local cashmere goats ,six Inner Mogolia White Cashmere goats between 2 to 2.5 years old were used to determine the nitrogen partitioning in cashmere goats. The total retained nitrogen(TN)in body, distribution of body nitrgen and hair nitrogen were measured by general digestive and metabolism method combined with tritiated water dilution technique. Results showed that the above methods were ideal for determining body nitrgen(BN)and hair nitrogen (fur nitrogen, FN)of Cashmere goats. There are obvious significances between BN and FN in different seasons. In telogen, BN and FN partitioning was 75.7±0.62% and 24.3±0.62%, respectively. Whereas, it was changed to 66.6±2.2% and 33.4±2.2% in anagen. BN partitioning was decreased when the season changed from telogen to anagen, while the FN partitioning was increased, which indicated that more nitrogen substance was partitioned to body growth in telogen, and more nitrogen substance was distribute to cashmere growth in anagoen. These transformation were related to the changing of photoperiod and some hormones, such as, melatonin(MT), prolactin(PRL) and IGF-I. It can be concluded that tritiated water dilution technique could be used to detect body protein content as well as BN, combining general digestive and metabolism experiment, FN partitoning can be determined. BN and FN partitoning varied with the season in cashmere goats because of hormones changing.

Key words: cashmere goat, isotope, tritiated water, dilution technique, nitrogen partitioning  

摘要:

为了研究含氮物质在绒山羊体内的分配规律,本文选择年龄在2~2.5周岁的绒山羊羯羊,采用消化代谢和氚水稀释技术结合的方法测定不同时期绒山羊总氮沉积量、体氮和毛绒氮的分配量。结果表明,用消化代谢和氚水稀释技术可以准确地测定绒山羊的体氮和毛绒氮的分配量,不同时期的体氮和毛绒氮的分配比例有较大差异。在非生绒期和生绒旺盛期,体氮的分配比例分别为75.7%±0.62%和66.6%±2.2%,毛绒氮的分配比例分别为24.3%±0.62%和33.4%±2.2%,表明不同时期绒山羊的生长方向发生变化。同时发现内分泌也随季节发生变化,主要表现为非生绒期PRL(催乳素)和IGF-I(类胰岛素生长因子Ⅰ)的水平高,MT(褪黑素)的水平低;生绒旺盛期PRL和IGF-I的水平低,MT的水平高。证明了氚水稀释技术在测定动物氮分配中的可行性,揭示了不同时期含氮物质在绒山羊体内分配的变化规律以及内分泌在这些变化中的调节作用,为在不同季节对绒山羊采取合适的饲养管理提供了理论依据。

关键词: 绒山羊, 同位素, 氚水, 稀释技术, 氮分配