J4 ›› 2010, Vol. 24 ›› Issue (1): 181-186.DOI: 10.11869/hnxb.2010.01.0181

• 论文 • Previous Articles     Next Articles

COMPARISON OF DETERMING METHODS FOR METHIONINE EFFECT ON ENDOGENOUS FLOWS OF NITROGEN AND AMINO ACIDS IN GROWING GOATS

  

  • Received:2009-05-27 Online:2010-02-20 Published:2010-03-22

蛋氨酸对山羊内源氮和氨基酸流量影响的测定方法比较

  

  1. 中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,湖南 长沙 410125
  • 通讯作者: 谭支良(1967-),男,湖南湘乡人,研究员,博士生导师,主要从事农牧复合生态系统研究。
  • 作者简介:周传社(1980-),男,湖南汨罗人,博士,助理研究员,主要从事反刍动物营养与环境安全研究。Tel:0731-84615236;E-mail: shezhang122@yahoo.com.cn
  • 基金资助:

    国家“十一五科技支撑”课题(2008BADA7B04)

Abstract:

This experiment was conducted to study the effects of dietary methionine (Met) levels on the duodenal flows of endogenous N (EN) and endogenous amino acids (ENAA) in growing goats, and to compare the results gained from the difference method and isotope dilution method by feeding normal protein-containing diets. Three tested goats (20 kg ± 2.5 kg) were fitted with a ruminal, duodenal cannulae and the jugular catheters, and allocated to three dietary treatments in a 3 × 3 Latin square design. The dietary treatments consisted of a total mixed ration containing three levels of Met (0.15%, 0.25% and 0.35%), respectively. The results showed that the duodenal flows of EN and ENAA were the lowest when the dietary Met level was 0.15%, and there was no significant (p>0.05) difference in the duodenal flows of EN and ENAA measured by the difference method and isotope dilution method. The duodenal flows of EN and ENAA determined by the difference method was lower (p>0.05) than the data by the isotope dilution method. It is concluded that the duodenal flows of EN and ENAA are not affected by the dietary direct supplementation of Met, and that the duodenal flows of EN and ENAA determined by the difference method were lower than those by the isotope dilution method.

Key words: methionine, difference method, isotope dilution method, endogenous nitrogen, endogenous amino acids, flows, goats

摘要:

采用3×3拉丁方试验设计,选用3头安装有永久性瘤胃、十二指肠近端瘘管和颈静脉插管,平均体重为20 kg±2.5 kg的健康湘东黑山羊为试验动物,饲喂3种Met水平分别为0.15%、0.25%和0.35%的试验日粮,研究了Met水平对山羊内源氮(EN)和内源氨基酸(ENAA)流量的影响,同时比较差量法和同位素稀释法测定山羊十二指肠EN和ENAA流量的差异。结果显示:日粮Met水平对山羊十二指肠EN和ENAA流量没有显著影响(P>0.05);当日粮Met水平为0.15%时,差量法和同位素稀释法测定十二指肠EN和ENAA流量均最低,且2种测定方法之间无显著差异(P>0.05);差量法测定EN和ENAA流量均低于同位素稀释法测定结果(P>0.05)。结果表明:山羊十二指肠EN和ENAA流量不受日粮Met水平影响;差量法测定的十二指肠EN和ENAA流量低于同位素稀释法。

关键词: 蛋氨酸, 差量法, 同位素稀释法, 内源氮, 内源氨基酸, 流通量, 山羊